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Property "Has-20abstract" (as page type) with input value "Remote-20ischemic-20preconditioning-20(RIP)-2Dinduced-20protection-20of-20myocardial-20energetics-20was-20well-20documented-20on-20the-20level-20of-20tissue,-20but-20data-20concerning-20the-20involvement-20of-20mitochondria-20were-20missing.-20We-20aimed-20at-20the-20identification-20of-20changes-20in-20membrane-20properties-20and-20respiratory-20functions-20induced-20in-20rat-20heart-20mitochondria-20by-20RIP.-20Experiments-20were-20performed-20on-2046-20male-20Wistar-20rats-20spanided-20into-20control-20and-20RIP-2Dtreated-20groups-20of-2021-20animals-20each.-20Blood-20flow-20in-20the-20occluded-20area-20was-20recorded-20by-20MRI-20angiography-20in-20four-20animals.-20RIP-20protocol-20comprised-20of-20three-20successive-205-2Dmin-20occlusions-20each-20followed-20by-205-2Dmin-20reperfusions-20of-20descending-20branches-20of-20the-20right-20hind-20limb-20femoral-20artery.-20The-20efficacy-20of-20RIP-20was-20evaluated-20as-20the-20extent-20of-20RIP-2Dinduced-20protection-20against-20damage-20to-20the-20functions-20of-20mitochondria-20isolated-20by-20differential-20centrifugation-20after-2030-2Dmin-20global-20ischemia-20followed-20by-2040-2Dmin-20reperfusion-20of-20the-20hearts-20in-20Langendorff-20mode.-0A-0AAssessments:-20Mitochondrial-20membrane-20fluidity-20with-20a-20fluorescent-20probe-20DPH,-20CoQ(9)-20and-20CoQ(10)-20with-20HPLC,-20mitochondrial-20respiration-20with-20the-20Oxygraph-2D2k-20(Oroboros).-20Results-20revealed-20that-20RIP-20was-20affecting-20the-20mitochondria.-20The-20immediate-20protection-20conferred-20by-20RIP-20involves-20beneficial-20and-20prognostically-20significant-20effects:-20a-20total-20elimination-20of-20ischemia-2Freperfusion-2Dinduced-20depression-20of-20mitochondrial-20membrane-20fluidity-20and-20a-20trend-20for-20better-20preservation-20of-20mitochondrial-20state-203-20respiration." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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