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Property "Has-20abstract" (as page type) with input value "-5B-5BImage-2D3AMITOEAGLE-2Dlogo.jpg-7Cleft-7C100px-7Clink=http-2D3A-2F-2Fwww.mitoeagle.org-2Findex.php-2FMitoEAGLE-7CCOST-20Action-20MitoEAGLE-5D-5D-0AAs-20WG2-20leader-20in-20the-20MitoEAGLE-20COST-20Action-20my-20main-20role-20is-20to-20stimulate,-20engage-20and-20coordinate-20scientists-20in-20the-20different-20tasks-20to-20achieve-20our-20proposed-20deliverables.-20In-20this-20specific-20WG-20we-20should-20deliver-2D3A-0A-2D3A-2D3A-2D3A-2D3A-23A-20mitochondrial-20database-20on-20muscle-20tissue-20from-20humans-20and-20model-20organisms.-0A-2D3A-2D3A-2D3A-2D3A-23A-20set-20of-20guidelines-20for-20future-20studies-20on-20mitochondrial-20bioenergetics-20in-20muscle-20tissue.-0A-2D3A-2D3A-2D3A-2D3A-23Draft-20of-20review-20manuscripts-20on-20these-20topics.-0A-0AIn-20relation-20to-20MitoEAGLE-20data-20repository-20in-20muscle-20tissues,-20during-20my-20presentation-20I-20will-20summarize-20our-20progress-20in-20the-20generation-20of-20reference-20values-20for-20mitochondrial-20respirometry-20in-20permeabilized-20skeletal-20muscle-20sample-20preparations.-20The-20goal-20is-20that-20new-20researchers-20in-20the-20field-20follow-20a-20reference-20protocol-20and-20check-20if-20their-20values-20are-20in-20an-20acceptable-20range.-20This-20approach-20could-20serve-20to-20test-20researchers’-20technical-20skills-20and-20therefore-20determine-20if-20they-20are-20proficient-20enough-20to-20perform-20their-20own-20experiments-20with-20confidence.-0A-0AWe-20initially-20ran-20a-20pilot-20study-20including-207-20European-20groups.-20The-20results-20obtained-20show,-20as-20we-20expected,-20low-20variability-20among-20laboratories.-20After-20some-20adjustments-20and-20identifying-20some-20relevant-20steps-20that-20need-20to-20be-20carefully-20taken-20into-20consideration,-20the-20experimental-20procedure-20and-20results-20were-20judged-20satisfactory.-20Therefore,-20during-20Spring-202018-20a-20new-20invitation-20was-20broadly-20open-20to-20laboratories-20worldwide.-20The-20final-20aim-20is-20to-20collect-20all-20the-20data-20and-20write-20a-20manuscript-20by-202020.-20-0A-0AAll-20research-20groups-20involved-20obtained-20respirometry-20reference-20values-20in-20permeabilized-20soleus-20fibers-20from-20males-20(N=4)-20and-20females-20(N=4)-20C57BL-2F6J-20mice-20aged-2014-2D16-20weeks.-20The-20substrate-2Duncoupler-2Dinhibitor-20titration-20(SUIT)-20protocol-20used-20in-20the-20study-20is-20represented-20in-20Figure-201-20-5B1-5D.-20The-20presentation-20will-20give-20detailed-20information-20on-20the-20experimental-20procedures-20followed-20to-20fulfill-20our-20objective.-20Finally,-20we-20will-20present-20and-20discuss-20our-20preliminary-20data,-20including-20results-20from-20the-20second-20phase-20where-20ten-20additional-20international-20laboratories-20have-20performed-20the-20experiments.-0A-0AThis-20unique-20international-20study-20has-20the-20ambition-20to-20significantly-20contribute-20to-20major-20aims-20and-20concerns-20in-20the-20scientific-20community,-20as-20stablished-20more-20precisely-20in-20the-20MitoEAGLE-20memorandum-20of-20understanding-2D3A-20-20-0A-0A-2D3AData-20sharing-0A-2D3A-23Intensify-20the-20dissemination-20of-20updated-20knowledge-20and-20know-2Dhow-20among-20the-20partners.-0A-2D3A-23Mapping-20mitochondrial-20physiology-20and-20pathology-20to-20develop-20a-20mitochondrial-20database.-20-20-0A-2D3AReproducibility-20crisis-0A-2D3A-23MitoEAGLE-20recommendations-2D3A-20our-20goal-20is-20to-20increase-20the-20value-20and-20reduce-20the-20noise-20in-20mitochondrial-20research.-0A-2D3A-23Optimally-20harmonize-20protocols-20across-20research-20groups." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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