Template:Current week ecosystem agenda

2020 Week 42

Oct-12 Mo Targeting Metabesity 2020 Virtual Event Oct 12-15

If using the Amplex UltraRed assay, when should the background H₂O₂ calibration be performed?

Background H₂O₂ calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H₂O₂ is prepared, or if a different respiration medium is used. As the H₂O₂ stock solution needs to be freshly prepared, it is recommended to perform a background H₂O₂ calibration before each experiment and at a minimum once per experimental day.
- »How to find the background H₂O₂ calibration protocol in DatLab « select “AmR calibration” for the background H₂O₂ calibration protocol
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Oct-13 Tu

»FAT4BRAIN School IOC147 Virtual Event«

Oct-14 We

Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction?

from the O2k-Network »US VA Richmond Lesnefsky EJ«

Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. »Bioblast link«

Oct-15 Th

How can you reduce the amount of biological sample needed for high-resolution respirometry?

The O2k-sV-Module provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.
- »O2k-sV-Module«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Oct-16 Fr

Which SUIT protocol can you use to evaluate mitochondrial respiration of PBMC and platelets?

The following SUIT protocols will guide you through the titration experiments for these cell models
- »SUIT-001 O2 ce-pce D004« and »SUIT-002 O2 ce-pce D007a«
- communicated by »Cecatto Cristiane« and »Garcia-Souza Luiz F«

Oct-17 Sa

Oct-18 Su

»ISCaM 2020 Virtual Event«