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Difference between revisions of "Talk:O2k-TPP+ ISE-Module"

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== Other ions beside TPP+ TPMP+ , and Ca2+ ==
Is only Ca+2 is detected with the suitable module? Or, other ions such Na+, phosphate, K+ can also be detected?
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'''Answer:'''
We offer membranes to detect TPP+ and Ca2+. There are ion selective membranes available e.g. from Sigma Aldrich for a wide variety of species. If cut to a circle with a diameter of 4 mm these membranes can be used in our ISE system.
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Many ions may also be detected by fluorimetric based methods, see [[http://wiki.oroboros.at/index.php/O2k-Fluorescence_LED2-Module]]
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--[[User:Fasching Mario|Fasching Mario]] 09:48, 22 February 2012 (CET)
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== Types of connectors ==
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'''Question:''' Are the ISE sockets a BNC cable connection?
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'''Answer:''' In Series D and higher the ISE connectors consists of 2 ports: 1.) a BNC port: both poles are "live" so it can be used either for aย  combination electrode (like a classical pH) or for just one singeย  electrode that requires a separate reference electrode 2.) a 2 mm pin type connection that is used for connecting the separate reference electrodes we use for TPP and now also for pH This 2 mm pin port connects to the same electronics as the outer channel of the BNC port. See also chapter 3.2 of the ISE Manual [http://www.oroboros.at/index.php?id=o2k-multisensor-manual MiPNet15.03]. In the same manual you find the relevant information for older series of the oxygraph.[[User:Fasching Mario|Fasching Mario]] 09:22, 19 September 2012 (CEST)
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== Long term storage of the TPP electrode ==
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Question: What is the procedure for long term storage of the TPP electrode?
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Answer: For long time storage (several months) please disassemble the electrode,ย  wash all parts with ethanol (sterilization), allow all parts to becomeย  completely dry, then re-assemble the dry electrode without a membrane(toย  have everything together and to avoid loosingย  small parts). Then storeย  the electrode in a dark place like the TPP accessory box. [[User:Fasching Mario|Fasching Mario]] 14:41, 26 September 2012 (CEST)
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== TPP electrode / interaction with TMPD ==
'''Observation and partial solution by customer:'''
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I emailed you last time about a problem with TMPD traveling into the TPP probe (pretty sure it was happening because the electrolyte was turning blue), and actually it became a persistent problem with several probes (at first I thought maybe there was a leak around the membrane off of one probe) (it would shift the absolute voltage values to more positive, until it the signal gets super staticky and I couldn't use the data at all). I resorted to either making sure I inverted the probe a few times to redistribute the TMPD in the electrolyte to spread it out a bit... or switching out the electrolyte (without replacing the membrane) and letting it sit overnight. And of course minimizing contact time of the probe with a solution containing TMPD.
Contribution by [[Lau G|Gigi Lau]] from [[CA Vancouver Richards JG]].
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== Experiences with the Ca2+ electrode ==
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created [[User:Fasching Mario|Fasching Mario]] 10:16, 7 November 2012 (CET)
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=== Ca2+ electrode and FCCP ===
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There are strong indications that FCCP and the [[ISE-Ca2+ Membranes]] are incompatible....[[User:Fasching Mario|Fasching Mario]] 10:19, 7 November 2012 (CET)
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Latest revision as of 09:48, 22 July 2019

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