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Difference between revisions of "Tissue homogenate"

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Homogenized tissue provides different advantages: the preparation is faster, no detergents (saponin) are required, tissue heterogeneity may entail a statistical problem in application of fibres which is averaged in the homogenate preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compared to isolated mitochondria. Tissue homogenates may be well suited for the study of mitochondrial respiration [[(Pecinova 2011 Mitochondrion)]].
Homogenized tissue provides different advantages: the preparation is faster, no detergents (saponin) are required, tissue heterogeneity may entail a statistical problem in application of fibres which is averaged in the homogenate preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compared to isolated mitochondria. Tissue homogenates may be well suited for the study of mitochondrial respiration ([[Pecinova 2011 Mitochondrion]]).

Revision as of 11:43, 21 May 2012


high-resolution terminology - matching measurements at high-resolution


Tissue homogenate

Description

A tissue homogenate is obtained through mechanical micro-disruption of fresh tissue.


Reference: MiPNet17.02 PBI-Shredder-Manual



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Preparation: Tissue homogenate"Tissue homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 





Homogenized tissue provides different advantages: the preparation is faster, no detergents (saponin) are required, tissue heterogeneity may entail a statistical problem in application of fibres which is averaged in the homogenate preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compared to isolated mitochondria. Tissue homogenates may be well suited for the study of mitochondrial respiration (Pecinova 2011 Mitochondrion).