Difference between revisions of "O2k-Virtual Support self-study material"

Revision as of 15:47, 27 April 2021

O2k-Virtual Support self-study material

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

General information

To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with Materials for self-study in advance.

O2k-Basic

Part 1.1: OroboPOS service and O2k instrumental

Part 1.2: DatLab

Part 1.3: O₂ calibration and instrumental background

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

Sample types,mitochondrial preparations and sample addition:

SUIT protocols, SUITbrowser, DL-Protocols:

Part 2.2: Biological experiment and data analysis

O2k-Advanced

O2k-Applications: Simultaneous determination of O₂ and H₂O₂ fluxes

NextGen-O2k KOL Training

PB-Module Manual (R)

O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R)

DL-Protocol: SUIT-030_PB_ce_D069 (R)
O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R)

DL-Protocol: SUIT-030_PB_ce_D070 (R)

Q-Module

Q-Module manual: MiPNet24.12 NextGen-O2k: Q-Module
CV software manual: MiPNet24.16 DatLab8.0: CV-Module
Videos: Q-Module

@@ Line 4: / Line 4: @@
 == General information ==
-:::: To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with '''Materials for self-study''' in advance. These materials are either Open Access (OA) or will be sent upon registration (R).
+:::: To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with '''Materials for self-study''' in advance.
 == O2k-Basic ==
 === Part 1.1: OroboPOS service and O2k instrumental ===
-:::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] (OA)
+:::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]]
-:::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] (OA)
+:::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]]
-:::* [https://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS] (OA)
+:::* [https://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS]
-:::* [https://www.youtube.com/watch?v=HC6MLXO08k8&feature=youtu.be O2k-Videosupport: Cathode cleaning] (OA)
+:::* [https://www.youtube.com/watch?v=HC6MLXO08k8&feature=youtu.be O2k-Videosupport: Cathode cleaning]
-:::* [https://www.youtube.com/watch?v=sDoU69-SLq0&feature=youtu.be O2k-Videosupport: Anode cleaning] (OA)
+:::* [https://www.youtube.com/watch?v=sDoU69-SLq0&feature=youtu.be O2k-Videosupport: Anode cleaning]
-:::* [https://www.youtube.com/watch?v=iGafaPa4OyA&feature=youtu.be O2k-Videosupport: Membrane mounting] (OA)
+:::* [https://www.youtube.com/watch?v=iGafaPa4OyA&feature=youtu.be O2k-Videosupport: Membrane mounting]
-:::* [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 O2k-Videosupport: Start DatLab 7] (OA)
+:::* [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 O2k-Videosupport: Start DatLab 7]
-:::* [https://www.youtube.com/watch?v=pGzb0KfYwTQ&feature=youtu.be O2k-Videosupport: Insert OroboPOS] (OA)
+:::* [https://www.youtube.com/watch?v=pGzb0KfYwTQ&feature=youtu.be O2k-Videosupport: Insert OroboPOS]
-:::* [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber] (OA)
+:::* [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber]
-:::* [https://www.youtube.com/watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration] (OA)
+:::* [https://www.youtube.com/watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration]
 === Part 1.2: DatLab ===
-:::* [[MitoPedia: DatLab]] (OA)
+:::* [[MitoPedia: DatLab]]
-:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]] (OA)
+:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]]
 === Part 1.3: O<sub>2</sub> calibration and instrumental background  ===
-:::* [[Gnaiger 2008 POS]] (OA)
+:::* [[Gnaiger 2008 POS]]
-:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]] (OA)
+:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]]
-:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]] (OA)
+:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]]
-:::* O2k-Videosupport: QC1: POS calibration (R)
+:::* [https://www.youtube.com/watch?v=onOIVpmOEoE O2k-Videosupport: QC1: POS calibration]
-:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]] (OA)
+:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]]
 === Part 2.1: Biological samples and experimental design ===
 '''Pathway control and coupling control of mitochondrial respiration:'''
-:::* [[Gnaiger 2020 BEC MitoPathways|BEC 2020.2 Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis]] (OA)
+:::* [[Gnaiger 2020 BEC MitoPathways|BEC 2020.2 Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis]]
-:::* [[BEC 2020.1 doi10.26124bec2020-0001.v1|BEC 2020.1 Mitochondrial Physiology]] (OA)
+:::* [[BEC 2020.1 doi10.26124bec2020-0001.v1|BEC 2020.1 Mitochondrial Physiology]]
 '''Sample types,mitochondrial preparations and sample addition:'''
-:::* [[Sample]] (OA)
+:::* [[Sample]]
-:::* [[Mitochondrial preparations]] (OA)
+:::* [[Mitochondrial preparations]]
-:::* [[MiPNet17.03 Shredder vs Fibres]] (OA)
+:::* [[MiPNet17.03 Shredder vs Fibres]]
-:::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate] (OA)
+:::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate]
-:::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization] (OA)
+:::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization]
-:::* [[Cell count and normalization in HRR]] (OA)
+:::* [[Cell count and normalization in HRR]]
-:::* O2k-Videosupport: Addition of biological sample to the respirometer chamber (R)
+:::* [https://www.youtube.com/watch?v=GX_SvnVGXgg O2k-Videosupport: Addition of biological sample to the respirometer chamber]
 '''SUIT protocols, SUITbrowser, DL-Protocols:'''
-:::* [[MitoPedia: SUIT]] (OA)
+:::* [[MitoPedia: SUIT]]
-:::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser] (OA)
+:::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser]
-:::* [https://www.youtube.com/watch?v=8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)] (OA)
+:::* [https://www.youtube.com/watch?v=8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)]
-:::* [https://www.youtube.com/watch?v=Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)] (OA)
+:::* [https://www.youtube.com/watch?v=Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)]
 === Part 2.2: Biological experiment and data analysis ===
-:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen_flux_analysis]] (OA)
+:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen_flux_analysis]]
-:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4] (OA)
+:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]
 == O2k-Advanced ==
 ''' O2k-Applications: Simultaneous  determination  of  O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes'''
-:::* [[Amplex UltraRed]] (OA)
+:::* [[Amplex UltraRed]]
-:::* O2k-Videosupport: Smart Fluo-Sensor assembly (R)
+:::* [https://www.youtube.com/watch?v=nlVLXcbBRqs O2k-Videosupport: Smart Fluo-Sensor assembly]
-:::* O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor (R)
+:::* [https://www.youtube.com/watch?v=eUukvgAi1yY O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor]
-:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]] (OA)
+:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]]
-:::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]] (OA)
+:::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]]
-:::* O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4 (R)
+:::* [https://www.youtube.com/watch?v=_Fv0VSA_c14 O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
@@ Line 75: / Line 75: @@
 :::* '''Q-Module'''
-::::* Q-Module manual: [[MiPNet24.12 NextGen-O2k: Q-Module]] (OA)
+::::* Q-Module manual: [[MiPNet24.12 NextGen-O2k: Q-Module]]
-::::* CV software manual: [[MiPNet24.16 DatLab8.0: CV-Module]] (OA)
+::::* CV software manual: [[MiPNet24.16 DatLab8.0: CV-Module]]
-::::*Videos: [https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 Q-Module] (OA)
+::::*Videos: [https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 Q-Module]