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Difference between revisions of "Template:BioblastAlert2021"

From Bioblast
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=== Bioblast alert 2021(07) - Diabetes: 2021-04-15 ===
:{|
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Diabetes, infarct size, mitochondrial respiration and succinate dehydrogenase inhibition - how do they relate?''' <br>
: from the O2k-Network »[[DK Aarhus Boetker HE]]« <br>
* Tonnesen PT, Hjortbak MV, Lassen TR, Seefeldt JM, Bøtker HE, Jespersen NR (2021) Myocardial salvage by succinate dehydrogenase inhibition in ischemia-reperfusion injury depends on diabetes stage in rats. Mol Cell Biochem [Epub ahead of print]. - [[Tonnesen 2021 Mol Cell Biochem |»Bioblast link«]]
|}
:{|
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Gastric bypass surgery improves diabetic nephropathy in type 1 diabetic rats''' <br>
: from the O2k-Network »[[CN Chongqing Zhu Z]]« <br>
* Wei X, Lu Z, Li L, Zhang H, Sun F, Ma H, Wang L, Hu Y, Yan Z, Zheng H, Yang G, Liu D, Tepel M, Gao P, Zhu Z (2020) Reducing NADPH synthesis counteracts diabetic nephropathy through restoration of AMPK activity in type 1 diabetic rats. Cell Rep 32:108207.  - [[Wei 2020 Cell Rep|»Bioblast link«]]
|}
:{|
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Skeletal muscle mitochondrial bioenergetics in men and women with type 1 diabetes''' <br>
: from the O2k-Network »[[CA Toronto Perry CG]]« <br>
* Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - [[Monaco 2020 Am J Physiol Endocrinol Metab|»Bioblast link«]]
|}
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=== Bioblast alert 2021(06) - Fatty acid oxidation (FAO): 2021-03-19 ===
=== Bioblast alert 2021(06) - Fatty acid oxidation (FAO): 2021-03-19 ===
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Revision as of 09:24, 15 April 2021



Bioblast alert 2021(07) - Diabetes: 2021-04-15

O2k-Publications in the MiPMap Diabetes, infarct size, mitochondrial respiration and succinate dehydrogenase inhibition - how do they relate?
from the O2k-Network »DK Aarhus Boetker HE« 
  • Tonnesen PT, Hjortbak MV, Lassen TR, Seefeldt JM, Bøtker HE, Jespersen NR (2021) Myocardial salvage by succinate dehydrogenase inhibition in ischemia-reperfusion injury depends on diabetes stage in rats. Mol Cell Biochem [Epub ahead of print]. - »Bioblast link«
O2k-Publications in the MiPMap Gastric bypass surgery improves diabetic nephropathy in type 1 diabetic rats
from the O2k-Network »CN Chongqing Zhu Z« 
  • Wei X, Lu Z, Li L, Zhang H, Sun F, Ma H, Wang L, Hu Y, Yan Z, Zheng H, Yang G, Liu D, Tepel M, Gao P, Zhu Z (2020) Reducing NADPH synthesis counteracts diabetic nephropathy through restoration of AMPK activity in type 1 diabetic rats. Cell Rep 32:108207. - »Bioblast link«
O2k-Publications in the MiPMap Skeletal muscle mitochondrial bioenergetics in men and women with type 1 diabetes
from the O2k-Network »CA Toronto Perry CG« 
  • Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - »Bioblast link«

Bioblast alert 2021(06) - Fatty acid oxidation (FAO): 2021-03-19

O2k-Publications in the MiPMap Approved FAO inhibitor Trimetazidine restricts tumor growth in oxidative lung carcinomas
from the O2k-Network »FR Bordeaux Rossignol R« 
  • Amoedo ND, Sarlak S, Obre E, Esteves P, Bégueret H, Kieffer Y, Rousseau B, Dupis A, Izotte J, Bellance N, Dard L, Redonnet-Vernhet I, Punzi G, Rodrigues MF, Dumon E, Mafhouf W, Guyonnet-Dupérat V, Gales L, Palama T, Bellvert F, Dugot-Senan N, Claverol S, Baste JM, Lacombe D, Rezvani HR, Pierri CL, Mechta-Grigoriou F, Thumerel M, Rossignol R (2021) Targeting the mitochondrial trifunctional protein restrains tumor growth in oxidative lung carcinomas. J Clin Invest 131:e133081. - »Bioblast link«
MitoPedia
CI inhibitors like rotenone inhibit FAO-linked respiration

Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD+ blocks FAO.
- »Fatty acid oxidation pathway control state« 

Gnaiger 2020 BEC MitoPathways Measuring FAO-linked respiration requires a low concentration of malate

