Difference between revisions of "Template:SUIT text D014"

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The SUIT-008 O2 pfi D014 protocol is designed to assess the additivity between the [[NADH_Electron_transfer-pathway_state| N-]] and [[Succinate pathway control state| S-pathway]] in the [[Q-junction]], providing a physiologically relevant estimate of maximum mitochondrial respiratory capacity in permeabilized fibers. It also serves as a diagnostic tool for the activity of the glutamate dehydrogenase and its linked pathways, which could be relevant in some pathologies. SUIT-008 O2 pfi D014 can be easily extended with the CIV assay module.
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The SUIT-008 O2 pfi D014 protocol is designed to assess the additivity between the [[NADH_Electron_transfer-pathway_state| N-]] and [[Succinate pathway control state| S-pathway]] in the [[Q-junction]], providing a physiologically relevant estimate of maximum mitochondrial respiratory capacity in permeabilized fibers. It also serves as a diagnostic tool for the activity of the glutamate dehydrogenase and its linked pathways, which could be relevant in some pathologies. SUIT-008 O2 pfi D014 can be easily extended with the CIV assay module. [[Permeabilized_muscle_fibers#Oxygen_kinetics_of_permeabilized_fibers|Permeabilized muscle fibers]] are sensitive to low oxygen supply due to diffusion restrictions in the fiber preparation that limit the supply of oxygen to the core of the fiber bundle. To counteract this limitation hyperoxic conditions (400-250 µM O<sub>2</sub>) must be employed. To set the optimal oxygen concentration in the O2k-Chamber see [[Setting the oxygen concentration]].

Revision as of 13:25, 30 June 2020

The SUIT-008 O2 pfi D014 protocol is designed to assess the additivity between the N- and S-pathway in the Q-junction, providing a physiologically relevant estimate of maximum mitochondrial respiratory capacity in permeabilized fibers. It also serves as a diagnostic tool for the activity of the glutamate dehydrogenase and its linked pathways, which could be relevant in some pathologies. SUIT-008 O2 pfi D014 can be easily extended with the CIV assay module. Permeabilized muscle fibers are sensitive to low oxygen supply due to diffusion restrictions in the fiber preparation that limit the supply of oxygen to the core of the fiber bundle. To counteract this limitation hyperoxic conditions (400-250 µM O2) must be employed. To set the optimal oxygen concentration in the O2k-Chamber see Setting the oxygen concentration.