Difference between revisions of "Template:Oxygen sensor test"

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[[File:Stirrer test.png|thumb|right|500px|'''Stirrer test''' for quality control (standard 30 s) with 30 min time scale displayed with Graph Layout “02-Calibration - Background” (MiR05; 37 °C; data recording interval: 2 s; slope smoothing: 40 data points).]]
 
[[File:Stirrer test.png|thumb|right|500px|'''Stirrer test''' for quality control (standard 30 s) with 30 min time scale displayed with Graph Layout “02-Calibration - Background” (MiR05; 37 °C; data recording interval: 2 s; slope smoothing: 40 data points).]]
:::# About 20 min are required for approximate air equilibration after temperature equilibration of the incubation medium, visualized as stabilization of the Peltier power (Fig. 2; time scale is 01:10 hh:mm).
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:::# About 20 minutes are required for air equilibration after temperature equilibration of the incubation medium, visualized as stabilization of the Peltier power (Fig. 2; time scale is 01:10 hh:mm).
:::# Even before final equilibration, perform a [[Stirrer_test|stirrer test]] [F9], switching both stirrers automatically off for 30 s.
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:::# Before final equilibration, perform a [[Stirrer_test|stirrer test]] [F9], switching both stirrers automatically off for 30 s.
:::## Quality control a: Upon automatic re-start of the stirrer (On), the increase of the oxygen signal should be rapid and monoexponential (see Fig. right, label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''a'''</span>)
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:::## Quality control label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''a'''</span>: Upon automatic re-start of the stirrer (On), the increase of the oxygen signal should be rapid and monoexponential.
:::## Quality control label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''b'''</span>: The raw signal (blue plot; 1 V = 1 µA at Gain 1) should be close to 1 to 3 V at 25 to 37 °C at sea level up to 1000 m (''p''<sub>b</sub> 101 to 90 kPa). At Gain setting of 2 the raw signal [V] is multiplied by 2.
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:::## Quality control label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''b'''</span>: The raw signal (blue plot; 1 V = 1 µA at Gain 1) should be close to 1 to 3 V at 25 to 37 °C at sea level up to 1000 m (''p''<sub>b</sub> 101 to 90 kPa). At a Gain setting of 2 the raw signal [V] is multiplied by 2.
:::# [[File:O2 calibration air.png|thumb|right|500px|'''Quality control''']] Within 40 min, the oxygen signals should be stable with O2 slope (uncorrected) close to zero.
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:::# [[File:O2 calibration air.png|thumb|right|500px|'''Quality control''']] Within 40 minutes, the oxygen signals should be stable with an O2 slope (uncorrected) close to zero.
:::## Quality control label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''c'''</span>: Signal noise should be low, reflected in a noise of the O2 slope (red plot) within ±2 (±4 is acceptable) pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> at a data recording interval of 2 s and 40 data points selected for calculation of the slope.
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:::## Quality control label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''c'''</span>: Signal noise should be low, reflected in a noise of the O2 slope (red plot) within ± 2 (± 4 is acceptable) pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> at a data recording interval of 2 s and 40 data points selected for calculation of the slope.
 
:::# Set a mark on the oxygen signal (R1) and click on <span style="background:yellow">O2 Calib.</span style> to open the DatLab O2 calibration window.
 
:::# Set a mark on the oxygen signal (R1) and click on <span style="background:yellow">O2 Calib.</span style> to open the DatLab O2 calibration window.
:::## Quality control label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''d'''</span>: The slope uncorrected should be within ±1 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> averaged across the section of the experiment marked as R1 for air calibration (d). The recorded POS signal should be close to the previous calibration under identical experimental conditions. See O2-Calibration window (see Fig. right, label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''b’'''</span>).
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:::## Quality control label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''d'''</span>: The slope uncorrected should be within ± 1 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> averaged across the section of the experiment marked as R1 for air calibration (d). The recorded POS signal should be close to the previous calibration under identical experimental conditions. See O2-Calibration window (see Fig. right, label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''b’'''</span>).
:::# Continue with an [[Oxygen_flux_-_instrumental_background|Instrumental O<sub>2</sub> background test]] (MiPNet14.06) or simply  close the chamber and if required perform a zero oxygen calibration.  
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:::# Continue with an [[Oxygen_flux_-_instrumental_background|Instrumental O<sub>2</sub> background test]] (MiPNet14.06) or simply  close the chamber and if required, perform a zero oxygen calibration.  
 
