Difference between revisions of "Spectrofluorometer"
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|description=A '''spectrofluorometer''' makes use of a [[ | |description=A '''spectrofluorometer''' makes use of a [[spectrometer]] to measure and analyse the fluorescent emission spectra from a [[fluorophore]]. It will typically differ from an [[absorbance]] [[spectrophotometer]] in that it will have a larger [[slit width]] (to increase [[sensitivity]]) and use a longer [[integration time]]. The configuration of the illuminating and receiving optics also differ from [[spectrophotometry]] in that the excitation source is directed perpendicularly to the position of the emission [[detector]] so that the intensity of the excitation signal reaching the [[detector]] is minimised. | ||
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{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry | |mitopedia method=Fluorometry | ||
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{{MitoPedia | {{MitoPedia O2k and high-resolution respirometry}} | ||
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Latest revision as of 14:36, 8 February 2016
- high-resolution terminology - matching measurements at high-resolution
Spectrofluorometer
Description
A spectrofluorometer makes use of a spectrometer to measure and analyse the fluorescent emission spectra from a fluorophore. It will typically differ from an absorbance spectrophotometer in that it will have a larger slit width (to increase sensitivity) and use a longer integration time. The configuration of the illuminating and receiving optics also differ from spectrophotometry in that the excitation source is directed perpendicularly to the position of the emission detector so that the intensity of the excitation signal reaching the detector is minimised.
MitoPedia methods: Fluorometry
