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Difference between revisions of "Template:Current week ecosystem agenda"

From Bioblast
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| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]  
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]  
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br>
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br>
:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable)<br>
:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable).<br>
- » [[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«
- »[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«
|}
|}



Revision as of 09:49, 30 June 2020

 2020 Week 27 
  • Jun-29 Mo
O2k-Open SupportWarning: loss of data Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?
An 'O2 slope neg.' within ± 2 pmol∙s−1∙mL−1 is optimal (± 4 pmol∙s−1∙mL−1 is acceptable).

- » Air and zero calibration«


  • Jun-30 Tu
O2k-Publications in the MiPMap The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice
from the O2k-Network »LV Riga Makrecka-Kuka M«
  • Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. »Bioblast link«

  • Jul-01 We

  • Jul-02 Th
MitoPedia
Did you know that if ET capacity E.jpg is lower than OXPHOS capacity P.jpg (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:

- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity.

Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link« 


  • Jul-03 Fr
O2k-brief repository Mitochondrial H2O2 flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration
from the O2k-Network »US CO Fort Collins Chicco AJ« 
  • Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. »Bioblast link«