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Talk:O2k-Peltier Temperature Control

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O2k-Open Support

Talk:O2k-Peltier Temperature Control



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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Very low temperatures

Question: Is it possible to use the oxygraph with a chamber temperature of 0 °C?

Our system is specified for a chamber temperature down to 4 °C at 25 °C room temperature and down to 2 °C at "lower ambient temperature", see specifications. Lower ambient temperature does not necessarily mean a "cool room". For a chamber temperature of 2 °C, an air conditioned room will usually suffice. While we can only guarantee the working of the instrument within these limits, it is possible to reach a chamber temperature of 0 °C at a "lower ambient temperature". In general, however, this may require a "cool room". For this purpose, the main unit itself can be exposed to ambient temperatures down to just above 0°C (all the electronic components are rated at least down to 0 °C, but 0 °C room temperature should be avoided due to condensation concerns). Also, for very low room temperatures above 0 °C, some means of humidity control should be used. We recently performed a short test and were able to reach 0 °C chamber temperature quite easily at a room temperature of about 14 °C. In order to maintain this temperature, the cooling power of the Peltier element reached 95% of maximum. Thus, a "cool room" would probably be necessary for longer experiments.

Update for O2k Series E: Because Series E Oxygraphs have a more efficient temperature management system the experiment described above was repeated with a Series E O2k. In this case it was not necessary to reduce the room temperature. Fasching Mario 14:39, 4 February 2013 (CET)

Details: O2K - 0°C block temp. - stable Peltier power was reached after about 1:05 h from setting block temp. from stable 37°C to 0°C. - stable Peltier power was about -83 %. - room temperature was 21.1°C Capek Ondrej 14:36, 4 February 2013 (CET)