:::## Quality  control label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''e'''</span>: After closing the chamber, select plot Y2 and  set mark J°1. Background slope (neg.) should be within 2.5 ± 1 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>.
 
:::## Quality  control label <span style="background-color:#FFFF00; border-color:#D4D0D0; border-style: solid; border-width: 1px; margin: 2px">'''e'''</span>: After closing the chamber, select plot Y2 and  set mark J°1. Background slope (neg.) should be within 2.5 ± 1 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup>.
 
:::##:* Flux values higher than 4.0  pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> indicate a biological contamination.
 
:::##:* Flux values higher than 4.0  pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> indicate a biological contamination.

Latest revision as of 09:47, 1 July 2020

The POS sensor test
Stirrer test for quality control (standard 30 s) with 30 min time scale displayed with Graph Layout “02-Calibration - Background” (MiR05; 37 °C; data recording interval: 2 s; slope smoothing: 40 data points).
  1. About 20 minutes are required for air equilibration after temperature equilibration of the incubation medium, visualized as stabilization of the Peltier power (Fig. 2; time scale is 01:10 hh:mm).
  2. Before final equilibration, perform a stirrer test [F9], switching both stirrers automatically off for 30 s.
    1. Quality control label a: Upon automatic re-start of the stirrer (On), the increase of the oxygen signal should be rapid and monoexponential.
    2. Quality control label b: The raw signal (blue plot; 1 V = 1 µA at Gain 1) should be close to 1 to 3 V at 25 to 37 °C at sea level up to 1000 m (pb 101 to 90 kPa). At a Gain setting of 2 the raw signal [V] is multiplied by 2.
  3. Quality control
    Within 40 minutes, the oxygen signals should be stable with an O2 slope (uncorrected) close to zero.
    1. Quality control label c: Signal noise should be low, reflected in a noise of the O2 slope (red plot) within ± 2 (± 4 is acceptable) pmol∙s−1∙mL−1 at a data recording interval of 2 s and 40 data points selected for calculation of the slope.
  4. Set a mark on the oxygen signal (R1) and click on O2 Calib. to open the DatLab O2 calibration window.
    1. Quality control label d: The slope uncorrected should be within ± 1 pmol∙s−1∙mL−1 averaged across the section of the experiment marked as R1 for air calibration (d). The recorded POS signal should be close to the previous calibration under identical experimental conditions. See O2-Calibration window (see Fig. right, label b’).
  5. Continue with an Instrumental O2 background test (MiPNet14.06) or simply close the chamber and if required, perform a zero oxygen calibration.
    1. Quality control label e: After closing the chamber, select plot Y2 and set mark J°1. Background slope (neg.) should be within 2.5 ± 1 pmol∙s−1∙mL−1.
      • Flux values higher than 4.0 pmol∙s−1∙mL−1 indicate a biological contamination.
      • Flux values lower than 1.5 pmol∙s−1∙mL−1:
      1. Air bubbles in the Closed chamber: switch on the illumination of the O2k and inspect the O2k-Chamber through the front window. Remove any air bubbles.
      2. A large volume of medium collected in the receptacle of the stopper: siphon off excess medium.
      3. A larger chamber volume: check O2k-Chamber volume calibration.
    2. Quality control label f: The zero signal at mark R0 for zero calibration should be <2 % of R1 (stable at <5 % is acceptable).