Difference between revisions of "Template:Next oroboros ecosystem agenda"

Revision as of 16:20, 3 September 2020

2020 Week 37

Sep-07 Mo

Why it is necessary to perform multiple H₂O₂ calibrations when using the Amplex UltraRed (AmR) assay?

The sensitivity to H₂O₂ of the Amplex UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of: 1) experimental time, 2) changes in the optical properties of the respiration medium due to sample/chemical titration, 3) the radical scavenging capacity of the sample, and 4) the concentration of accumulating resorufin (UltroxRed in the case of Amplex UltraRed). Therefore, it is recommended to perform H₂O₂ calibration before sample addition, after sample addition and multiple times during the experiment.
- »Komlodi 2018 Methods Mol Biol«

The DLPs for AmR SUIT protocols already come with multiple steps of H₂O₂ calibrations implemented.
- » SUITbrowser«
- communicated by »Komlodi Timea« and »Cardoso Luiza«

Sep-08 Tu

Sep-09 We

Sep-10 Th

What is the limitation of State 3 respiration compared to OXPHOS capacity?

OXPHOS capacity is defined as the maximal respiratory capacity in the OXPHOS state in the presence of kinetically-saturating concentration of ADP, P_i, fuel substrates and O₂. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of OXPHOS capacity and overestimation of excess E-P capacity due to kinetic limitations of the phosphorylation system.
- »BEC: Mitochondrial physiology« - Table 3 and several figures.
- communicated by » Cecatto Cristiane « and » Komlodi Timea «

Sep-11 Fr

2020 Week 38

Sep-14 Mo

Why are permeabilized muscle fibers (pfi) a poor sample type for studying reactive oxygen species (ROS) production?

In experiments with pfi, high oxygen concentrations are needed to avoid oxygen limitation . However, the high oxygen pressures used may artificially increase ROS, including H₂O₂ production, making pfi a less optimal model for ROS production studies.
- »Oxygen dependence of ROS production in pfi« - communicated by »Komlodi Timea« and »Cardoso Luiza«

Sep-15 Tu

Sep-16 We

Sep-17 Th

Did you know that you can measure the respiratory activity available for phosphorylation of ADP to ATP in living cells?

SUIT protocols from the SUIT-003 family will guide you through the titrations needed to analyse free ROUTINE activity.
- »SUIT-003 O2 ce D009« and »SUIT-003 O2 ce-pce D020«
- communicated by »Cardoso Luiza« and »Komlodi Timea«

Sep-18 Fr

Sep-19 Sa

Sep-20 So

2020 Week 39

Sep-21 Mo

12th ÖGMBT Annual Meeting 2020 Graz AT Sep 21-23

Did you know the reverse electron flow from Complex II to Complex I can be investigated in mitochondrial preparations by measuring hydrogen peroxide production using Amplex UltraRed assay?

The reverse electron flow, also called reverse electron transfer (RET), happens when electrons are transferred from Complex II to the Q-junction and then back to Complex I at high mt-membrane potential. This phenomenon results in high hydrogen peroxide production in some mitochondrial preparations. The SUIT-026 protocol was designed to investigate this effect.
- »SUIT-026«
- communicated by »Komlodi Timea« and »Cardoso Luiza«

Sep-22 Tu - 12th ÖGMBT Annual Meeting 2020 Graz AT

Sep-23 We - 12th ÖGMBT Annual Meeting 2020 Graz AT

Sep-24 Th

18:00 - Houska Awards 2020 Vienna - Houskapreis der B&C Privatstiftung - Verleihung des Houskapreises 2020 in Wien

Sep-25 Fr

2020 Week 40

Sep-28 Mo

Sep-29 Tu

Sep-30 We

Early registration deadline - »ESSR 2020 Innsbruck AT«

Oct-01 Th

Oct-02 Fr

Oct-03 Sa

2020 Week 41

Oct-05 Mo

Oct-06 Tu

Oct-07 We

Pre-Conference O2k-Workshop »MiPNet25.11 IOC148 Riga LV «

»6th EU-Cardioprotection Meeting 2020 Riga LV« (Oct 7-9)

