Avila-Rojas 2020 Food Chem Toxicol
|Avila-Rojas SH, Aparicio-Trejo OE, Briones-Herrera A, Medina-Campos ON, Reyes-Fermín LM, Martínez-Klimova E, León-Contreras JC, Hernández-Pando R, Tapia E, Pedraza-Chaverri J (2020) Alterations in mitochondrial homeostasis in a potassium dichromate model of acute kidney injury and their mitigation by curcumin. Food Chem Toxicol 145:111774.|
Avila-Rojas Sabino Hazael, Aparicio-Trejo Omar Emiliano, Briones-Herrera Alfredo, Medina-Campos Omar Noel, Reyes-Fermin Laura Maria, Martinez-Klimova Elena, Leon-Contreras Juan Carlos, Hernandez-Pando Rogelio, Tapia Edilia, Pedraza-Chaverri Jose (2020) Food Chem Toxicol
Abstract: Curcumin has protective effects in several acute kidney injury models, including that induced by potassium dichromate (K2Cr2O7). The protective effect of curcumin in this experimental model has been associated to the preservation of mitochondrial bioenergetics. This study is aimed at evaluating whether or not curcumin's protective effect in mitochondrial bioenergetics is related to the modulation of mitochondrial dynamics and biogenesis. Wistar rats were treated with a single subcutaneous dose of K2Cr2O7 (12.5 mg/kg) or received curcumin (400 mg/kg/day) by oral gavage 10 days before and one day after the K2Cr2O7 injection. K2Cr2O7 induced kidney dysfunction and increased mitochondrial hydrogen peroxide production, while decreasing the respiration directly attributable to oxidative phosphorylation and mitochondrial membrane potential. In mitochondria, K2Cr2O7 increased fission and reduced fusion. Structural analysis of mitochondria in the proximal tubular cells corroborated their fragmentation and loss of crests' integrity. Regarding mitochondrial biogenesis, K2Cr2O7 decreased peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) levels. Conversely, curcumin treatment mitigated the aforementioned alterations and increased the expression of the mitochondrial transcription factor A (TFAM). Taken together, our results suggest that curcumin can protect against renal injury by modulating mitochondrial homeostasis, mitigating alterations in bioenergetics and dynamics, possibly by stimulating mitochondrial biogenesis.
• Keywords: Curcumin, Mitochondrial biogenesis, Mitochondrial dynamics, Mitochondrial homeostasis, Nephrotoxicity, Potassium dichromate • Bioblast editor: Plangger M • O2k-Network Lab: MX Mexico City Pedraza Chaverri J
Labels: MiParea: Respiration, mt-Structure;fission;fusion, Pharmacology;toxicology
Organism: Rat Tissue;cell: Kidney Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k, O2k-Fluorometer