Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Template OSC Oxygen signal2"

From Bioblast
(Created page with "=== Erzsebet Polyak, University of Wisconsin at Madison === Question: Q1: We have different oxygen levels in chamber A and B. Q2: In chamber A after adding d...")
Β 
Β 
Line 15: Line 15:


A2: The zero voltage in chamber A is at 0.8996V, which is too high. Zero voltage should not exceed 10% (usually is lower than 5%) of that seen in R1. You should do a full [[MiPNet19.18B_POS-service | POS service]].
A2: The zero voltage in chamber A is at 0.8996V, which is too high. Zero voltage should not exceed 10% (usually is lower than 5%) of that seen in R1. You should do a full [[MiPNet19.18B_POS-service | POS service]].
[[Category:Technical service]]

Latest revision as of 10:08, 6 November 2019

Erzsebet Polyak, University of Wisconsin at Madison

Question:

Q1: We have different oxygen levels in chamber A and B.

Q2: In chamber A after adding dithionite the signal does not go down below 100Β΅M.

Different O2 levels and high zero.PNG


Answer:

A1: Different initial oxygen levels after switching on the instrument are due to missing calibration. Sensors need to be air calibrated on each experimental day to account for changes in barometric pressure or to accomodate changes in experimental temperature or medium.

A2: The zero voltage in chamber A is at 0.8996V, which is too high. Zero voltage should not exceed 10% (usually is lower than 5%) of that seen in R1. You should do a full POS service.