Template:SUIT text D014

The SUIT-008 O2 pfi D014 protocol is designed to assess the additivity between the N- and S-pathway in the Q-junction, providing a physiologically relevant estimate of maximum mitochondrial respiratory capacity in permeabilized fibers. It also serves as a diagnostic tool for the activity of the glutamate dehydrogenase and its linked pathways, which could be relevant in some pathologies. SUIT-008 O2 pfi D014 can be easily extended with the CIV assay module. Permeabilized muscle fibers are sensitive to low oxygen supply due to diffusion restrictions in the fiber preparation that limit the supply of oxygen to the core of the fiber bundle. To counteract this limitation hyperoxic conditions (400-250 µM O₂) must be employed. To set the optimal oxygen concentration in the O2k-Chamber see Setting the oxygen concentration.