Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Search by property

From Bioblast

This page provides a simple browsing interface for finding entities described by a property and a named value. Other available search interfaces include the page property search, and the ask query builder.

Search by property

Property "Has-20abstract" (as page type) with input value "-5B-5BImage:MITOEAGLE-2Dlogo.jpg-7Cleft-7C100px-7Clink=http:-2F-2Fwww.mitoglobal.org-2Findex.php-2FMITOEAGLE-7CCOST-20Action-20MitoEAGLE-5D-5D-0AMitochondria-20have-20significant-20impact-20on-20the-20suppleness-20for-20cancer-20cell-20proliferation-20and-20survival-20in-20stress-20environments,-20for-20example,-20nutrient-20diminution,-20hypoxia,-20and-20during-20cancer-20treatments.-20-20Mitochondria-20are-20the-20most-20important-20supply-20of-20reactive-20oxygen-20species-20(ROS),-20which-20are-20taken-20part-20in-20a-20vital-20role-20in-20mitochondria-20mediated-20apoptosis-20and-20mitochondrial-20aerobic-20respiration-20-5B1-5D.-20Moreover,-20in-20energy-20stress-20condition,-20AMPK-20activation-20aggravates-20cell-20survival-20via-20redox-20regulation-20for-20overcoming-20the-20metabolic-20stress-20-5B2-5D.-20Thus,-20the-20aim-20of-20the-20present-20study-20was-20to-20investigate-20the-20chemopreventive-20effects-20of-20Manuka-20honey-20(MH)-20by-20targeting-20mitochondrial-20dysfunction,-20while-20mitochondria-20play-20as-20an-20important-20mediator-20of-20tumorigenesis.-0A-0AMH-20is-20a-20good-20source-20of-20natural-20bioactive-20compounds-20and-20antioxidant-20capacity-20-5B3-5D.-20Human-20colon-20cancer-20Lovo-20cells-20were-20cultured-20in-20the-20presence-20of-20MH-20at-20different-20concentrations-20(0-20to-2050-20mg-2F-20mL)-20for-20up-20to-2072-20h-20and-20the-20cell-20viability-20percentage-20was-20evaluated-20by-20MTT-20assay.-20Taliยฎ-20image-20based-20Cytometer-20was-20used-20for-20evaluating-20the-20ROS-20production-20and-20apoptosis-20rate.-20Seahorse-20Bioscience-20XF24-20Extracellular-20Flux-20Analyzer-20was-20used-20for-20detection-20of-20oxygen-20consumption-20rate-20(OCR)-20as-20an-20indicator-20of-20mitochondrial-20oxidative-20phosphorylation-20by-20sequential-20adding-20of-20oligomycin,-202,4-2DDNP-20and-20rotenone-2F-20antimycin-20and-20extracellular-20acidification-20rate-20(ECAR)-20as-20an-20indicator-20of-20glycolysis-20by-20adding-20rotenone,-20glucose-20and-202-2DDG.-20The-20sensitive-20measurement-20of-20glycolysis-20and-20several-20parameters-20of-20mitochondrial-20function-20from-20adherent-20intact-20cultured-20cells-20were-20measured.-20Western-20blotting-20analysis-20was-20performed-20for-20detection-20of-20the-20protein-20expression-20of-20p53,-20caspase-2D3-20and-20AMPK-20pathway.-20-0A-0AMH-20suppressed-20the-20LoVo-20cells-20proliferation-20at-20dose-20and-20time-20dependent-20manner.-20It-20increased-20the-20intracellular-20ROS-20production-20and-20apoptotic-20rate-20of-20the-20LoVo-20cells,-20while-20the-20expression-20of-20apoptosis-20inducer,-20p53-20and-20caspase-2D3-20also-20increased-20after-20MH-20treatment.-20Seahorse-20analysis-20showed-20that-20MH-20markedly-20decreased-20mitochondrial-20function-20by-20reducing-20oxidative-20phosphorylation-20as-20an-20indicator-20of-20OCR-20(Figure-201A).-20The-20basal-20respiration,-20maximal-20oxygen-20consumption,-20spare-20respiratory-20capacity,-20ATP-2Dlinked-20respiration-20and-20proton-20leak,-20associated-20with-20cell-20survival,-20were-20also-20decreased-20after-20MH-20treatment-20compared-20to-20control-20(Figure-201B).-20In-20addition,-20it-20reduced-20the-20glycolysis-20function-20and-20suppressed-20the-20AMPK-20pathway-20expression-20as-20a-20consequence-3B-20it-20inhibited-20the-20production-20of-20ATP-20under-20metabolic-20stress.-20-0A-0AOur-20result-20suggested-20that-20mitochondrial-20dysfunction-20is-20one-20of-20the-20prime-20therapeutic-20tenures,-20while-20MH-20is-20a-20possible-20elevator-20of-20ROS-20production-20in-20order-20to-20activated-20apoptosis-20and-20disturbed-20bioenergetics-20phenotype-20of-20colon-20cancer-20cells-20may-20contribute-20to-20preclinical-20and-20clinical-20appraisal-20for-20cancer-20therapy." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

Showing below up to 0 results starting with #1.

View (previous 50 | next 50) (20 | 50 | 100 | 250 | 500)


    

List of results

No results.