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Property "Has-20abstract" (as page type) with input value "-5B-5BImage:MITOEAGLE-2Dlogo.jpg-7Cleft-7C100px-7Clink=http:-2F-2Fwww.mitoeagle.org-2Findex.php-2FMitoEAGLE-7CCOST-20Action-20MitoEAGLE-5D-5D-0ATargeting-20characteristic-20metabolic-20modes-20to-20drain-20bioenergetics-20in-20cancer-20cells-20has-20recently-20gained-20attention.-20To-20increase-20the-20potential-20of-20this-20strategy,-20drugs-20must-20be-20drastic-20inhibitors-20of-20all-20metabolic-20pathways-20in-20cancer-20cell-20subpopulations,-20and-20importantly,-20be-20targeting-20malignant-20cells-20selectively.-20We-20exploited-20enhanced-20expression-20of-20asialoglycoprotein-20receptors-20on-20human-20hepatocellular-20carcinoma-20HepG2-20cell-20membrane-20for-20specific-20delivery-20of-20an-20effective-20metabolic-20inhibitor-20loaded-20in-20nanoparticles-20conjugated-20with-20galactosylated-20chains-20as-20recognition-20termini.-20Submicromoles-20of-20iodoacetate-2Dloaded-20nanoparticles-20(NIA)-20were-20sufficient-20to-20completely-20disrupt-20glycolytic-20as-20well-20as-20mitochondrial-20metabolism,-20causing-20substantial-20cytotoxicity-20of-20HepG2-20cells-20within-204-20h.-20To-20identify-20the-20mechanism-20of-20cell-20death-20by-20NIA,-20we-20performed-20extensive-20metabolic-20investigations-20of-20mitochondria-20stress-20in-20intact-2Dattached-20or-20permeabilized-2Dsuspended-20HepG2-20cells-20as-20well-20as-20on-20isolated-20mitochondria.-20Metabolic,-20flow-20cytometric,-20and-20molecular-20studies-20provided-20converging-20evidence-20that-20NIA-20triggers-20complete-20cell-20death-20through-20mitochondrial-20ROS-2Dmediated-20apoptosis-20induction-20concomitant-20with-20bioenergetic-20deprivation-20in-20HepG2-20and-20HuH-2D7-20but-20not-20in-20normal-20WI-2D38-20cells.-20Imaging-20studies-20confirmed-20lower-20uptake-20of-20NIA-20by-20normal-20cells-20and-20their-20mitochondria-20relative-20to-20cancer-20cells-20which-20highlight-20the-20targetability-20of-20cancer-20cell-20mitochondria-20by-20the-20current-20combination.-20Overall,-20our-20results-20revealing-20the-20ability-20of-20relatively-20low-20NIA-20concentrations-20to-20completely-20disrupt-20various-20metabolic-20pathways-20that-20are-20crucial-20for-20proliferating-20as-20well-20as-20resilient-20cancer-20cells-20provide-20a-20new-20treatment-20approach-20via-20nanoparticle-2Dassisted-20metabolic-20interventions." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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