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Property "Has-20abstract" (as page type) with input value "Fuel-20substrate-20supply-20and-20oxidative-20phosphorylation-20are-20key-20determinants-20of-20muscle-20performance.-20Numerous-20studies-20of-20mammalian-20mitochondria-20are-20carried-20out-20(i)-20with-20substrate-20supply-20that-20limits-20electron-20flow,-20and-20(ii)-20far-20below-20physiological-20temperature.-20To-20analyze-20potentially-20implicated-20biases,-20we-20studied-20mitochondrial-20respiratory-20control-20in-20permeabilized-20mouse-20myocardial-20fibers-20using-20high-2Dresolution-20respirometry.-20The-20capacity-20of-20oxidative-20phosphorylation-20at-2037-20°C-20was-20nearly-20two-2Dfold-20higher-20when-20fueled-20by-20physiological-20substrate-20combinations-20reconstituting-20tricarboxylic-20acid-20cycle-20function,-20compared-20with-20electron-20flow-20measured-20separately-20through-20NADH-20to-20Complex-20I-20or-20succinate-20to-20Complex-20II.-20The-20relative-20contribution-20of-20the-20NADH-20pathway-20to-20physiological-20respiratory-20capacity-20increased-20with-20a-20decrease-20in-20temperature-20from-2037-20to-2025-20ºC.-20The-20apparent-20excess-20capacity-20of-20cytochrome-20c-20oxidase-20above-20physiological-20pathway-20capacity-20increased-20sharply-20under-20hypothermia-20due-20to-20limitation-20by-20NADH-2Dlinked-20dehydrogenases.-20This-20mechanism-20of-20mitochondrial-20respiratory-20control-20in-20the-20hypothermic-20mammalian-20heart-20is-20comparable-20to-20the-20pattern-20in-20ectotherm-20species,-20pointing-20towards-20NADH-2Dlinked-20mt-2Dmatrix-20dehydrogenases-20and-20the-20phosphorylation-20system-20rather-20than-20electron-20transfer-20complexes-20as-20the-20primary-20drivers-20of-20thermal-20sensitivity-20at-20low-20temperature.-20Delineating-20the-20link-20between-20stress-20and-20remodeling-20of-20oxidative-20phosphorylation-20is-20important-20for-20understanding-20metabolic-20perturbations-20in-20disease-20evolution-20and-20cardiac-20protection." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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