Talk:O2k-TPP+ ISE-Module

Previous Product ID 32000-01

Other ions beside TPP+ TPMP+ , and Ca2+

Is only Ca+2 is detected with the suitable module? Or, other ions such Na+, phosphate, K+ can also be detected?

Answer: We offer membranes to detect TPP+ and Ca2+. There are ion selective membranes available e.g. from Sigma Aldrich for a wide variety of species. If cut to a circle with a diameter of 4 mm these membranes can be used in our ISE system.

Many ions may also be detected by fluorimetric based methods, see O2k-Fluorescence_LED2-Module

--Fasching Mario 09:48, 22 February 2012 (CET)

Types of connectors

Question: Are the ISE sockets a BNC cable connection?

Answer: In Series D and higher the ISE connectors consists of 2 ports: 1.) a BNC port: both poles are "live" so it can be used either for a combination electrode (like a classical pH) or for just one singe electrode that requires a separate reference electrode 2.) a 2 mm pin type connection that is used for connecting the separate reference electrodes we use for TPP and now also for pH This 2 mm pin port connects to the same electronics as the outer channel of the BNC port. See also chapter 3.2 of the ISE Manual MiPNet15.03. In the same manual you find the relevant information for older series of the oxygraph.Fasching Mario 09:22, 19 September 2012 (CEST)

Long term storage of the TPP electrode

Question: What is the procedure for long term storage of the TPP electrode?

Answer: For long time storage (several months) please disassemble the electrode, wash all parts with ethanol (sterilization), allow all parts to become completely dry, then re-assemble the dry electrode without a membrane(to have everything together and to avoid loosing small parts). Then store the electrode in a dark place like the TPP accessory box. Fasching Mario 14:41, 26 September 2012 (CEST)

TPP electrode / interaction with TMPD

Observation and partial solution by customer:

I emailed you last time about a problem with TMPD traveling into the TPP probe (pretty sure it was happening because the electrolyte was turning blue), and actually it became a persistent problem with several probes (at first I thought maybe there was a leak around the membrane off of one probe) (it would shift the absolute voltage values to more positive, until it the signal gets super staticky and I couldn't use the data at all). I resorted to either making sure I inverted the probe a few times to redistribute the TMPD in the electrolyte to spread it out a bit... or switching out the electrolyte (without replacing the membrane) and letting it sit overnight. And of course minimizing contact time of the probe with a solution containing TMPD. Contribution by Gigi Lau from CA Vancouver Richards JG.

Experiences with the Ca2+ electrode

created Fasching Mario 10:16, 7 November 2012 (CET)

Ca2+ electrode and FCCP

There are strong indications that FCCP and the ISE-Ca2+ Membranes are incompatible....Fasching Mario 10:19, 7 November 2012 (CET)

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