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TIP2k-Module

From Bioblast

                



TIP2k-Module

O2k-Catalogue

Description TIP2k-Module - Titration-Injection microPump (TIP2k) for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat). The TIP2k-Module includes TIP2k-Accessories: 4 Microinjection syringes, 1 Pkg. TIP2k-Needle Spacers with mounting tool, 1 TIP2k-Needle Safety Support and 1 Pkg. TIP2k Filter Papers, and the newest DatLab version.
Product ID 11100-03
Type O2k, O2k-Module, Microsyringe, O2k-FluoRespirometer
Link MiPNet12.10 TIP2k-manual, MiPNet04.05 Titration-Injection
Image
TIP2k with 200 mm3 microsyringe.JPG


MitoPedia O2k and high-resolution respirometry: O2k hardware 

TIP2k-Module consists of:

O2k with TIP2k

» TIP2k-Manual
The TIP2k is fully supported by the O2k.

The TIP2k is placed at the top of the Oroboros O2k. It does not need any extra bench space.

  • Align the front legs of the TIP2k with the TIP2k-Spacer Bar on the front of the O2k if you use 200 µl syringes.
  • Align the rear ends of the TIP2k and O2k, if you use 500 µl syringes.

Technical characteristics

Titration: Programmable, automatic titration regimes, with titration volumes of 0.05 to 250 µl, variable titration intervals and duration of titration pulses.

Injection: Steady-state injection: Operation at quasi steady-states by continuous injection of substrates at limiting rates of consumption, providing new flexibility in experimental design by combining the technical advantages of closed and open systems. Programmable injection flows: 0.01 to 25 µl.s-1. Ramp injection (MiPNet04.05, see above): Ramp increase of effector concentrations by "continuous titration".

  • DatLab software for feedback control by the the TIP2k: for steady-state respirometry at selected oxygen levels and pH-stat applications.

TIP2k-Syringes

» SOP: Handling microsyringes
  • Rinse the outside with water immediately after use.
  • Wash 3 x with last used solvent, rinse with EtOH.
  • Storage: ethanol (short), dry (longtime).
  • Rinse with pure solvent before use.


List of O2k-Publications: TIP2k