Difference between revisions of "Theall 2021 Physiol Rep"
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|keywords=PBMC, Acute exercise, Lymphocyte activation, Oxidative phosphorylation, Respiration | |keywords=PBMC, Acute exercise, Lymphocyte activation, Oxidative phosphorylation, Respiration | ||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
|mipnetlab=US LA Baton Rouge Irving BA | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Exercise physiology;nutrition;life style | ||
|instruments=Oxygraph-2k | |organism=Human | ||
|additional=2021-12 | |tissues=Blood cells | ||
|preparations=Permeabilized cells, Intact cells | |||
|couplingstates=LEAK, ROUTINE, OXPHOS, ET | |||
|pathways=N, NS, ROX | |||
|instruments=Oxygraph-2k, O2k-Fluorometer | |||
|additional=2021-12, AmR, PBMCs | |||
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Latest revision as of 18:35, 15 December 2021
| Theall B, Stampley J, Cho E, Granger J, Johannsen NM, Irving BA, Spielmann G (2021) Impact of acute exercise on peripheral blood mononuclear cells nutrient sensing and mitochondrial oxidative capacity in healthy young adults. Physiol Rep 9:e15147. |
Theall Bailey, Stampley James, Cho Eunhan, Granger Joshua, Johannsen Neil M, Irving Brian A, Spielmann Guillaume (2021) Physiol Rep
Abstract: Regular exercise is associated with changes in peripheral blood mononuclear cell (PBMC) proportions that have enhanced effector functions in young and old adults; however, the effects of acute exercise on PBMC nutrient sensors and metabolic function in active young adults is unknown. To fill this gap, activation status and nutrient-sensing mechanisms of PBMCs isolated from 21 healthy active adults (20-35 yr; 36.5 ยฑ 6.3 VฬO2peak ) were characterized before and after 30 min of moderate-to-vigorous cycling (65%-75% VฬO2peak ). In addition, changes in PBMC mitochondrial respiratory function in response to exercise were assessed using high-resolution respirometry. There was an increase in the number of activated CD69+/CD4 (79% increase) and CD69+/CD8 (166% increase) T-cells in response to the acute bout of exercise, while the nutrient-sensing mechanisms remained unchanged. PBMC mitochondrial respiration did not increase on a cell-per-cell basis, however, mitochondrial oxidative capacity (OXPHOS) increased at the tissue level (18.6 pmol/(s*ml blood) versus 29.3 pmol/(s*ml blood); p < 0.05) in response to acute exercise. Thus, this study shows that acute exercise preferentially mobilizes activated T-cells while concomitantly increasing PBMC mitochondrial oxidative capacity at the tissue level, rather than acutely changing mitochondrial oxidative capacity at the cellular level in young adults. โข Keywords: PBMC, Acute exercise, Lymphocyte activation, Oxidative phosphorylation, Respiration โข Bioblast editor: Plangger M โข O2k-Network Lab: US LA Baton Rouge Irving BA
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Blood cells
Preparation: Permeabilized cells, Intact cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, NS, ROX
HRR: Oxygraph-2k, O2k-Fluorometer
2021-12, AmR, PBMCs
