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Wisniewski 1995 Eur J Biochem

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Wisniewski E, Gellerich FN, Kunz WS (1995) Distribution of flux control among the enzymes of mitochondrial oxidative phosphorylation in calcium-activated saponin-skinned rat musculus soleus fibers. Eur J Biochem 230: 549-554.

Β» PMID: 7607228

Wisniewski E, Gellerich FN, Kunz WS (1995) Eur. J. Biochem.

Abstract: Metabolic control analysis was applied to describe the control of mitochondrial oxidative phosphorylation in calcium (approximately 2 pM free calcium) activated saponin-skinned rat musculus soleus fibers oxidizing glutamate and malate. Under these circumstances approximately 80 % of mitochondrial activestate respiration was reached due to the activation of ATP turnover by actomyosin ATPase. The flux control coefficients of H+-ATPase, adenine-nucleotide translocase, phosphate transporter, NADH :ubiquinone oxidoreductase and cytochrome-c oxidase were determined to be equal to 0.16 2 0.08 (n = 6), 0.34? 0.12 (n = 5), 0.08 2 0.03 (n = 5), 0.01 -+ 0.006 (n = 4) and 0.09 ? 0.03 (n = 3) using inhibitor titrations with the specific inhibitors oligomycin, carboxyatractyloside, mersalyl, rotenone and cyanide, respectively, and applying non-linear regression of the entire titration curve. The flux control coefficient of actomyosin ATPase was determined with vanadate to be equal to 0.50?0.09 (n = 6), measuring independently the vanadate-caused inhibition of fiber respiration and ATP-splitting activity. In contrast to results with isolated rat skeletal muscle mitochondria reconstituted with soluble F,-ATPase the decrease in phosphate concentration from 10 mM to 1 mM only slightly affected the distribution of flux control coefficients. This difference is caused by different kinetic properties of soluble F,-ATPase and actomyosin ATPase. Therefore, phosphate seems to be in skeletal muscle in vivo only a modest modulator of control of oxidative phosphorylation. β€’ Keywords: Skinned muscle fibers, Flux control, Oxidative phosphorylation


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Organism: Rat  Tissue;cell: Skeletal Muscle"Skeletal Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 



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