Difference between revisions of "User talk:Spitzer Gudrun"

Latest revision as of 12:12, 23 October 2020

Antimycin A version 1

Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540

Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 5 mM stock solution (dissolved in 99.9% ethanol) for 2-mL O2k-chamber:

Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
Add 2 mL ethanol.
Divide into 0.2 mL portions in clear glass vials.
Store at -20 °C.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
Final concentration: 2.5 µM.

Preparation of 1,25 mM stock solution (dissolved in 99.9% ethanol) for 0.5-mL O2k-chamber:

Take 100 µL of 5 mM stock solution.
Add 300 µL ethanol.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
Final concentration: 2.5 µM.

Antimycin A version 2

Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540

Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):

Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
Add 2 mL ethanol.
Divide into 0.2 mL portions in clear glass vials.
Store at -20 °C.
For 1.25 mM concentration take 100 µL stock solution (5 mM) and dilute it with 300 µl ethanol.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 1 µL (5 mM) using a 10 µL Hamilton syringe.
Titration volume (0.5-mL O2k-chamber): 1 µL (1.25 mM) using a 10 µL Hamilton syringe.
Final concentration: 2.5 µM.

@@ Line 1: / Line 1: @@
-== Asc gutest 1==
+== Antimycin A version 1 ==
-:::: '''As: Ascorbate sodium salt''' (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
+::: '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: FW = 540
-:::: <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial).</span>
+:::: <span style="color:#8B008B"> '''Caution:''' '''Very toxic.''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
-'
-::: '''Preparation of 800 mM stock solution''' (dissolved in H<sub>2</sub>O) for use in '''2-mL O2k-chamber''':
-::::# Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H<sub>2</sub>O
-::::# To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL<sup>-1</sup> solution of pH 2).
-::::# Divide into 0.2 mL portions.
-::::# Store frozen at -20 °C.
-::: '''Preparation of 1000 mM stock solution''' (dissolved in H<sub>2</sub>O) for use in '''0.5-mL O2k-chamber''':
+::: '''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol) for '''2-mL O2k-chamber''':
-::::# Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H<sub>2</sub>O
+::::# Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
-::::# To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL<sup>-1</sup> solution of pH 2).
+::::# Add 2 mL ethanol.
-::::# Divide into 0.2 mL portions.
+::::# Divide into 0.2 mL portions in clear glass vials.
-::::# Store frozen at -20 °C.
+::::# Store at -20 °C.
+:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-:::»'''O2k manual titrations:''' [[MiPNet09.12]]
+::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
+::::* Final concentration: 2.5 µM.
-::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL of 800mM stock solution using a 25 µL Hamilton syringe.
-::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 1000mM stock solution using a 10 µL Hamilton syringe
-::::* Final concentration: 2 mM.
-== Asc gutest 2 ==
+::: '''Preparation of 1,25 mM stock solution''' (dissolved in 99.9% ethanol) for '''0.5-mL O2k-chamber''':
-:::: '''As: Ascorbate sodium salt''' (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
+::::# Take 100 µL of 5 mM stock solution.
+::::# Add 300 µL ethanol.
-:::: <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial).</span>
+:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::: '''Preparation of 800 mM stock solution''' (dissolved in H<sub>2</sub>O) for use in '''2-mL O2k-chamber''':
-::::# Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H<sub>2</sub>O
-::::# To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL<sup>-1</sup> solution of pH 2).
-::::# Divide into 0.2 mL portions.
-::::# Store frozen at -20 °C.
-:::»'''O2k manual titrations:''' [[MiPNet09.12]]
-::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL of 800 mM stock solution using a 25 µL Hamilton syringe.
-::::* Final concentration: 2 mM.
-::: '''Preparation of 1000 mM stock solution''' (dissolved in H<sub>2</sub>O) for use in '''0.5-mL O2k-chamber''':
-::::# Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H<sub>2</sub>O
-::::# To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL<sup>-1</sup> solution of pH 2).
-::::# Divide into 0.2 mL portions.
-::::# Store frozen at -20 °C.
-:::»'''O2k manual titrations:''' [[MiPNet09.12]]
-::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 1000 mM stock solution using a 10 µL Hamilton syringe.
-::::* Final concentration: 2 mM.
-== Version 3 ==
-:::: '''As: Ascorbate sodium salt''' (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
-:::: <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial).</span>
+::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
+::::* Final concentration: 2.5 µM.
-:::: '''Preparation of 800 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''2-mL O2k-chamber''':
+== Antimycin A version 2 ==
-::::# Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H<sub>2</sub>O
+::: '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: FW = 540
-::::# To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL<sup>-1</sup> solution of pH 2).
-::::# Divide into 0.2 mL portions.
-::::# Store frozen at -20 °C.
-:::»'''O2k manual titrations:''' [[MiPNet09.12]]
+:::: <span style="color:#8B008B"> '''Caution:''' '''Very toxic.''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
-::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe.
-::::* Final concentration: 2 mM.
-:::: '''Preparation of 1000 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''0.5-mL O2k-chamber''':
-::::# Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H<sub>2</sub>O
-::::# To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL<sup>-1</sup> solution of pH 2).
-::::# Divide into 0.2 mL portions.
-::::# Store frozen at -20 °C.
-:::»'''O2k manual titrations:''' [[MiPNet09.12]]
-::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
-::::* Final concentration: 2 mM.
-== O2k manual titrations - list of chemicals ==
-=== same conc for both chambers ===
+::: '''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol):
-:::: This text is a test
+::::# Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
-:::: And even more words
+::::# Add 2 mL ethanol.
+::::# Divide into 0.2 mL portions in clear glass vials.
+::::# Store at -20 °C.
+::::# For '''1.25''' mM concentration take 100 µL stock solution (5 mM) and dilute it with 300 µl ethanol.
-=== diff conc for sV and lV chamber ===
+:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::::* Another test
+::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL (5 mM) using a 10 µL Hamilton syringe.
-::::* Third line of the test
+::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL (1.25 mM) using a 10 µL Hamilton syringe.
+::::* Final concentration: 2.5 µM.