Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Vinnakota 2016a Biophys J

From Bioblast
Revision as of 15:29, 8 April 2020 by Gnaiger Erich (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision β†’ (diff)
Publications in the MiPMap
Vinnakota KC, Bazil JN, Van den Bergh F, Wiseman RW, Beard DA (2016) Feedback regulation and time hierarchy of oxidative phosphorylation in cardiac mitochondria. Biophys J 110:972-80.

Β» PMID: 26910434 Open Access

Vinnakota KC, Bazil JN, Van den Bergh F, Wiseman RW, Beard DA (2016) Biophys J

Abstract: To determine how oxidative ATP synthesis is regulated in the heart, the responses of cardiac mitochondria oxidizing pyruvate to alterations in [ATP], [ADP], and inorganic phosphate ([Pi]) were characterized over a range of steady-state levels of extramitochondrial [ATP], [ADP], and [Pi]. Evolution of the steady states of the measured variables with the flux of respiration shows that: (1) a higher phosphorylation potential is achieved by mitochondria at higher [Pi] for a given flux of respiration; (2) the time hierarchy of oxidative phosphorylation is given by phosphorylation subsystem, electron transport chain, and substrate dehydrogenation subsystems listed in increasing order of their response times; (3) the matrix ATP hydrolysis mass action ratio [ADP] Γ— [Pi]/[ATP] provides feedback to the substrate dehydrogenation flux over the entire range of respiratory flux examined in this study; and finally, (4) contrary to previous models of regulation of oxidative phosphorylation, [Pi] does not modulate the activity of complex III.

Copyright Β© 2016 Biophysical Society. Published by Elsevier Inc. All rights reserved. β€’ Keywords: Decylubiquinone

β€’ O2k-Network Lab: US MI Ann Arbor Beard DA, US MI East Lansing Bazil JN


Labels: MiParea: Respiration 


Organism: Rat  Tissue;cell: Heart  Preparation: Isolated mitochondria 

Regulation: ADP, ATP, Flux control, Phosphate  Coupling state: LEAK, OXPHOS  Pathway:HRR: Oxygraph-2k, TPP 

2016-03