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MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
O2 calibration is the calibration in DatLab of the oxygen sensor. It is a prerequisite for obtaining accurate measurements of respiration. Accurate calibration of the oxygen sensor depends on (1) equilibration of the incubation medium with air oxygen partial pressure at the temperature defined by the experimenter; (2) zero oxygen calibration; (3) high stability of the POS signal tested for sufficiently long periods of time; (4) linearity of signal output with oxygen pressure in the range between oxygen saturation and zero oxygen pressure; and (5) accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration. The standard oxygen calibration procedure is described below for high-resolution respirometry with the calibration routine using instrumental calibration DL-Protocols in DatLab.
Reference: MiPNet06.03 POS-calibration-SOP, MiPNet12.20_O2k-calibration_tutorial
General Information
- why do we need calibration
- how often + link to quality control Oxygen_sensor_text: add comments about frequency there
- what do we do with the instrument
- using a protocol. With or without the protocol: we need marks R1 and R0 to proceed.
DatLab 8
- How to get R1 and R0
- calibration values are stored together with instrument identifiers and sensor numbers
- bottom left and bottom right: yellow until calibration has been confirmed
DatLab 7.4
see current content of the page
Details
- marks: what is it, how to set it, how to extract values
- events: what is it, how to set it
- special situation: one computer, several O2ks connected
Related Topics
- Quality control --> link
