Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Template talk:Next oroboros ecosystem agenda"

From Bioblast
Line 105: Line 105:
SUIT-10 was designed to determine the optimum digitonin concentration for permeabilization of the cell plasma membrane in experiments with the O2k.
SUIT-10 was designed to determine the optimum digitonin concentration for permeabilization of the cell plasma membrane in experiments with the O2k.
<br>
<br>
- »'''[[Digitonin|Optimum Digitonin concentration]]''' and '''[[SUIT-10|SUIT-010]]'''«<br>
- »'''[[Digitonin|Optimum Digitonin concentration]]''' and '''[[SUIT-010|SUIT-010]]'''«<br>
- communicated by »[[Schmitt Sabine]]« and »[[Cecatto Cristiane]]«
- communicated by »[[Schmitt Sabine]]« and »[[Cecatto Cristiane]]«
|}
|}
Line 115: Line 115:
SUIT-10 was designed to determine the optimum digitonin concentration for permeabilization of the cell plasma membrane without damaging mitochondrial membranes.
SUIT-10 was designed to determine the optimum digitonin concentration for permeabilization of the cell plasma membrane without damaging mitochondrial membranes.
<br>
<br>
- »'''[[Digitonin|Optimum Digitonin concentration]]''' and '''[[SUIT-10|SUIT-010]]'''«<br>
- »'''[[Digitonin|Optimum Digitonin concentration]]''' and '''[[SUIT-010|SUIT-010]]'''«<br>
- communicated by »[[Schmitt Sabine]]« and »[[Cecatto Cristiane]]«
- communicated by »[[Schmitt Sabine]]« and »[[Cecatto Cristiane]]«



Revision as of 09:36, 4 May 2022

Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
 {|
 | logos
 | title in bold <br>
 - link
 |}
 

e.g.,

O2k-Open SupportWarning: loss of data Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?

- »Instrumental background« and »TIP2k Filter Papers«

See the scheme:


Agenda/Alerts body text format

Bioblast alert 2021

Prep for circular:

MitoPedia alert

Next

O2k-Brief alert

Next

O2k-Publications alert

Author names of O2k-Publications/ Brief for the Agenda vs Bioblast

1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
Example: Oroboros Ecosystem Agenda example
This means that we have to edit manually the author names within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries)) if they are long or copy from the gray box.
2.Bioblast: SOP: [1] – examples are available using both Surname and First name with long version (e.g., Antunes Diana). If in Bioblast the authors are with short surname in the links please update it to complete surname, guidelines are below.
Click to expand or collaps

Guidelines to update a reference

When creating an O2k-Publication/Brief alert, it is the responsibility of the author to check if the reference is accordingly to the correct guidelines:

Reference example.png

Correct format for a person called “First Name Surname”:
In the gray box should be “Surname FN” - From where references to be used as O2k-Publication/Brief alerts are copied from.

Gray box reference.png

In the links should be “ Surname First N”

Authors names links reference.png

How to edit:
A) Check if all authors with profiles in Bioblast (blue links) have their profiles with full name. If they have, proceed to next step (B). If not, you need to move it following the steps “How to move a profile” below.
B) Click in “Edit with form”.
C) The gray box is edited in “Reference” and the links are changed in “Author(s)”. Change it accordingly and click in “save page” at the end of the page.

Editing reference.png

How to move a profile (instructions sent by Marija):

So let’s take for example my page: Beno M [2]

As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
1. Go to e.g. “Beno M” on edit
2. Click on “Move”
3. Change the new title to the full name of the person. In my case it will be Beno M to Beno Marija

BenoMreference.jpg

4. Now it should look like that:

BenoMarijaReference.png

5. Click on “Move” and leave all the ticked boxes
6.Done
After that you can continue in step B.
If you have any doubt you can contact Cristiane, Marija or Mario.

Next

in preparation
Once available (and if open access) promote the FAT4BRAIN publication:Acylcarnitines: Nomenclature, biomarkers, therapeutic potential, drug targets and clinical trials (Dambrova M, Makrecka-Kuka M, Kuka J, Vilskersts R, Nordberg D, Attwood MM, Smesny S, Duygu Sen Z, Guo AC, Oler E, Tian S, Zheng J, Wishart DS, Liepinsh E, Schiöth HB in Pharmacological Reviews


O2k-Publications in the MiPMap "text [text]"
from the O2k-Network »link« and »link«

O2k-Open Support alert

  • While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.

