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Difference between revisions of "Succinate"

From Bioblast
(replaced FW by M and added units g*mol-1)
(moved comments from the titration section to the comments section)
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:::'''S: Succinate''' (Succinate disodium salt, hexahydrate, C<sub>4</sub>H<sub>4</sub>0<sub>4</sub>Na<sub>2</sub> * (H<sub>2</sub>O)<sub>6</sub>; Sigma S 2378, 100 g, store at RT; M = 270.1 g·mol<sup>-1</sup>)
:::'''S: Succinate''' (Succinate disodium salt, hexahydrate, C<sub>4</sub>H<sub>4</sub>0<sub>4</sub>Na<sub>2</sub> * (H<sub>2</sub>O)<sub>6</sub>; Sigma S 2378, 100 g, store at RT; M = 270.1 g·mol<sup>-1</sup>)
:::: In the absence of CI-linked substrates, add the CI-inhibitor [[rotenone]] before addition of succinate, to avoid accumulation of [[oxaloacetate]] with subsequent inhibition of [[succinate dehydrogenase]]. See: [[Succinate and rotenone]].
:::: When keeping the succinate stock solution on ice, check for complete solubilization of succinate and warm the stock solution in your hands if necessary.




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:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]]  
:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]]  


::::* In the absence of CI-linked substrates, add the CI-inhibitor [[rotenone]] before addition of succinate, to avoid accumulation of [[oxaloacetate]] with subsequent inhibition of [[succinate dehydrogenase]]. See: [[Succinate and rotenone]].
::::* When keeping the succinate stock solution on ice, check for complete solubilization of succinate and warm the stock solution in your hands if necessary.
::::* Titration volume ('''2-mL O2k-chamber'''): 20 µL using a 50 µL Hamilton syringe.
::::* Titration volume ('''2-mL O2k-chamber'''): 20 µL using a 50 µL Hamilton syringe.
::::* Titration volume ('''0.5-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe
::::* Titration volume ('''0.5-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe

Revision as of 08:48, 16 March 2021


high-resolution terminology - matching measurements at high-resolution


Succinate

Description

Succinic acid

Succinic acid, C4H6O4, (butanedioic acid) is a dicarboxylic acid which occurs under physiological conditions as the anion succinate2-, S, with pKa1 = 4.2 and pKa2 = 5.6. Succinate is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction. Succinate (CII-linked) and NADH (CI-linked) provide convergent electron entries into the Q-junction. Succinate is transported across the inner mt-membrane by the dicarboxylate carrier. The plasma membrane of many cell types is impermeable for succinate (but see Zhunussova 2015 Am J Cancer Res for an exception). Incubation of mt-preparations by succinate alone may lead to accumulation of oxaloacetate, which is a potent inhibitor of Complex II (compare Succinate and rotenone). High activities of mt-Malic enzyme (mtME) prevent accumulation of oxaloacetate.

Abbreviation: S

Reference: Gnaiger 2019 MitoPathways, Tretter 2016 Biochim Biophys Acta

Succinate, S. From Gnaiger 2014 MitoPathways.

Application in HRR

S: Succinate (Succinate disodium salt, hexahydrate, C4H404Na2 * (H2O)6; Sigma S 2378, 100 g, store at RT; M = 270.1 g·mol-1)
In the absence of CI-linked substrates, add the CI-inhibitor rotenone before addition of succinate, to avoid accumulation of oxaloacetate with subsequent inhibition of succinate dehydrogenase. See: Succinate and rotenone.
When keeping the succinate stock solution on ice, check for complete solubilization of succinate and warm the stock solution in your hands if necessary.


Preparation of 1 M stock solution:
  1. Weight 1.3505 g of succinate and dissolve in 3 mL H2O;
  2. Check pH and adjust to 7.0 if necessary with 1 M HCl (about 65 µL will be needed);
  3. Transfer to 5 mL volumetric glass flask and adjust the final volume to 5 mL;
  4. Divide into 0.5 mL portions;
  5. Store frozen at -20 °C.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
  • Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe
  • Final concentration: 10 mM. Note: The concentration of succinate may be increased up to 50 mM after rotenone to compensate for the inhibitory effect of malate.


SUITbrowser question: Succinate pathway

The SUITbrowser can be used to find the best SUIT protocols to analyze the succinate pathway, among other research questions.


MitoPedia topics: Substrate and metabolite