In the absence of malate, accumulation of acetyl-CoA inhibits FAO.
- »Gnaiger 2020 BEC MitoPathways - Chapter 5« 

MitoPedia: SUIT Quantifying FAO capacity (F-pathway) - avoid overestimation

Malate anaplerotic pathways are active in various tissues and cultured cells, which can lead to an overestimation of the F-pathway capacity. .
- Evaluate the malate anaplerotic pathway: »SUIT-027« 

- Analyze F-pathway avoiding overestimation: »SUIT-002« and »SUIT-025« 


Bioblast alert 2021(05) - Bioenerg Commun & MitoFit Preprints: 2021-03-04

MitoFit pdf Mitochondrial physiology in Parkinson’s disease (PD)

A COST Action MitoEAGLE contribution:

  • Krako Jakovljevic N, Ebanks B, Chakrabarti L, Markovic I, Moisoi N (2021) Mitochondrial homeostasis in cellular models of Parkinson’s Disease. MitoFit Preprints 2021.4 doi:10.26124/mitofit:2021-0004
Gnaiger 2020 BEC MitoPathways Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?

A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3« 

Bioenergetics Communications Submit manuscripts simultaneously to MitoFit Preprints and Bioenergetics Communications via the BEC website

A seamless process for taking MitoFit preprints to living BEC communications with open and published peer review.

- »Bioenergetics Communications«


Bioblast alert 2021(04) - MitoFit Preprints: 2021-02-19

MitoFit pdf Exploring the Q-junction in real-time

Simultaneous measurement of coenzyme Q redox state and respiration in isolated mitochondria:

  • Komlodi T, Cardoso LHD, Doerrier C, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2. doi:10.26124/mitofit:2021-0002
MitoFit pdf Diagnosis of mitochondrial disease in frozen samples

Measurement of respiratory function in cryopreserved muscle biopsies:

  • Zuccolotto-dos-Reis FH, Andriao-Escarso SH, Araujo JS, Espreafico EM, Alberici LC, Sobreira CFR (2021) Acetyl CoA driven respiration in frozen muscle contributes to the diagnosis of mitochondrial disease. MitoFit Preprints 2021.3. doi:10.26124/mitofit:2021-0003

Bioblast alert 2021(03) - ATP production P»: 2021-01-29

MitoFit pdf SUIT protocols with Magnesium Green™ (MgG) can be used to study respiration and ATP production P»

MgG dye does not affect respiration of cardiac isolated mitochondria.

  • Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001
O2k-brief repository Measuring ATP-ADP exchange using the fluorophore Magnesium Green
from the O2k-Network »HU Budapest Chinopoulos C« 
  • Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«
MitoPedia
Magnesium Green and P» – which sample preparations work?

Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG« 

MitoPedia: SUIT Did you know that you can measure the respiratory capacity available for P» in living cells?

SUIT protocols from the SUIT-003 family guide you through the titrations to analyze R-L net ROUTINE capacity.
- »SUIT-003 O2 ce D009« and »SUIT-003 O2 ce-pce D020« 


Bioblast alert 2021(02) - Permeabilized fibers: 2021-01-21

Gnaiger 2020 BEC MitoPathways A quote from MitoPathways Chapter 3. Normalization:

Respiratory OXPHOS capacity P in human skeletal muscle ranges from 60 to 160 pmol∙s­-1∙mg­-1 based on wet muscle mass of permeabilized fibers. By comparison, mass-specific electron transfer capacity E is approximately 25 pmol∙s-1∙mg-1 in platelets and senescent fibroblasts and 40 to 80 pmol∙s-1∙mg-1 in PBMC, HEK, CEM, HUVEC, and young fibroblasts. ― Are these values comparable? - »Gnaiger 2020 BEC MitoPathways«.

O2k-Publications in the MiPMap Statistical evaluation on replica in high-resolution respirometry with permeabilized skeletal muscle fibers
from the O2k-Network »AU Melbourne Bishop DJ« 
  • Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. - »Bioblast link«
O2k-Publications in the MiPMap Effect of temperature on mitochondrial respiratory capacity of cardiac permeabilized fibers
from the O2k-Network »AT Innsbruck Gnaiger E« , »CA Rimouski Blier PU« and »CA Edmonton Lemieux H«
  • Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. »Bioblast link«
MitoPedia
What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?

For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.
- »Optimum ADP concentration in pfi« 


Bioblast alert 2021(01) - MitoPathways: the new BEC edition of the Blue Book: 2021-01-05

Gnaiger 2020 BEC MitoPathways The 5th edition of MitoPathways features new chapters on additivity of mitochondrial pathway capacities and protonmotive pressure – one step beyond ∆Ψ and the protonmotive force.