Difference between revisions of "Template:O2k-OpenSupportAlert2020"

From Bioblast
Jump to: navigation, search
Line 32: Line 32:
 
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]  
 
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]  
 
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br>
 
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br>
: An 'O2 slope neg.' within the range of ± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable<br>
+
: An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable)<br>
 
- » [[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«
 
- » [[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«
 
|}
 
|}

Revision as of 10:46, 30 June 2020

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

Jun 2020

  • Jun-01 Mo
O2k-Open SupportWarning: loss of data Did you know that instabilities of the slope of the oxygen signal (O2 slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O2 background test?

- »Unstable 'O2 slope neg.'« and  »TIP2k Filter Papers«


  • Jun-15 Mo
O2k-Open SupportTake care Did you know that fluorescence signal artefacts can occur after the injection of substrates and inhibitors when using the Amplex UltraRed assay?

- » Injection artefact«


  • Jun-22 Mo
O2k-Open SupportTake care Did you know a stirrer test can save your experiments?

Get immediate information on how satisfactory your OroboPOS response time is by performing a stirrer test during air calibration.
- »Oxygen calibration - DatLab«

  • Jun-29 Mo
O2k-Open SupportWarning: loss of data Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?
An 'O2 slope neg.' within ± 2 pmol∙s−1∙mL−1 is optimal (± 4 pmol∙s−1∙mL−1 is acceptable)

- » Air and zero calibration«

Previous O2k-Open Support Alert 2020

Click to expand or collaps

>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

May 2020

  • May-25 Mo
O2k-Open SupportTake care Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?

Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.
- »Switch components approach«

  • May-18 Mo
O2k-Open SupportWarning: loss of data Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?

See how to clean and dry the electrical connections of the OroboPOS-Connector.
- »Cleaning the electrical connections«

  • May-11 Th
O2k-Open SupportTake care Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.

Learn more about steady states including performance and mark setting for data analysis.
- »Steady state«


  • May-04 Mo
O2k-Open SupportTake care Do you load the correct instrumental background correction according to the actual of experimental O2 concentrations?

Perform and apply the instrumental background test.
- » O2 background correction in DatLab« and » Instrumental oxygen background«

Apr 2020

  • Apr-27 Mo
O2k-Open SupportTake care Do you set marks correctly in the instrumental O2 background test when planning to apply the background correction to a SUIT protocol with a high O2 regime?

The range of O2 concentrations selected in the instrumental background test should correspond to the range of experimental O2 concentrations.
- » O2 Background correction - troubleshooting«


  • Apr-20 Tu
O2k-Open SupportImprove Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?

Unstable respiratory fluxes in OXPHOS might be due to substrates limitation.
- » Pyruvate - troubleshooting«


  • Apr-14 Tu
O2k-Open SupportTake care Why are the oxygen levels different between chambers prior to my experimental run?

After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations.
- » Oxygen calibration - troubleshooting«


  • Apr-07 Tu
DatLabImprove Did you know that you can edit the background parameters in the 'O2 analysis template DL7.4?

If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the O2 analysis template DL7.4 for completion of Oxygen flux analysis - DatLab 7.4.
- »SUIT: Browse DL-Protocols and templates«


  • Apr-02 Th
MitoPedia: SUITImprove Do you want to search the best SUIT protocol for your research questions?

- Use the SUITbrowser: »SUITbrowser«

Mar 2020

  • Mar-30 Mo
O2k-Open SupportInfo: improve DatLab 7.4 offers the flexibility to edit a DL-Protocol:

- »Enable DL-Protocol editing«


  • Mar-25 We
O2k-Open SupportInfo: improve O2 flux analysis with DatLab 7.4. Use our latest O2 analysis template DL7.4:

- »O2k-Videosupport: O2 flux analysis with DatLab 7.4«


  • Mar-23 Mo
O2k-Open SupportTake care Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background test when using the O2k-sV-Module?

(1) By adjusting the dithionite concentration to 2.5 mM, the instrumental O2 background test can be performed with the "BG Feedback" settings. - »Dithionite« 
(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module, the instrumental O2 background test can be performed using the O2k-sV-Module. - »O2k-sV-Module Manual«


  • Mar-11 We
O2k-Open SupportInfo: improve Do you change the slope smoothing to 20 while running experiments with biological sample?

While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration.
- »Slope smoothing«


  • Mar-04 We
O2k-Open SupportWarning: loss of data OroboPOS-Holder002.jpg A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.

See how you can check that the two blue OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«

Feb 2020

  • Feb-28 Fr
O2k-Open SupportTake care Calibration parameters are transferred to experimental DatLab files

Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.
- » Oxygen calibration - DatLab«

Jan 2020

  • Jan-31 Fr
O2k-Open SupportTake care Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration

In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP).
- »Oxygen calibration - DatLab«


  • Jan-30 Th
O2k-Open SupportTake care Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?

In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample.
- »Oxygen calibration - DatLab«

O2k-Open SupportInfo: improve Do you set mark ‘R1’ and complete the air calibration correctly?

Your air calibration is not effective until you set mark ‘R1’ and click on Calibrate and copy to clipboard in the O2 calibration window.
- »Oxygen calibration - DatLab«


  • Jan-24 Fr
O2k-Open SupportInfo: improve Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?

Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed.
- »O2k configuration«

O2k-Open SupportWarning: loss of data Do you perform O2k-Chamber volume calibration?

The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module).
- »O2k-Chamber MitoPedia«

O2k-Open SupportTake care Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?

The O2 solubility factor varies according to the medium used and influences the air calibration.
- »Oxygen solubility factor«