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| '''Fragile samples'''
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| Unfortunately, the rigorous stirring required for high- resolution respirometry causes problems for organisms / samples that are easily damaged by mechanical force, such as fish eggs or beads used to grow adherent cells. Therefore the [[Adherent cells]] problem in the oxygraph is linked to the fragile sample problem.
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| One possible work around for fragile samples (including large beads) is to construct a cage from wire that holds the actual sample. A few threads of this wire cage can be used to position the cage inside the chamber by holding it against the chamber walls. This requires samples large enough to be retained by a wire cage wide-meshed enough to allow for sufficient stirring of the liquid.
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| However, this and other methods are always a compromise. Experiences with these or other methods would be extremely welcome on this page and on its accompanying Discussion page!
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| {{#set:Technical service=Chamber}}
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| __SHOWFACTBOX__
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| [[Category:Technical service]]
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| == From the correspondence ==
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| Question: Would smaller stirring bars help to minimize damage (combined with lower stirring speed)? Would they be available?
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| I am not sure that smaller stirring bars would help to avoid destruction. In the early phase of the development of the oxygraph many different geometries and sizes of stirring bars were explored. I fear that producing special small stirring bars will result in a prohibitive price, especially if it is not clear whether there is any positive effect of sample stability.
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| Smaller commercially available stirring bars cannot be used for high-resolution respirometry due to their Teflon (R) coating, but could you test whether they reduce the mechanical damage to your sample at a reasonable high stirring speed?
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| Reducing reducing stirring speed will always severely compromise sensitivity and response time.
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| [[User:Fasching Mario|Fasching Mario]]
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