Oct-08 Th - 6th EU-Cardioprotection Meeting 2020 Riga LV

Oct-09 Fr - 6th EU-Cardioprotection Meeting 2020 Riga LV

»Long Night of Research 2020 Innsbruck AT«

2020 Week 42

Oct-12 Mo

»FAT4BRAIN School IOC147 Riga LV« (Oct 7-9)

Oct-13 Tu

FAT4BRAIN School IOC147 Riga LV

Oct-14 We

Oct-15 Th

Oct-16 Fr

Oct-18 Su

»ISCaM 2020 London UK«

2020 Week 43

Oct-19 Mo - ISCaM 2020 London UK

Oct-20 Tu - ISCaM 2020 London UK

Oct-21 We

Oct-22 Th

Oct-23 Fr

2020 Week 44

Oct-26 Mo

Oct-27 Tu

Oct-28 We

Oct-29 Th

Oct-30 Fr

2020 Week 45

Nov-02 Mo

Nov-03 Tu

Nov-04 We

MitoEAGLE Summit Obergurgl 2020

Nov-05 Th - MitoEAGLE Summit Obergurgl 2020

Nov-06 Fr - MitoEAGLE Summit Obergurgl 2020
Nov-07 Sa - MitoEAGLE Summit Obergurgl 2020

2020 Week 46

Nov-09 Mo

MiPschool Obergurgl 2020 including TRANSMIT School 2020

Nov-10 Tu - MiPschool Obergurgl 2020 including TRANSMIT School 2020

Nov-11 We - MiPschool Obergurgl 2020 including TRANSMIT School 2020

Nov-12 Th

Nov-13 Fr

2020 Week 47

Nov-16 Mo

Nov-17 Tu

Nov-18 We

ASMRM 2020 Singapore SG

Nov-19 Th

ASMRM 2020 Singapore SG

Nov-20 Fr

ASMRM 2020 Singapore SG

2020 Week 48

Nov-23 Mo

Nov-24 Tu

Nov-25 We

» 2020Spring PaduaMuscleDays Padova IT /

IOC Padua IT - separate registration

Nov-26 Th

2020Spring PaduaMuscleDays Padova IT

Nov-27 Fr

2020Spring PaduaMuscleDays Padova IT

Nov-28 Sa

2020Spring PaduaMuscleDays Padova IT

2020 Week 49

Nov-30 Mo

Dec-01 Tu

AlgaEurope 2020 Rome IT

Dec-02 We

Dec-03 Th

Dec-04 Fr

Dec-05 Sa

Dec-06 So

Registration deadline - »ESSR 2020 Innsbruck AT«

2020 Week 50

Dec-07 Mo

Dec-08 Tu

Dec-09 We

ESSR 2020 Innsbruck AT (Dec 9-11)

Dec-10 Th

Dec-11 Fr

2020 Week 51

Dec-14 Mo

Dec-15 Tu

Dec-16 We

Dec-17 Th

Dec-18 Fr

2020 Week 52

Dec-21 Mo

Dec-22 Tu

Dec-23 We

Dec-24 Th

Dec-25 Fr

2020 Week 53

Dec-28 Mo

Dec-29 Tu

Dec-30 We

Dec-31 Th

Jan-01 Fr

@@ Line 41: / Line 41: @@
 In experiments with pfi, high oxygen concentrations are needed to avoid oxygen limitation . However, the high oxygen pressures used may artificially increase ROS, including H<sub>2</sub>O<sub>2</sub> production, making pfi a less optimal model for ROS production studies. <br>
 - »'''[[Permeabilized_muscle_fibers#Oxygen_dependence_of_ROS_production_-_are_permeabilized_fibers_a_valid_model.3F|Oxygen dependence of ROS production in pfi]]'''«
+- communicated by »[[Komlodi Timea]]« and »[[Cardoso Luiza]]«
 |}
-- communicated by »[[Komlodi Timea]]« and »[[Cardoso Luiza]]«
 ----
 * '''Sep-15 Tu'''