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Difference between revisions of "Talk:O2k-Open Support"

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= Overview =
[[File:MitoPedia.jpg|left|100px|MitoPedia|link=MitoPedia]]
== MitoPedia ==
:::: Find information quickly with '''[[MitoPedia|MitoPedia]]''', the Bioblast glossary with lists of key words and links to relevant pages, specifically and the O2k and high-resolution respirometry:Β  Β  Β 
<br/>
::::Β» [[MitoPedia:_O2k_hardware]]
::::Β» [[MitoPedia:_DatLab]]
::::Β» [[MitoPedia:_SUIT]]: Substrate-uncoupler-inhibitor-titration protocolsΒ  Β  Β 
[[File:O2k-Manual.jpg|left|100px|O2k-Manual|link=http://wiki.oroboros.at/index.php/O2k-Manual]]
== O2k-Manual ==
::::The '''[[O2k-Manual|O2k-Manual]]''' helps beginners and experienced users. Context sensitive help is provided in the DatLab software: '''Β» [[MitoPedia:_DatLab]]'''Β  Β  Β 
::::Β» Polarographic oxygen sensors: calibration, accuracy and quality control SOP Β» [[MiPNet06.03_POS-calibration-SOP|MiPNet06.03 POS-calibration-SOP]]
::::Β» Instrumental O<sub>2</sub> background correction, accuracy of oxygen flux and SOP Β» [[MiPNet14.06_Instrumental_O2_background|MiPNet14.06 Instrumental O2 background]]Β  Β  Β 
<br/>
[[File:PlayVideo.jpg|left|100px|O2k-Videosupport|link=http://wiki.oroboros.at/index.php/O2k-Videosupport]]
== O2k-Videosupport ==
::::Β» '''[[O2k-Videosupport|O2k-Videosupport]]'''Β  Β  Β 
[[File:O2k-Protocols.jpg|left|100px|O2k-Procedures|link=http://wiki.oroboros.at/index.php/O2k-Procedures]]
== O2k-Procedures ==
::::'''O2k-Procedures''' explain various applications of the O2k with O2k-Demo experiments, and more: '''Β» [[O2k-Procedures]]'''Β  Β  Β 
{| class="wikitable" border="1"
|-
| [[File:O2k-Protocols.jpg|left|60px|O2k-Procedures]]
| <big>Β» [[O2k-Procedures:_MitoPathways]]</big>
|-
| [[File:Pesta 2012 Methods Mol Biol Fig7A.jpg|left|60px|Pesta 2012 Methods Mol Biol Fig7A.jpg|link=Pesta 2012 Methods Mol Biol/SUIT-Protocol]]
| <big>Β» [[O2k-Demo_experiments|O2k-Demo experiments]]</big>
|-
| [[File:O2k-Protocols.jpg|left|60px|O2k-Procedures]]
| <big>Β» [[O2k-Chemicals_and_media|O2k-Chemicals and media]]</big>
|-
| [[File:PBI-Shredder HRR-Set.JPG|left|30px|PBI-Shredder O2k-Set|link=PBI-Shredder O2k-Set]]
| <big>Β» [[O2k-mitochondrial_preparations|O2k-mitochondrial preparations]]</big>
|-
| [[File:O2k-Protocols.jpg|left|60px|O2k-Procedures]]
| <big>Β» [[O2k-Procedures:_Mitochondrial_and_marker-enzymes]]</big>
|}
::::* Implement your quality control management with the [[MitoFit_Quality_Control_System|MitoFit QCS]], to diagnose technical problems fast and cost-effectively with instrumental quality control tests.Β 
[[File:Questions.jpg|left|50px|Questions.jpg]]
== Frequently Asked Questions ==
::::Β» '''[[Oroboros_FAQ|Oroboros FAQ]]'''Β  Β  Β 
= Technical service - pages in preparation =
::::* Introduction to O2k-technical support: [[Media:Technical Support.pdf|'''Β»O2k-technical support.pdf''']]
::::* The pages are listed in the [http://wiki.oroboros.at/index.php/Category:Technical_service Browse data page], starting with "Talk:.."
::::* [[MiPNet14.06 Instrumental O2 background]] - Flux at closed chamber near to air saturation


== Technical service by telephone ==
== Technical service by telephone ==
::::We prefer to give technical assistance (β€žtrouble shootingβ€œ) via Email. We are aware that, especially if there is an urgent problem, some end-users would prefer to communicate by telephone. However, we are convinced that there are very good reasons for our approach:
::::O2k-Open Support is provided via e-mail. We are aware that, especially if there is an urgent problem, some end-users would prefer to communicate by telephone. However, that there are very good reasons for our approach:


