Smart Fluo-Sensor

high-resolution terminology - matching measurements at high-resolution

Smart Fluo-Sensor

Description

Smart Fluo-Sensor: LED with specified excitation wavelength (Smart Fluo-Sensor Green, 525 nm, and Smart Fluo-Sensor Blue, 465 nm), photodiode, Filter-Cap attached with specific optical filters for the LED and photodiode. Smart Fluo-Sensors are precalibrated with sensor-specific memory and direct input into DatLab 7.

Reference: O2k-FluoRespirometer

MitoPedia methods: Fluorometry 

MitoPedia O2k and high-resolution respirometry: O2k hardware 

Orientation for connection

OK, fully inserted

WRONG, not fully inserted

Horizontal cable routing

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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O2k-Guide

Select the Smart Fluo-Sensors

Switching between different excitation wavelengths and filters is achieved by simply exchanging the Smart Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters.

Select the Fluo-Sensor and filter set form the table on application-specific settings. Each Fluo-Sensor is delivered with a mounted filter set:

1. Smart Fluo-Sensor Green: 525 nm (dominant wavelength). The installed Filter-Cap is ready for H2O2 measurement with Amplex® UltraRed.
2. Smart Fluo-Sensor Blue: 465 nm (dominant wavelength). The installed Filter-Cap is ready for measurements of mt-membrane potential with safranin. A different filter is used for measurement with Magnesium green® or Calcium green®.

O2k-Guide: Setup - next step Mounting a Filter-Cap

Connect Smart Fluo-Sensor to O2k

Connect the Smart Fluo-Sensor cable to the Fluo plug of the O2k, insert the male plug of the cable into the female Fluo plug. The red dot on the male plug faces straight upwards. Each Smart Fluo-Sensor can be used on O2k-Chamber A or B. The blue frame of the chamber window and the Smart Fluo-Sensor are specially designed to align in a specific rotational position with the cable extending horizontally to left (chamber A) and right (chamber B). The Smart Fluo-Sensor is carefully inserted into the window frame and rotated into final position, leaving no gap between window frame and sensor body.

To remove the Smart Fluo-Sensor, carefully pull out the sensor body with slight back and forth rotations. Do not pull on the cable.

Stoppers

Use only black PEEK stoppers in conjunction with fluorometric measurements. The black stoppers can be used for all HRR applications.

MitoPedia methods: Fluorometry