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Property "Has-20abstract" (as page type) with input value "Disruption-20of-20cellular-20redox-20homeostasis-20is-20implicated-20in-20a-20wide-20variety-20of-20pathologic-20conditions-20and-20aging.-20A-20fundamental-20factor-20that-20dictates-20such-20balance-20is-20the-20ratio-20between-20mitochondria-2Dmediated-20complete-20oxygen-20reduction-20into-20water-20and-20incomplete-20reduction-20into-20superoxide-20radical-20by-20mitochondria-20and-20NADPH-20oxidase-20(NOX)-20enzymatic-20activity.-20Here-20we-20determined-20mitochondrial-20as-20well-20as-20NOX-2Ddependent-20rates-20of-20oxygen-20consumption-20in-20parallel-20with-20H-3Csub-3E2-3C-2Fsub-3EO-3Csub-3E2-3C-2Fsub-3E-20generation-20in-20freshly-20isolated-20synaptosomes-20using-20high-2Dresolution-20respirometry-20combined-20with-20fluorescence-20or-20electrochemical-20sensory.-20Our-20results-20indicate-20that,-20although-20synaptic-20mitochondria-20exhibit-20substantially-20higher-20respiratory-20activities-20(8-2D82-20folds-20greater-20than-20NOX-20oxygen-20consumption-20depending-20on-20mitochondrial-20respiratory-20state),-20NADPH-2Ddependent-20oxygen-20consumption-20is-20associated-20with-20greater-20H-3Csub-3E2-3C-2Fsub-3EO-3Csub-3E2-3C-2Fsub-3E-20production-20(6-2D7-20folds-20higher-20NOX-2DH-3Csub-3E2-3C-2Fsub-3EO-3Csub-3E2-3C-2Fsub-3E).-20We-20also-20show-20that,-20in-20terms-20of-20the-20consumed-20oxygen,-20while-20synaptic-20mitochondria-20‘leaked’-200.71-25-20±-200.12-20H-3Csub-3E2-3C-2Fsub-3EO-3Csub-3E2-3C-2Fsub-3E-20during-20NAD-3Csup-3E+-3C-2Fsup-3E-2Dlinked-20resting,-200.21-25-20±-200.04-20during-20NAD-3Csup-3E+-3C-2Fsup-3E-2Dlinked-20active,-20and-200.07-25-20±-200.02-20during-20FAD-3Csup-3E+-3C-2Fsup-3E-2Dlinked-20active-20respirations,-20NOX-20converted-2038-25-20±-2013-20of-20O-3Csub-3E2-3C-2Fsub-3E-20into-20H-3Csub-3E2-3C-2Fsub-3EO-3Csub-3E2-3C-2Fsub-3E.-20Our-20results-20indicate-20that-20NOX-20rather-20than-20mitochondria-20is-20the-20major-20source-20of-20synaptic-20H-3Csub-3E2-3C-2Fsub-3EO-3Csub-3E2-3C-2Fsub-3E.-20The-20present-20approach-20may-20assist-20in-20the-20identification-20of-20redox-2Dmodulating-20synaptic-20factors-20that-20underlie-20a-20variety-20of-20physiological-20and-20pathological-20processes-20in-20neurons." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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