:::Communication by Email Β 
:::Communication by e-mail Β 
::::* gives us the time to thoroughly read the customers problem description, analyze in detail the files sent to us and THINK about our proposals BEFORE suggesting them to you, Β 
::::* gives us the time to thoroughly read the customers problem description, analyze in detail the files sent to us and THINK about our proposals BEFORE suggesting them to you. Β 
::::* gives the customer time to read our suggestions carefully and to read any additional material (websites , manual !) that we proposed,
::::* gives the customer time to read our suggestions carefully and to read any additional material (websites , manual !) that we proposed.
::::* gives the customer time to properly collect (and document) the data that we asked her/him to send, Β 
::::* gives the customer time to properly collect (and document) the data that we asked her/him to send. Β 
::::* allows our technical service to be operated "by scientists, for scientists". The members of our technical service team work on their own project, keeping up to date with all technical development. However, this means they will not be available for phone conversation while running their experiments.
::::* allows our technical service to be operated "by scientists, for scientists". The members of our technical service team work on their own project, keeping up to date with all technical development. However, this means they will not be available for phone conversation while running their experiments.
::::For a few special cases telephone conversations may actually make sense. In this case we will of course gladly arrange for such aΒ  phone call (planned beforehand).
::::For a few special cases telephone conversations may actually make sense. In this rare cases (when all the troubleshooting according to our SOPs provided by the customer do not yield a solution or understanding of the problem) we will gladly arrange for such aΒ  phone call (planned beforehand).


::::When phoning Oroboros Instruments you will be connected to our administrative team. They will be glad to help you in administrative questions and point you to the right starting places for trouble shooting. Please understand that it will not be helpful to describe to them any technical details related to a problem on the phone. Even our technical staff prefer to get this information by e-mail, information relayed via non technical experts would be even less helpful. Therefore, this information should be sent by e-mail and include as much of the information gathered during trouble shooting (see the main page) as possible, including a DatLab file (not aΒ  screen shot) ofΒ  an [[Oxygen sensor test]].
::::When phoning Oroboros Instruments you will be connected to our administrative team. They will be glad to help you in administrative questions and point you to the right starting places for trouble shooting. Please understand that it will not be helpful to describe to them any technical details related to a problem on the phone. Even our technical staff prefer to get this information by e-mail, information relayed via non technical experts would be even less helpful. Therefore, this information should be sent by e-mail and include as much of the information gathered during trouble shooting (see the main page) as possible, including a DatLab file (not aΒ  screen shot) ofΒ  an [[Oxygen sensor test]].


::::[[User:Fasching Mario|Fasching Mario]] 09:35, 17 April 2013 (CEST)
::::[[User:Fasching Mario|Fasching Mario]] 09:35, 17 April 2013 (CEST)


