Difference between revisions of "Template:Current week ecosystem agenda"

Revision as of 08:25, 19 October 2020

2020 Week 43

Oct-19 Mo

Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?

Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.
- You can read more about it in the following publication: »Krumschnabel 2014 Methods Enzymol«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«

Oct-20 Tu - ÖGMBT Life Science Tuesdays

Oct-21 We

Blood cell respirometry might be an attractive alternative to skeletal- and cardiac muscle-based assessment of mitochondrial function in human studies

from the O2k-Network »US NC Winston-Salem Molina AJA«

Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77.»Bioblast link«

Oct-22 Th

LEAK respiration relates to intrinsic uncoupling.

Non-phosphorylating LEAK respiration is measured in the absence of externally added uncouplers (i) in the absence of adenylates, (ii) after depletion of ADP, or (iii) after inhibition of ATP synthase or adenine nucleotide translocase.
- »BEC2020.1: Mitochondrial physiology« - Table 2. Terms on respiratory coupling and uncoupling
- communicated by »Komlodi Timea« and »Cecatto Cristiane« and »Gnaiger Erich«

Oct-23 Fr

Simultaneous measurement of O₂ flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system

from the O2k-Network »AT Innsbruck Oroboros« and »AT Innsbruck Gnaiger E«

Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Bioblast link«

@@ Line 1: / Line 1: @@
-Week 42
+Week 43
-* '''Oct-12 Mo''' [http://www.metabesity2020.com/ Targeting Metabesity 2020 Virtual Event] Oct 12-15
+* '''Oct-19 Mo'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''If using the [[Amplex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed?
+|'''Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?'''<br>
-Background H<sub>2</sub>O<sub>2</sub> calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H<sub>2</sub>O<sub>2</sub> is prepared, or if a different respiration medium is used. As the H<sub>2</sub>O<sub>2</sub> stock solution needs to be freshly prepared, it is recommended to perform a background H<sub>2</sub>O<sub>2</sub> calibration before each experiment and at a minimum once per experimental day. <br>
+Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.<br>
-- »[[Instrumental:_Browse_DL-Protocols_and_templates|How to find the background H<sub>2</sub>O<sub>2</sub> calibration protocol in DatLab]] « select  “AmR calibration”  for the background H<sub>2</sub>O<sub>2</sub> calibration protocol <br>
+-  You can read more about it in the following publication: »[[Krumschnabel 2014 Methods Enzymol]]« <br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
+- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]«
 |}
 ----
-* '''Oct-13 Tu'''
+* '''Oct-20 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]]
-:::: [[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] »<big>'''[[FAT4BRAIN School IOC147 Virtual Event]]'''</big>«
 ----
-* '''Oct-14 We'''
+* '''Oct-21 We'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction? '''<br>
+|'''Blood cell respirometry might be an attractive alternative to skeletal- and cardiac muscle-based assessment of mitochondrial function in human studies '''<br>
-:from the O2k-Network »[[US VA Richmond Lesnefsky EJ]]« <br>
+:from the O2k-Network »[[US NC Winston-Salem Molina AJA]]«
-*Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. [[Mohsin 2020 Arch Biochem Biophys|»Bioblast link«]]
+*Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77.[[Tyrrell 2016 Redox Biol|»Bioblast link«]]
 |}
 ----
-* '''Oct-15 Th'''
+* '''Oct-22 Th'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' How can you reduce the amount of biological sample needed for high-resolution respirometry?  '''<br>
+| width="110" align="center" | [[File:BEC-logo.png|left|100px|link=Bioenergetics Communications|Bioenergetics Communications]]
-The O2k-sV-Module  provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.   <br>
+|''' LEAK respiration relates to intrinsic uncoupling.   '''<br>
-- »'''[[O2k-sV-Module]]'''«  <br>
+Non-phosphorylating LEAK respiration is measured in the absence of externally added uncouplers (i) in the absence of adenylates, (ii) after depletion of ADP, or (iii) after inhibition of ATP synthase or adenine nucleotide translocase.  <br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
+- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC2020.1: Mitochondrial physiology]'''« - Table 2. Terms on respiratory coupling and uncoupling  <br>
+- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]« and »[[Gnaiger Erich]]«
 |}
 ----
-* '''Oct-16 Fr'''
+* '''Oct-23 Fr'''
 :{|
-| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
+| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
-| '''Which SUIT protocol can you use to evaluate mitochondrial respiration of [[PBMC]] and [[Platelet|platelets]]?'''<br>
+|'''Simultaneous measurement of O<sub>2</sub> flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system''' <br>
-The following SUIT protocols will guide you through the titration experiments for these cell models <br>
+: from the O2k-Network »[[AT Innsbruck Oroboros]]« and »[[AT Innsbruck Gnaiger E]]«<br>
-- »'''[[SUIT-001 O2 ce-pce D004]]'''« and »'''[[SUIT-002 O2 ce-pce D007a]]'''« <br>
+* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.   [[Krumschnabel 2014 Methods Enzymol|»Bioblast link«]]
-- communicated by »[[Cecatto Cristiane]]« and »[[Garcia-Souza Luiz F]]«
 |}
-----
-* '''Oct-17 Sa'''
-----
-* '''Oct-18 Su'''
-:::: »<big>'''[[ISCaM 2020 Virtual Event]]'''</big>«