Difference between revisions of "Template:Current week ecosystem agenda"

Revision as of 08:19, 12 October 2020

2020 Week 42

Oct-12 Mo Targeting Metabesity 2020 Virtual Event Oct 12-15

If using the Amplex UltraRed assay, when should the background H₂O₂ calibration be performed?

Background H₂O₂ calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H₂O₂ is prepared, or if a different respiration medium is used. As the H₂O₂ stock solution needs to be freshly prepared, it is recommended to perform a background H₂O₂ calibration before each experiment and at a minimum once per experimental day.
- »How to find the background H₂O₂ calibration protocol in DatLab « select “AmR calibration” for the background H₂O₂ calibration protocol
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Oct-13 Tu

»FAT4BRAIN School IOC147 Virtual Event«

Oct-14 We

Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction?

from the O2k-Network »US VA Richmond Lesnefsky EJ«

Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. »Bioblast link«

Oct-15 Th

How can you reduce the amount of biological sample needed for high-resolution respirometry?

The O2k-sV-Module provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.
- »O2k-sV-Module«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Oct-16 Fr

Oct-17 Sa

Oct-18 Su

»ISCaM 2020 Virtual Event«

@@ Line 1: / Line 1: @@
-Week 41
+Week 42
-* '''Oct-05 Mo''' [[File:O2kHandOver 20201002.jpg|right|200px]]
+* '''Oct-12 Mo''' [http://www.metabesity2020.com/ Targeting Metabesity 2020 Virtual Event] Oct 12-15
 :{|
-| width="100" align="center" |[[Image:FFG-logo.png|70px|FFG]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Dr. Markus Keller and his team have now received the O2ks for their measurements in the FFG-funded TissueModels project!'''<br>
+|'''If using the [[Amplex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed?
-The project aims to establish physiological models for specific tissue types. Oroboros Instruments is a consortium partner, providing infrastructure and expertise.
+Background H<sub>2</sub>O<sub>2</sub> calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H<sub>2</sub>O<sub>2</sub> is prepared, or if a different respiration medium is used. As the H<sub>2</sub>O<sub>2</sub> stock solution needs to be freshly prepared, it is recommended to perform a background H<sub>2</sub>O<sub>2</sub> calibration before each experiment and at a minimum once per experimental day. <br>
+- »[[Instrumental:_Browse_DL-Protocols_and_templates|How to find the background H<sub>2</sub>O<sub>2</sub> calibration protocol in DatLab]] « select  “AmR calibration”  for the background H<sub>2</sub>O<sub>2</sub> calibration protocol <br>
-- Read more »'''[[TissueModels]]'''«
+- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
 |}
+----
+* '''Oct-13 Tu'''
+:::: [[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] »<big>'''[[FAT4BRAIN School IOC147 Virtual Event]]'''</big>«
 ----
-* '''Oct-06 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]]
+* '''Oct-14 We'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Fasting promotes functional changes in liver mitochondria: Is it time to reconsider using fasted animals for liver mitochondria isolation?'''<br>
+|'''Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction? '''<br>
-:from the O2k-Network »[[BR Sao Paulo Kowaltowski AJ]]«
+:from the O2k-Network »[[US VA Richmond Lesnefsky EJ]]« <br>
-*Menezes-Filho SL, Amigo I, Luévano-Martínez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35. [[Menezes-Filho 2018 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
+*Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. [[Mohsin 2020 Arch Biochem Biophys|»Bioblast link«]]
 |}
 ----
-* '''Oct-07 We'''
+* '''Oct-15 Th'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Background H<sub>2</sub>O<sub>2</sub> calibration: what is it and why perform it when using Amplex UltraRed assay?'''<br>
+|''' How can you reduce the amount of biological sample needed for high-resolution respirometry?  '''<br>
-Background H<sub>2</sub>O<sub>2</sub> calibration refers to a set of H<sub>2</sub>O<sub>2</sub> titrations conducted after addition of respiration medium, DTPA, HRP, SOD and Amplex UltraRed to the O2k-chamber but before sample addition. This step is required 1) for the calculation of the theoretical chemical background fluorescence in our Excel analysis template, and 2) for the calculation of the sensitivity of the AmR assay, which serves as a quality control of the assay. <br>
+The O2k-sV-Module  provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.   <br>
-- »'''[[Amplex_UltraRed#Calibration_with_H2O2|Background H<sub>2</sub>O<sub>2</sub> calibration]]'''«  <br>
+- »'''[[O2k-sV-Module]]'''«  <br>
 - communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
 |}
+----
+* '''Oct-16 Fr'''
+----
+* '''Oct-17 Sa'''
 ----
-* '''Oct-08 Th'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Maternal childhood maltreatment is associated with increased mitochondrial bioenergetic function in mothers, but not newborns'''<br>
-:from the O2k-Network »[[DE Ulm Gumpp A]]«
-*Gumpp AM, Boeck C, Behnke A, Bach AM, Ramo-Fernández L, Welz T, Gündel H, Kolassa IT, Karabatsiakis A (2020) Childhood maltreatment is associated with changes in mitochondrial bioenergetics in maternal, but not in neonatal immune cells. Proc Natl Acad Sci U S A 117:24778-84.  [[Gumpp 2020 Proc Natl Acad Sci U S A|»Bioblast link«]]
-|}
-----
+* '''Oct-18 Su'''
-* '''Oct-09 Fr'''
+:::: »<big>'''[[ISCaM 2020 Virtual Event]]'''</big>«
-:::: »<big>'''[[Long Night of Research 2020 Innsbruck AT]]'''</big>«