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Property "Has-20abstract" (as page type) with input value "In-20a-20comparative-20study,-20we-20analysed-20the-20effects-20of-20adenosine-20on-20the-20energetics,-20protein-20synthesis-20and-20K(+-20)homeostasis-20of-20hepatocytes-20from-20the-20anoxia-2Dtolerant-20goldfish-20Carassius-20auratus-20and-20the-20anoxia-2Dintolerant-20trout-20Oncorhynchus-20mykiss.-20The-20rate-20of-20oxygen-20consumption-20did-20not-20respond-20immediately-20to-20the-20addition-20of-20adenosine-20to-20the-20cells-20from-20either-20species,-20but-20showed-20a-20significant-20decrease-20in-20trout-20hepatocytes-20after-2030-20min.-20The-20anaerobic-20rate-20of-20lactate-20formation-20was-20not-20significantly-20affected-20by-20adenosine-20in-20goldfish-20hepatocytes,-20but-20was-20increased-20in-20trout-20cells.-20We-20also-20studied-20the-20effects-20of-20adenosine-20on-20the-20two-20most-20prominent-20ATP-20consumers-20in-20these-20cells,-20protein-20synthesis-20and-20Na(+)-2FK(+)-2DATPase-20activity.-20Under-20aerobic-20conditions,-20adenosine-20inhibited-20protein-20synthesis-20of-20hepatocytes-20from-20goldfish-20by-2051-25-20and-20of-20hepatocytes-20from-20trout-20by-2032-25.-20During-20anoxia,-20the-20rate-20of-20protein-20synthesis-20decreased-20by-20approximately-2050-25-20in-20goldfish-20hepatocytes-20and-20by-2090-25-20in-20trout-20hepatocytes,-20and-20this-20decrease-20was-20not-20altered-20by-20the-20presence-20of-20adenosine.-20Adenosine-20inhibited-20normoxic-20Na(+)-2FK(+)-2DATPase-20activity-20and-20K(+-20)efflux-20by-2020-2D35-25-20in-20the-20cells-20of-20both-20species.-20An-20investigation-20into-20the-20mechanism-20underlying-20the-20inhibition-20of-20protein-20synthesis-20by-20adenosine-20indicated-20that,-20in-20the-20goldfish-20cells,-20adenosine-20acts-20via-20a-20membrane-20receptor-2Dmediated-20pathway,-20i.e.-20the-20effect-20of-20adenosine-20was-20abolished-20by-20applying-20the-20A1-20receptor-20antagonist-208-2Dphenyltheophylline.-20In-20the-20trout,-20however,-20the-20uptake-20of-20adenosine-20into-20hepatocytes-20seems-20to-20be-20required-20for-20an-20effect-20on-20protein-20synthesis.-20-5BCa(2+)-5D(i)-20does-20not-20seem-20to-20be-20involved-20in-20the-20inhibition-20of-20protein-20synthesis-20by-20adenosine" contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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