Difference between revisions of "Superoxide dismutase"

Revision as of 15:49, 20 October 2016

high-resolution terminology - matching measurements at high-resolution

Superoxide dismutase

Description

Mammalian superoxide dismutase (SOD) exists in three forms, of which the Mn-SOD occurs in mitochondria (mtSOD, SOD2; 93 kD homotetramer) and many bacteria, in contrast to the Cu-Zn forms of SOD (cytosolic SOD1, extracellular SOD3 anchored to the extracellular matrix and cell surface). Superoxide anion (O₂^•-) is a major reactive oxygen species (ROS) which is dismutated by SOD to oxygen and H₂O₂.

Abbreviation: SOD

Reference: Fridovich_1997_J Biol Chem

MitoPedia topics: Enzyme

Application in fluorometry

Add SOD for conversion of O₂^•- and measurement of H₂O₂ with Amplex Red.

SOD: Superoxide Dismutase from bovine liver, Sigma S8409, 15 KU, store at -20°C; MW = 32.000,00 g/mol

Preparation of 5000U/ml stock solution (dissolved in H₂O):

1) Weigh 2 mg of Superoxide dismutase

2) Dissolve in 1134µl H₂O

3) Divide into 30µl portions into 0.2 ml eppendorf tubes

4) Store frozen at -20°C in the Desiccator

@@ Line 11: / Line 11: @@
 == Application in fluorometry  ==
 * Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[MiPNet20.14_AmplexRed_H2O2-production|measurement of H<sub>2</sub>O<sub>2</sub> with Amplex Red]].
+'''SOD:  Superoxide Dismutase from bovine liver''', Sigma S8409, 15 KU, store at -20°C; MW = 32.000,00 g/mol
+'''Preparation of 5000U/ml stock solution''' (dissolved in H<sub>2</sub>O):
+::1) Weigh 2 mg of Superoxide dismutase
+::2) Dissolve in 1134µl H<sub>2</sub>O
+::3) Divide into 30µl portions into 0.2 ml eppendorf tubes
+::4) Store frozen at -20°C in the Desiccator