Difference between revisions of "Template:Next oroboros ecosystem agenda"

Revision as of 08:20, 12 October 2020

2020 Week 43

Oct-19 Mo

Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?

Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.
- You can read more about it in the following publication: »Krumschnabel 2014 Methods Enzymol«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«

Oct-20 Tu - ÖGMBT Life Science Tuesdays

Oct-21 We

Oct-22 Th

LEAK respiration relates to intrinsic uncoupling.

Non-phosphorylating LEAK respiration is measured in the absence of externally added uncouplers (i) in the absence of adenylates, (ii) after depletion of ADP, or (iii) after inhibition of ATP synthase or adenine nucleotide translocase.
- »BEC2020.1: Mitochondrial physiology« - Table 2. Terms on respiratory coupling and uncoupling
- communicated by »Komlodi Timea« and »Cecatto Cristiane« and »Gnaiger Erich«

Oct-23 Fr

Simultaneous measurement of O₂ flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system

from the O2k-Network »AT Innsbruck Oroboros« and »AT Innsbruck Gnaiger E«

Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Bioblast link«

2020 Week 44

Oct-26 Mo

Oct-27 Tu

Oct-28 We

Oct-29 Th

Oct-30 Fr

2020 Week 45

Nov-02 Mo

Nov-03 Tu - ÖGMBT Life Science Tuesdays

Nov-04 We

MitoEAGLE Summit Obergurgl 2020

Nov-05 Th - MitoEAGLE Summit Obergurgl 2020

Nov-06 Fr - MitoEAGLE Summit Obergurgl 2020

Registration deadline for: Integrative Physiology of Exercise 2020 Virtual Event

Nov-07 Sa - MitoEAGLE Summit Obergurgl 2020

2020 Week 46

Nov-09 Mo

MiPschool Obergurgl 2020

Integrative Physiology of Exercise 2020 Virtual Event Nov 9- 13

Nov-10 Tu - MiPschool Obergurgl 2020, Integrative Physiology of Exercise 2020 Virtual Event

Nov-11 We - MiPschool Obergurgl 2020, Integrative Physiology of Exercise 2020 Virtual Event

Nov-12 Th - Integrative Physiology of Exercise 2020 Virtual Event

Nov-13 Fr - Integrative Physiology of Exercise 2020 Virtual Event

2020 Week 47

Nov-16 Mo

Nov-17 Tu - ÖGMBT Life Science Tuesdays

Nov-18 We

ASMRM 2020 Singapore SG

Nov-19 Th

ASMRM 2020 Singapore SG

Nov-20 Fr

ASMRM 2020 Singapore SG

2020 Week 48

Nov-23 Mo

Nov-24 Tu

Nov-25 We

» 2020Spring PaduaMuscleDays Padova IT /

IOC Padua IT - separate registration

Nov-26 Th

2020Spring PaduaMuscleDays Padova IT

Nov-27 Fr

2020Spring PaduaMuscleDays Padova IT

Nov-28 Sa

2020Spring PaduaMuscleDays Padova IT

2020 Week 49

Nov-30 Mo

Dec-01 Tu - ÖGMBT Life Science Tuesdays

AlgaEurope 2020 Rome IT

Dec-02 We

Dec-03 Th

Dec-04 Fr

Dec-05 Sa

Dec-06 So

Registration deadline - »ESSR 2020 Innsbruck AT«

2020 Week 50

Dec-07 Mo

Dec-08 Tu

Dec-09 We

ESSR 2020 Innsbruck AT (Dec 9-11)

Dec-10 Th

Dec-11 Fr

2020 Week 51

Dec-14 Mo

Dec-15 Tu - ÖGMBT Life Science Tuesdays

Dec-16 We

Dec-17 Th

Dec-18 Fr

2020 Week 52

Dec-21 Mo

Dec-22 Tu

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2020 Week 53

Dec-28 Mo

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-Week 42
-* '''Oct-12 Mo''' [http://www.metabesity2020.com/ Targeting Metabesity 2020 Virtual Event] Oct 12-15
-:{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''If using the [[Amplex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed?
-Background H<sub>2</sub>O<sub>2</sub> calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H<sub>2</sub>O<sub>2</sub> is prepared, or if a different respiration medium is used. As the H<sub>2</sub>O<sub>2</sub> stock solution needs to be freshly prepared, it is recommended to perform a background H<sub>2</sub>O<sub>2</sub> calibration before each experiment and at a minimum once per experimental day. <br>
-- »[[Instrumental:_Browse_DL-Protocols_and_templates|How to find the background H<sub>2</sub>O<sub>2</sub> calibration protocol in DatLab]] « select  “AmR calibration”  for the background H<sub>2</sub>O<sub>2</sub> calibration protocol <br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
-|}
-----
-* '''Oct-13 Tu'''
-:::: [[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] »<big>'''[[FAT4BRAIN School IOC147 Virtual Event]]'''</big>«
-----
-* '''Oct-14 We'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction? '''<br>
-:from the O2k-Network »[[US VA Richmond Lesnefsky EJ]]« <br>
-*Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. [[Mohsin 2020 Arch Biochem Biophys|»Bioblast link«]]
-|}
-----
-* '''Oct-15 Th'''
-:{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' How can you reduce the amount of biological sample needed for high-resolution respirometry?  '''<br>
-The O2k-sV-Module  provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.   <br>
-- »'''[[O2k-sV-Module]]'''«  <br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
-|}
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-* '''Oct-16 Fr'''
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-* '''Oct-18 Su'''
-:::: »<big>'''[[ISCaM 2020 Virtual Event]]'''</big>«
 Week 43