[[Image:BB-Bioblast.jpg|left|30px|link=http://www.bioblast.at/index.php/Bioblast:About |Bioblast wiki]]
[[Image:BB-Bioblast.jpg|left|30px|link=http://www.bioblast.at/index.php/Bioblast:About |Bioblast wiki]]
== Popular Bioblast page ==
== Popular Bioblast page ==
:::[[O2k-technical support and open innovation]] has been accessed more than Β 
:::[[O2k-Open Support]] has been accessed more than Β 
::::* 70,000 times (2019-12-11)
::::* 60,000 times (2019-07-22)
::::* 50,000 times (2018-10-18)
::::* 50,000 times (2018-10-18)
::::* 40,000 times (2017-01-24)
::::* 40,000 times (2017-01-24)
Line 107: Line 29:
::::* 10,000 times (2014-04-17)
::::* 10,000 times (2014-04-17)
::::*Β  5,000 times (2012-06-12)
::::*Β  5,000 times (2012-06-12)
== Different Temperature Settings Effects on initial O2 concentration in Chambers ==
I work with different temperature settings, e.g., 20, 30 37 celsius.
Should I expect different initial O2 concentrations in chambers, to begin with?
== The most frequent problems reported by customers ==
Most problems can be readily solved when consulting the '''[[O2k-Manual| O2k-Manual]]''' of the O2k. We thus ask our customers to see the relevant chapter of the Manual before contacting the customer support.
'''Sensor signal is not stable'''
::::1. Check if you ran through the [[O2_calibration_-_DatLab#Calibration_and_quality_control | SOPs for Calibration and quality control]]
'''One chamber works well, the other does not'''
:::: Locating the problem:
::::1. If the problem persists, try to locate the problem running the following experiment, while permanently recording:
::::2. Fill the chambers with medium or water and [[O2_calibration_-_DatLab#Air_calibration_-_general_description | air calibrate]]
::::3. Perform a [[Stirrer_test | stirrer test]].
::::4. When the signal is stable, empty the chambers (keep recording!), mark the [[Event | Event]] and describe it in the Event window
::::5. Remove the POS-sensors from the chambers, unscrew the OroboPOS from the Connectors and swap the POS-sensors between chambers
::::6. Insert the Connector-attached POS into chambers again, refill the chambers with pre-warmed medium, mark and denote the Event (e.g. Sensors swapped)
::::7. Let this run for a few minutes, then again empty the chambers, remove sensors from chambers, unscrew the OroboPOS from the Connectors and swap the POS-connectors between chambers (so you have the original combination of POS and Connector but at the other chamber)
::::8. Refill the chambers with pre-warmed medium, mark and denote the Event (e.g. Connectors swapped)
::::9. Let this run for a few minutes and then save the file (e.g. Swap components.DLD).
If you find that the problem follows the OroboPOS, this typically indicates that a [[MiPNet19.18B_POS-service | POS-Service]] is required. In some case, a repeated POS-Service may be needed.
'''Dry some components:'''
In some cases, water (excessive spillage, high humidity) is the problem, causing problems at electrical connections.
::::1. Remove the [[OroboPOS | OroboPOS]] with [[OroboPOS-Connector | Connectors]] from the chamber
::::2. Unscrew the OroboPOS from the Connector
::::3. Put the Connector to 50Β°C for 2h for drying.
::::4. Gently dry the connecting part of the OroboPOS
::::5. (Application of a drop of contact oil (as previously recommended) is generally not required, but when done make sure only very little oil is applied to each of the golden pins and threads on POS connector and POS.)
::::6. Dry the socket where the Connector is plugged into the O2k, using a hair dryer or similar device.
'''Zero voltage is too high'''
::::During [[Zero_calibration | Zero calibration]] (setting R0) the sensor signal (in Volt) at zero oxygen concentration is determined. If the signal does not decline rapidly to between 0% and 5% of the signal seen at air saturation then:
::::1. check your [[Dithionite | Dithionite]] powder. Dithionite may deteriorate over time. Thus, if even adding more does not help it must be renewed.
::::2. perform a POS-Service (sometimes a repeated service is required).
After performing the sensor service, mounting a new membrane and time for the sensor to stabilize, typical raw signals (in V) determined at air equilibration can be seen here: http://bioblast.at/index.php/Raw_signal
'''The glass chamber is dirty or appears opaque or the stirrer bar is jumping'''
:::[http://wiki.oroboros.at/index.php/O2k-Chamber_MitoPedia#Cleaning_the_glass_chamber Cleaning the glass chamber]
:::[https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be Chamber assembly]
'''Different signals between chambers'''
:::http://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP
:::http://wiki.oroboros.at/index.php/O2_calibration_-_DatLab
:::http://wiki.oroboros.at/index.php/Air_calibration
:::http://wiki.oroboros.at/index.php/Zero_calibration
http://wiki.oroboros.at/index.php/MiPNet19.18B_POS-service
:::http://wiki.oroboros.at/index.php/Instrumental_background_oxygen_flux

Latest revision as of 09:20, 6 March 2020


Technical service by telephone

O2k-Open Support is provided via e-mail. We are aware that, especially if there is an urgent problem, some end-users would prefer to communicate by telephone. However, that there are very good reasons for our approach:
Communication by e-mail
  • gives us the time to thoroughly read the customers problem description, analyze in detail the files sent to us and THINK about our proposals BEFORE suggesting them to you.
  • gives the customer time to read our suggestions carefully and to read any additional material (websites , manual !) that we proposed.
  • gives the customer time to properly collect (and document) the data that we asked her/him to send.
  • allows our technical service to be operated "by scientists, for scientists". The members of our technical service team work on their own project, keeping up to date with all technical development. However, this means they will not be available for phone conversation while running their experiments.
For a few special cases telephone conversations may actually make sense. In this rare cases (when all the troubleshooting according to our SOPs provided by the customer do not yield a solution or understanding of the problem) we will gladly arrange for such a phone call (planned beforehand).
When phoning Oroboros Instruments you will be connected to our administrative team. They will be glad to help you in administrative questions and point you to the right starting places for trouble shooting. Please understand that it will not be helpful to describe to them any technical details related to a problem on the phone. Even our technical staff prefer to get this information by e-mail, information relayed via non technical experts would be even less helpful. Therefore, this information should be sent by e-mail and include as much of the information gathered during trouble shooting (see the main page) as possible, including a DatLab file (not a screen shot) of an Oxygen sensor test.
Fasching Mario 09:35, 17 April 2013 (CEST)
Bioblast wiki

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