Next

  • May-xx
O2k-Open SupportInfo: improve Do you know which digitonin concentration is needed for permeabilization of the cell plasma membrane without damaging mitochondrial membranes?

SUIT-10 was designed to determine the optimum digitonin concentration for permeabilization of the cell plasma membrane in experiments with the O2k.
- »Optimum Digitonin concentration and SUIT-010«
- communicated by »Schmitt Sabine« and »Cecatto Cristiane«

  • May-xx
O2k-Open SupportInfo: improve Do you know which digitonin concentration is needed for permeabilization of the cell plasma membrane when performing experiments with the O2k?

SUIT-10 was designed to determine the optimum digitonin concentration for permeabilization of the cell plasma membrane without damaging mitochondrial membranes.
- »Optimum Digitonin concentration and SUIT-010«
- communicated by »Schmitt Sabine« and »Cecatto Cristiane«

This one needs an update of the Bioblast page and clarification of MiPNet06.03

  • month-xx
O2k-Open SupportInfo: improve Did you enter the correct oxygen solubility factor in DatLab?

The oxygen solubility factor of the incubation medium, FM, expresses the effect of the salt concentration on oxygen solubility relative to pure water. The correct oxygen solubility factor of your experimental medium or buffer needs to be entered in DatLab for proper calculation of the oxygen concentration.
- »Oxygen solubility factor«
- communicated by »Schmitt Sabine« and »[[ ]]«

This one needs a BioBlast link:

  • Month-##
O2k-Open SupportInfo: improve When filling in the volume of uncoupler in the DatLab mark or the excel template, should one enter the total volume added or the volume to get to the highest flux?

The volume added until the mark, equal to the highest flux, should be entered. Anything titrated after this mark should be summed to the other volumes before the next mark because this will be considered to calculate the sample dilution.
- »LINK and O2 flux analysis with DatLab 7.4«
- communicated by »Cardoso Luiza« and »[[ ]]«

Under discussion

Removed for correction

  • Nov-30 Mo
O2k-Open SupportInfo: improve Noisy oxygen signal? Check the OroboPOS and OroboPOS-Connector to clean electrical connections.

Contaminating grease, salt crystals and moisture can be removed with a dry paper cloth or a paper cloth moistened with water and then absolute ethanol.
- »Cleaning the electrical connections« 
- communicated by »Schmitt Sabine« and »Komlodi Timea«

WGT recommended not to use water for cleaning. Erich introduced water for cleaning to remove salt crystals.


O2k-Open SupportWarning: loss of data Do you perform the leaky chamber test after chamber assembly?

An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«

O2k-Open Support alert circulars

In preparation

MitoFit pdf From preprint to journal publication:

Fischer C, Valente de Souza L, Komlódi T, Garcia-Souza LF, Volani C, Tymoszuk P, Demetz E, Seifert M, Auer K, Hilbe R, Brigo N, Petzer V, Asshoff M, Gnaiger E, Weiss G (2022) Mitochondrial respiration in response to iron deficiency anemia. Comparison of peripheral blood mononuclear cells and liver. Metabolites 12:270. https://doi.org/10.3390/metabo12030270

from the O2k-Network »AT Innsbruck Oroboros«
  • ###-## Tu
Bioblast2022 logo.png
Alena Zikova will be presenting at Bioblast 2022 on Parasites - the mitochondrial outliers.
Bioblast 2022« , »Zikova Alena«









MitoFit Preprints

  • ###-## Tu
Bioenergetics Communications From preprint to BEC manuscript with published Open Peer Reviews:
  • Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. doi:10.26124/BEC:2021-0004
from the O2k-Network »AT Innsbruck Oroboros«

Data analysis templates and MipNets

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
MitoPedia: SUIT Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
  • Proven -80 °C solid stability for more than 3 years (3 years 7 months)
  • Proven -20 °C solid stability for 1 year
  • Proven DMSO stability at room temperature for 1 week
  • Proven DMSO stability at -20 °C for 6 months (ongoing)

2021

Postponed events

link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRMASMRM 2020 Singapore SG
COST Action MitoEAGLE MitoEAGLE Summit Obergurgl 2020
COST Action MitoEAGLE MiPschool Obergurgl 2020


O2k-Workshops San Diego CA US
Bioenergetics Vienna 2020.jpg Bioenergetics Vienna 2020
O2k-Workshops IOC Schroecken AT


»36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ« (Sep 10-12)