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Property "Has-20abstract" (as page type) with input value "Adaptation-20to-20different-20nutritional-20environments-20is-20essential-20for-20life-20cycle-20completion-20by-20all-20-27-27Trypanosoma-20brucei-27-27-20sub-2Dspecies.-20In-20the-20tsetse-20fly-20vector,-20L-2Dproline-20is-20among-20the-20most-20abundant-20amino-20acids-20and-20is-20mainly-20used-20by-20the-20fly-20for-20lactation-20and-20to-20fuel-20flight-20muscle.-20The-20procyclic-20(insect)-20stage-20of-20-27-27T.-20b.-20brucei-27-27-20uses-20L-2Dproline-20as-20its-20main-20carbon-20source,-20relying-20on-20an-20efficient-20catabolic-20pathway-20to-20convert-20it-20to-20glutamate,-20and-20then-20to-20succinate,-20acetate-20and-20alanine-20as-20the-20main-20secreted-20end-20products.-20Here-20we-20investigated-20the-20essentiality-20of-20an-20undisrupted-20proline-20catabolic-20pathway-20in-20-27-27T.-20b.-20brucei-27-27-20by-20studying-20mitochondrial-20Δ1-2Dpyrroline-2D5-2Dcarboxylate-20dehydrogenase-20(TbP5CDH),-20which-20catalyzes-20the-20irreversible-20conversion-20of-20gamma-2Dglutamate-20semialdehyde-20(γGS)-20into-20L-2Dglutamate-20and-20NADH.-20In-20addition,-20we-20provided-20evidence-20for-20the-20absence-20of-20a-20functional-20proline-20biosynthetic-20pathway.-20TbP5CDH-20expression-20is-20developmentally-20regulated-20in-20the-20insect-20stages-20of-20the-20parasite,-20but-20absent-20in-20bloodstream-20forms-20grown-20-27-27in-20vitro-27-27.-20RNAi-20down-2Dregulation-20of-20TbP5CDH-20severely-20affected-20the-20growth-20of-20procyclic-20trypanosomes-20-27-27in-20vitro-27-27-20in-20the-20absence-20of-20glucose,-20and-20altered-20the-20metabolic-20flux-20when-20proline-20was-20the-20sole-20carbon-20source.-20Furthermore,-20TbP5CDH-20knocked-2Ddown-20cells-20exhibited-20alterations-20in-20the-20mitochondrial-20inner-20membrane-20potential-20(ΔΨm),-20respiratory-20control-20ratio-20and-20ATP-20production.-20Also,-20changes-20in-20the-20proline-2Dglutamate-20oxidative-20capacity-20slightly-20affected-20the-20surface-20expression-20of-20the-20major-20surface-20glycoprotein-20EP-2Dprocyclin.-20In-20the-20tsetse,-20TbP5CDH-20knocked-2Ddown-20cells-20were-20impaired-20and-20thus-20unable-20to-20colonize-20the-20fly's-20midgut,-20probably-20due-20to-20the-20lack-20of-20glucose-20between-20bloodmeals.-20Altogether,-20our-20data-20show-20that-20the-20regulated-20expression-20of-20the-20proline-20metabolism-20pathway-20in-20-27-27T.-20b.-20brucei-27-27-20allows-20this-20parasite-20to-20adapt-20to-20the-20nutritional-20environment-20of-20the-20tsetse-20midgut." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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