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Zoll 2002 J Physiol

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Publications in the MiPMap
Zoll J, Sanchez H, N'Guessan B, Ribera F, Lampert E, Bigard X, Surrurier B, Fortin D, Geny B, Veksler V, Ventura-Clapier R, Mettauer B (2002) Physical activity changes the regulation of mitochondrial respiration in human skeletal muscle. J Physiol 543:191-200.

» PMID: 12181291 Open Access

Zoll J, Sanchez H, N'Guessan B, Ribera F, Lampert E, Bigard X, Surrurier B, Fortin D, Geny B, Veksler V, Ventura-Clapier R, Mettauer B (2002) J Physiol

Abstract: This study explores the importance of creatine kinase (CK) in the regulation of muscle mitochondrial respiration in human subjects depending on their level of physical activity. Volunteers were classified as sedentary, active or athletic according to the total activity index as determined by the Baecke questionnaire in combination with maximal oxygen uptake values (peak V(O2), expressed in ml min(-1) kg(-1)). All volunteers underwent a cyclo-ergometric incremental exercise test to estimate their peak V(O2) and V(O2) at the ventilatory threshold (VT). Muscle biopsy samples were taken from the vastus lateralis and mitochondrial respiration was evaluated in an oxygraph cell on saponin permeabilised muscle fibres in the absence (V(0)) or in the presence (V(max)) of saturating [ADP]. While V(0) was similar, V(max) differed among groups (sedentary, 3.7 +/- 0.3, active, 5.9 +/- 0.9 and athletic, 7.9 +/- 0.5 micromol O2 min(-1) (g dry weight)(-1)). V(max) was correlated with peak V(O2) (P < 0.01, r = 0.63) and with V(T) (P < 0.01, r = 0.57). There was a significantly greater degree of coupling between oxidation and phosphorylation (V(max)/V(0)) in the athletic individuals. The mitochondrial K(m) for ADP was significantly higher in athletic subjects (P < 0.01). Mitochondrial CK (mi-CK) activation by addition of creatine induced a marked decrease in K(m) in athletic individuals only, indicative of an efficient coupling of mi-CK to ADP rephosphorylation in the athletic subjects only. It is suggested that increasing aerobic performance requires an enhancement of both muscle oxidative capacity and mechanisms of respiratory control, attesting to the importance of temporal co-ordination of energy fluxes by CK for higher efficacy.

• Bioblast editor: Gnaiger E

MitoEAGLE VO2max/BME database

  • Human vastus lateralis
  • 2 females & 11 males
  • 40.5 years
  • Athletic; involved in a training programme with endurance sessions on a regular basis
  • H = 1.74 m
  • M = 68.2 kg
  • BME = 0.11
  • BMI = 22.5 kg·m-2
  • VO2max/M = 53.9 mL·min-1·kg-1 (= VO2peak/M/0.93)
  • Permeabilized muscle fibres; 22 °C; GMP; md; conversions: Gnaiger 2009 Int J Biochem Cell Biol
  • JO2,P(NS) = 145.8 µmol·s-1·kg-1 wet muscle mass (37 °C)
  • JO2,P(GM) = 106.4 µmol·s-1·kg-1 wet muscle mass (37 °C)
  • JO2,P(NS) = JO2,P(GM)/0.73
  • Fiber wet mass to dry mass ratio = 3.5 (N'Guessan 2004 Mol Cell Biochem)
  • Human vastus lateralis
  • 2 females & 6 males
  • 45.6 years
  • Active; performed physical activity regularly for professional or recreational purposes, but followed no regular training programme
  • H = 1.73 m
  • M = 78.1 kg
  • BME = 0.29
  • BMI = 26.1 kg·m-2
  • VO2max/M = 39.0 mL·min-1·kg-1 (= VO2peak/M/0.93)
  • Permeabilized muscle fibres; 22 °C; GMP; md; conversions: Gnaiger 2009 Int J Biochem Cell Biol
  • JO2,P(NS) = 108.9 µmol·s-1·kg-1 wet muscle mass (37 °C)
  • JO2,P(GM) = 79.5 µmol·s-1·kg-1 wet muscle mass (37 °C)
  • JO2,P(NS) = JO2,P(GM)/0.73
  • Fiber wet mass to dry mass ratio = 3.5 (N'Guessan 2004 Mol Cell Biochem)
  • Human vastus lateralis
  • 8 males
  • 46.7 years
  • Sedentary, no regular professinal or recreational physical activity
  • H = 1.78 m
  • M = 84.4 kg
  • BME = 0.28
  • BMI = 26.6 kg·m-2
  • VO2max/M = 30.6 mL·min-1·kg-1 (= VO2peak/M/0.93)
  • Permeabilized muscle fibres; 22 °C; GMP; md; conversions: Gnaiger 2009 Int J Biochem Cell Biol
  • JO2,P(NS) = 68.3 µmol·s-1·kg-1 wet muscle mass (37 °C)
  • JO2,P(GM) = 49.8 µmol·s-1·kg-1 wet muscle mass (37 °C)
  • JO2,P(NS) = JO2,P(GM)/0.73
  • Fiber wet mass to dry mass ratio = 3.5 (N'Guessan 2004 Mol Cell Biochem)

References: BME and VO2max

» VO2max
 Reference
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Boushel 2007 DiabetologiaBoushel RC, Gnaiger E, Schjerling P, Skovbro M, Kraunsoee R, Dela F (2007) Patients with Type 2 diabetes have normal mitochondrial function in skeletal muscle. Diabetologia 50:790-6.
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Garnier 2005 FASEB JGarnier A, Fortin D, Zoll J, N'Guessan B, Mettauer B, Lampert E, Veksler V, Ventura-Clapier R (2005) Coordinated changes in mitochondrial function and biogenesis in healthy and diseased human skeletal muscle. FASEB J 19:43-52.
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Gnaiger 2019 MiP2019
Erich Gnaiger
OXPHOS capacity in human muscle tissue and body mass excess – the MitoEAGLE mission towards an integrative database (Version 6; 2020-01-12).
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Mettauer 2001 J Am Coll CardiolMettauer B, Zoll J, Sanchez H, Lampert E, Ribera F, Veksler V, Bigard X, Mateo P, Epailly E, Lonsdorfer J, Ventura-Clapier R (2001) Oxidative capacity of skeletal muscle in heart failure patients versus sedentary or active control subjects. J Am Coll Cardiol 38:947-54.
Mogensen 2006 J PhysiolMogensen M, Bagger M, Pedersen PK, Fernström M, Sahlin K (2006) Cycling efficiency in humans is related to low UCP3 content and to type I fibres but not to mitochondrial efficiency. J Physiol 571:669-81.
N'Guessan 2004 Mol Cell BiochemN'Guessan B, Zoll J, Ribera F, Ponsot E, Lampert E, Ventura-Clapier R, Veksler V, Mettauer B (2004) Evaluation of quantitative and qualitative aspects of mitochondrial function in human skeletal and cardiac muscles. Mol Cell Biochem 256-257:267-80.
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Rasmussen 2001 Am J Physiol Endocrinol MetabRasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J (2001) Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab 280:E301-7.
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Zoll 2002 J PhysiolZoll J, Sanchez H, N'Guessan B, Ribera F, Lampert E, Bigard X, Surrurier B, Fortin D, Geny B, Veksler V, Ventura-Clapier R, Mettauer B (2002) Physical activity changes the regulation of mitochondrial respiration in human skeletal muscle. J Physiol 543:191-200.


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 


Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS  Pathway: N 


MitoEAGLE BME, BMI, VO2max, BME 

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