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Property "Has-20abstract" (as page type) with input value "Tumor-20cells-20are-20subjected-20to-20a-20broad-20range-20of-20selective-20pressures.-20As-20a-20result-20of-20the-20imposed-20stress,-20subpopulations-20of-20surviving-20cells-20exhibit-20inspanidual-20biochemical-20phenotypes-20that-20reflect-20metabolic-20reprograming.-20The-20present-20work-20aimed-20at-20investigating-20metabolic-20parameters-20of-20cells-20displaying-20increasing-20degrees-20of-20metastatic-20potential.-20The-20metabolites-20present-20in-20cell-20extracts-20fraction-20of-20tongue-20fibroblasts-20and-20of-20cell-20lines-20derived-20from-20human-20tongue-20squamous-20cell-20carcinoma-20lineages-20displaying-20increasing-20metastatic-20potential-20(SCC9-20ZsG,-20LN1-20and-20LN2)-20were-20analyzed-20by-20-3Csup-3E1-3C-2Fsup-3EH-20NMR-20(nuclear-20magnetic-20resonance)-20spectroscopy.-20Living,-20intact-20cells-20were-20also-20examined-20by-20the-20non-2Dinvasive-20method-20of-20fluorescence-20lifetime-20imaging-20microscopy-20(FLIM)-20based-20on-20the-20auto-20fluorescence-20of-20endogenous-20NADH.-20The-20cell-20lines-20reproducibly-20exhibited-20distinct-20metabolic-20profiles-20confirmed-20by-20Partial-20Least-2DSquare-20Discriminant-20Analysis-20(PLS-2DDA)-20of-20the-20spectra.-20Measurement-20of-20endogenous-20free-20and-20bound-20NAD(P)H-20relative-20concentrations-20in-20the-20intact-20cell-20lines-20showed-20that-20ZsG-20and-20LN1-20cells-20displayed-20high-20heterogeneity-20in-20the-20energy-20metabolism,-20indicating-20that-20the-20cells-20would-20oscillate-20between-20glycolysis-20and-20oxidative-20metabolism-20depending-20on-20the-20microenvironment's-20composition.-20However,-20LN2-20cells-20appeared-20to-20have-20more-20contributions-20to-20the-20oxidative-20status,-20displaying-20a-20lower-20NAD(P)H-20free-2Fbound-20ratio.-20Functional-20experiments-20of-20energy-20metabolism,-20mitochondrial-20physiology,-20and-20proliferation-20assays-20revealed-20that-20all-20lineages-20exhibited-20similar-20energy-20features,-20although-20resorting-20to-20different-20bioenergetics-20strategies-20to-20face-20metabolic-20demands.-20These-20differentiated-20functions-20may-20also-20promote-20metastasis.-20We-20propose-20that-20lipid-20metabolism-20is-20related-20to-20the-20increased-20invasiveness-20as-20a-20result-20of-20the-20accumulation-20of-20malonate,-20methyl-20malonic-20acid,-20n-2Dacetyl-20and-20unsaturated-20fatty-20acids-20(CH-3Csub-3E2-3C-2Fsub-3E)-3Csub-3En-3C-2Fsub-3E-20in-20parallel-20with-20the-20metastatic-20potential-20progression,-20thus-20suggesting-20that-20the-20NAD(P)H-20reflected-20the-20lipid-20catabolic-2Fanabolic-20pathways." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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