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Difference between revisions of "Talk:Setting the oxygen concentration"

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(Created page with "{{Technical service}} Question: Can we set the oxygen concentration in the Oroboros-2k to desired levels? Answer: Yes. It is always possible to increase the oxygen concentratio...")
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Yes.
Yes.


It is always possible to increase the oxygen concentration using the combination of catalase in the medium and injections of H2O2, as described inย  [[MiPNet14.13 Medium-MiR06| MiPNet14.3]]. By using the [[TIP2k]] the oxygen concentration can be kept between well defined limits, either using H2O2 or (for very low oxygen concentrations) air saturated medium in the TIP syringes. We call this an "oxystat" approach and supply approbate templates for controlling the TIP2k in this matter. See also [http://www.oroboros.at/index.php?id=o2k-tip2k-manual MiPNet12.10]. ย 
It is always possible to increase the oxygen concentration using the combination of catalase in the medium and injections of H2O2, as described inย  [[MiPNet14.13 Medium-MiR06| MiPNet14.3]]. By using the [[TIP2k]] the oxygen concentration can be maintained between well defined limits, either using H2O2 or (for very low oxygen concentrations) air saturated medium in the TIP syringes. We call this an "oxystat" approach and supply appropriate templates for controlling the TIP2k in. See also [http://www.oroboros.at/index.php?id=o2k-tip2k-manual MiPNet12.10]. ย 


This leaves the question how low oxygen levels are reached in the first place: In a biological experiment this may be done by inserting a N2 bubble into the chamber, waiting until the desired level is reached and then destroying the air bubble by closing the stoppers. However, most often the desired starting value is simply reached by waiting until the sample has consumed the required amount of oxygen and then starting the "oxystat".
This leaves the question how low oxygen levels are reached in the first place: In a biological experiment this may be done by inserting a N2 bubble into the chamber, waiting until the desired level of oxygen is reached and then destroying the air bubble by closing the stoppers. However, most often the desired starting value is simply reached by waiting until the sample has consumed the required amount of oxygen and then starting the "oxystat".
For calibration and instrumental oxygen background purposes we used Dithionite to reduce oxygen levels, however this is not recommended in the presence of biological samples.
For calibration and instrumental oxygen background purposes we use Dithionite to reduce oxygen levels, however this is not recommended in the presence of biological samples.


{{#set:Technical service=Chamber|Technical service=O2 signal}}
{{#set:Technical service=Chamber|Technical service=O2 signal}}

Revision as of 17:28, 23 May 2015

Template:Technical service Question: Can we set the oxygen concentration in the Oroboros-2k to desired levels?

Answer: Yes.

It is always possible to increase the oxygen concentration using the combination of catalase in the medium and injections of H2O2, as described in MiPNet14.3. By using the TIP2k the oxygen concentration can be maintained between well defined limits, either using H2O2 or (for very low oxygen concentrations) air saturated medium in the TIP syringes. We call this an "oxystat" approach and supply appropriate templates for controlling the TIP2k in. See also MiPNet12.10.

This leaves the question how low oxygen levels are reached in the first place: In a biological experiment this may be done by inserting a N2 bubble into the chamber, waiting until the desired level of oxygen is reached and then destroying the air bubble by closing the stoppers. However, most often the desired starting value is simply reached by waiting until the sample has consumed the required amount of oxygen and then starting the "oxystat". For calibration and instrumental oxygen background purposes we use Dithionite to reduce oxygen levels, however this is not recommended in the presence of biological samples.