Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Talk:O2k-Videosupport

From Bioblast
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

Last update: 2018-04-05

Videoclip for pfi

Last update: 2018-04-05

  1. We will need some new sequences to integrate in the videoclips: we will not use liquid into the cup for taking the wet weight.

Last update: 2018-02-12

  1. 02:00 --> When BIOPS is added to the wells (plate): add the text: all wells 2 mL BIOPS. The text will stay until: 02:10.
  2. 02:18 --> We add 1 mL of BIOPS into the eppendorf tube. Show the pippete with 1 mL (instead of the 2 mL pippete). We add 1 mL of BIOPS to the Petridish as well.
  3. 03:00 --> Add text for the saponin concentration --> 5 mg/mL in BIOPS
  4. 03:37 --> Add text with the information --> 20 ยตL saponin in 2 mL BIOPS
  5. 03:42 --> Add text with the information --> BIOPS (this text will be in the first line of the plate); BIOPS+saponin (in the second line); BIOPS (in the last line)
  6. 04:41 --> Incorporate images from the binocular. Johannes: is maybe possible to do any animation (with the panel showing all the steps)?
  7. 06:49 --> Add the text: Aluminum cup on the petri dish
  8. 07:50 --> Add the text: Blotting during 40 seconds
  9. 08:13 --> Add to the video (if Johannes took it, I think he took it. Checking if the forceps are dry after taking the wet weight) --> and add the text: check the forceps
  10. 08:16 --> Add the text info?: take wet weight
  11. ~08:39-08:40 --> When we take the forceps, we can see something.
  12. ~08:56-08:59 --> Add the text: Add O2


Clips for pfi

Last update: 2018-02-12

1. Materials (00:00-01:30)
2. BIOPS and saponin (01:30-03:55)
3. Muscle fibre preparation: Mechanical Preparation (03:55-04:57)
4. Muscle fibre preparation: Chemical Permeabilization (04:57-06:33)
5. Wet Weight (06:34-08:17)
6. To the O2k (08:18-09:15)


Videoclip for Shredder

Last update: 2018-04-05

  1. We will need some new sequences to integrate in the videoclips: we will not use liquid into the cup for taking the wet weight.

Last update: 2018-02-12

  1. ~00:19 --> Add text? Calculate volume needed for your thom (here, pipette adding 5 mL, show pipette 5 mL (00:41)).
  2. 00:41 --> Not 5 mL here, show pippete with 500 ยตL.
  3. 01:38 --> Could we incorporate a video taking the wet weight from the fiber videoclip? It will be added to the Shredder videoclip (01:38, before we see the eppendorf tube with the yellow label). We can take the video-section from the fibres (To include the sequences: 06:58-07:06; 07:12-07:13 maybe here you could do a zoom showing only the Falcon tube with the MiR05-Kit; 07:17-07:21; 07:31-07:37; 07:45-08:12; include the section checking the forceps from the fibres from 08:13; 08:19-08:20. Finally show the sequence 01:39 in the Shredder clip).
  4. ~02:06 --> Text: Cut into smaller pieces
  5. ~02:21 --> Text? Distributed on the Lysis Disk
  6. ~04:02 --> 5 seconds instead of 10 seconds (in the position 2)
  7. ~07:33--> Not all the volume is take it. Does Johannes has another video?
  8. Last part of the video is in the pfi video.


Clips for Shredder

Last update: 2018-02-12

1. Materials (00:00-01:30)
2. Tissue preparation (01:30-03:02)
3. Homogenization (~03:02-04:27)
4. Retrieval (~04:27-to the end)
5. To the O2k (from 09:12 in the pfi video)


Bioblast wiki

Popular Bioblast page

O2k-Videosupport has been accessed more than
  • 10,000 times (2019-12-12)
  • 5,000 times (2018-10-29)