Difference between revisions of "Spectrofluorometer"
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|description=A '''spectrofluorometer''' makes use of a [[spectrophotometer]] to measure and analyse the fluorescent emission spectra from [[ | |description=A '''spectrofluorometer''' makes use of a [[spectrophotometer]] to measure and analyse the fluorescent emission spectra from a [[fluorophore]]. It will typically differ from an [[absorbance]] [[spectrophotometer]] in that it will have a larger [[slit width]] (to increase [[sensitivity]]) and use a longer [[integration time]]. The configuration of the illuminating and receiving optics also differ from [[spectrophotometry]] in that the excitation source is directed perpendicularly to the position of the emission [[detector]] so that the intensity of the excitation signal reaching the [[detector]] is minimised. | ||
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{{MitoPedia methods | {{MitoPedia methods | ||
Revision as of 17:15, 7 December 2011
- high-resolution terminology - matching measurements at high-resolution
Spectrofluorometer
Description
A spectrofluorometer makes use of a spectrophotometer to measure and analyse the fluorescent emission spectra from a fluorophore. It will typically differ from an absorbance spectrophotometer in that it will have a larger slit width (to increase sensitivity) and use a longer integration time. The configuration of the illuminating and receiving optics also differ from spectrophotometry in that the excitation source is directed perpendicularly to the position of the emission detector so that the intensity of the excitation signal reaching the detector is minimised.
Abbreviation: n.a.
MitoPedia methods:
Fluorometry,
Spectrophotometry
