Template talk:Next oroboros ecosystem agenda

Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:

 {|
 | logos
 | title in bold <br>
 - link
 |}
 

e.g.,

Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction? - »Instrumental background« and »TIP2k Filter Papers«

See the scheme:

Bioblast alert 2021

Prep for circular:

For Prostate Cancer Bioblast Circular (September?):

Succinate anaplerosis enhances the malignant potential of prostate cancer cells. from the O2k-Network »AT Innsbruck Gnaiger E« and »FR Bordeaux Rossignol R« Bastos Sant'Anna Silva AC, Perez-Valencia JA, Sciacovelli M, Lalou C, Sarlak S, Tronci L, Nikitopoulou E, Meszaros AT, Frezza C, Rossignol R, Gnaiger E, Klocker H (2021) Succinate anaplerosis has an onco-driving potential in prostate cancer cells. Cancers 13:1727.»Bioblast link«

pH-regulated succinate uptake in prostate cancer from the O2k-Network »US PA Philadelphia Orynbayeva Z« Zhunussova A, Sen B, Friedman L, Tuleukhanov S, Brooks AD, Sensenig R, Orynbayeva Z (2015) Tumor microenvironment promotes dicarboxylic acid carrier-mediated transport of succinate to fuel prostate cancer mitochondria. Am J Cancer Res 5:1665-79. »Bioblast link«

Succinate: a key regulator of mitochondrial function and HIF-1α upregulation in prostate cancer from the O2k-Network »AT Innsbruck Oroboros«, »AT Innsbruck Gnaiger E« Weber A, Klocker H, Oberacher H, Gnaiger E, Neuwirt H, Sampson N, Eder IE (2018) Succinate accumulation is associated with a shift of mitochondrial respiratory control and HIF-1α upregulation in PTEN negative prostate cancer cells. Int J Mol Sci 19:2129. »Bioblast link«

Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes from the O2k-Network »AT Innsbruck Gnaiger E« and »AT Innsbruck Oroboros«  Schöpf Bernd, Weissensteiner Hansi, Schäfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Sant’Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. »Bioblast link«

O2k-Publications alert

Author names of O2k-Publications/ Brief for the Agenda vs Bioblast

1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.

Example: Oroboros Ecosystem Agenda example

This means that we have to edit manually the author names within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries)) if they are long or copy from the gray box.

2.Bioblast: SOP: [1] – examples are available using both Surname and First name with long version (e.g., Antunes Diana). If in Bioblast the authors are with short surname in the links please update it to complete surname, guidelines are below.

Guidelines to update a reference

When creating an O2k-Publication/Brief alert, it is the responsibility of the author to check if the reference is accordingly to the correct guidelines:

Correct format for a person called “First Name Surname”:

In the gray box should be “Surname FN” - From where references to be used as O2k-Publication/Brief alerts are copied from.

In the links should be “ Surname First N”

How to edit:

A) Check if all authors with profiles in Bioblast (blue links) have their profiles with full name. If they have, proceed to next step (B). If not, you need to move it following the steps “How to move a profile” below.

B) Click in “Edit with form”.

C) The gray box is edited in “Reference” and the links are changed in “Author(s)”. Change it accordingly and click in “save page” at the end of the page.

How to move a profile (instructions sent by Marija):

So let’s take for example my page: Beno M [2]

As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:

1. Go to e.g. “Beno M” on edit

2. Click on “Move”

3. Change the new title to the full name of the person. In my case it will be Beno M to Beno Marija

4. Now it should look like that:

5. Click on “Move” and leave all the ticked boxes

6.Done

After that you can continue in step B.

If you have any doubt you can contact Cristiane, Marija or Mario.

Next

• Jun 10 Th

Mitoquinone does not alter respiration and mt-membrane potential in obese and diabetic rats. from the O2k-Network »US IA Iowa City Sivitz WI«  Fink BD, Yu L, Coppey L, Obrosov A, Shevalye H, Kerns RJ, Yorek MA, Sivitz WI (2021) Effect of mitoquinone on liver metabolism and steatosis in obese and diabetic rats. Pharmacol Res Perspect 9:e00701. »Bioblast link«

O2k-Open Support alert

• While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.

Next

What is the advantage of using flux control ratios (FCRs)? FCRs provide information on coupling and substrate control of mitochondrial respiration independent of (i) mt-content in cells or tissues, (ii) purification of isolated mitochondrial preparations, and (iii) assay conditions for determination of tissue mass or mt-markers. - »Flux control ratio«

Removed for correction

• Nov-30 Mo

Noisy oxygen signal? Check the OroboPOS and OroboPOS-Connector to clean electrical connections. Contaminating grease, salt crystals and moisture can be removed with a dry paper cloth or a paper cloth moistened with water and then absolute ethanol. - »Cleaning the electrical connections«  - communicated by »Schmitt Sabine« and »Komlodi Timea«

WGT recommended not to use water for cleaning. Erich introduced water for cleaning to remove salt crystals.

Do you perform the leaky chamber test after chamber assembly? An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly. - »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«

O2k-Open Support alert circulars

Back to basics:

Do you know how to apply the air and zero calibration values in DatLab? This important quality control step ensures accurate measurement of respiration. For details on how to apply air and zero calibration in DatLab, see: »Oxygen calibration - DatLab« and »Troubleshooting«  - communicated by »Komlodi Timea« and »Cecatto Cristiane«

O2k-checklist: Get started with an O2k-experiment The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment. - »MiPNet20.04_O2k-checklist«  - communicated by »Cecatto Cristiane« and »Schmitt Sabine«

Do you enter the correct instrumental background correction in DatLab for the experimental O2 concentrations? After performing the instrumental background test at the appropriate O2 range, load the parameters in DatLab to ensure accurate correction for external fluxes. - »O2 background correction in DatLab« - communicated by »Komlodi Timea« and »Cecatto Cristiane«

Maybe?

Do you know how to properly insert the ISS-tip into the O2k-chamber? With the stirrer bar still rotating, insert the ISS-tip to the bottom of the O2k-Chamber, so that it points away from you. - »MiPNet19.03 O2k-cleaning and ISS: Section 2«  - communicated by »Doerrier Carolina«, »Cecatto Cristiane« and »Komlodi Timea«

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)

Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: Proven -80 °C solid stability for more than 3 years (3 years 7 months) Proven -20 °C solid stability for 1 year Proven DMSO stability at room temperature for 1 week Proven DMSO stability at -20 °C for 6 months (ongoing)

2021

• 2021-01-06 https://www.jstor.org/stable/1706346?seq=1

Postponed events

ASMRM 2020 Singapore SG

MitoEAGLE Summit Obergurgl 2020

MiPschool Obergurgl 2020

San Diego CA US

• Abstracts deadline 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology

Bioenergetics Vienna 2020

IOC Schroecken AT

»36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ« (Sep 10-12)