OROBOROS INSTRUMENTS

Bioblast Agenda - 2024 week 42

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 42

Oct-14 Mo

Forward or reverse?

If mitochondria are incubated with succinate or glycerolphosphate without rotenone, i.e. in the LEAK state (high mitochondrial membrane potential), electrons can flow from CII to CI instead of the Q-junction and stimulate ROS production.
- »Reverse electron flow from CII to CI«; »SUIT-026«
- communicated by »Schmitt Sabine« and »Willis Jaime R«

Oct-15 Tu

Oct-16 We

Oct-17 Th

"Any moisture on the mesh increases the risk of air bubbles being trapped below the Sample Holder after closing the O2k-chamber."

- »MiPNet27.07 Sample Holder«
- communicated by »Leo Elettra« and »Timon-Gomez Alba«

Oct-18 Fr

Next Week

2024 Week 43

Oct-21 Mo

BIOCEV days 2024 Prague CZ 2024 Oct 21-23

Oct-22 Tu

How is data from the mark statistics window in DatLab 8 exported in the correct format for analysis using the O2 analysis template?

In the mark statistics window (F2), check the option "Export for DL analysis template" and select "O2 analysis template" in the drop-down menu. Copy to the clipboard and paste your data in to the corresponding Excel sheet.
- »Mark statistics - DatLab«
- communicated by »Grings M« and »Willis Jaime R«

Oct-23 We

13:50 Erich Gnaiger presenting at BIOCEV days 2024 Prague CZ - Unlocking cell metabolism: Exploring the power of high-resolution respirometry with the NextGen-O2k

Oct-24 Th

HMTD symposium Prague CZ 2024 Oct 24-25

Oct-25 Fr

14th Mitochondrial Disease Conference 2024 Padova IT 2024 Oct 25-27

In focus

A look back at EBEC2024.

EBEC2024
2024 August 26 – 31 – Innsbruck, Austria

Read the abstracts

»BEC EBEC2024 Late-breaking abstracts

»BBA EBEC2024 Abstract Book

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Oroboros of the month

In the artwork "First Fruits of the Earth Offered to Saturn" at the Palazzo Vecchio, Saturn (Cronus in Greek mythology) is holding the Ouroboros in the hand, symbolizing the eternal cycle of renewal and the interconnectedness of beginning and end.

» Oroboros pictures of the month

Bioblast: About

Bioblast is an open access platform, including MitoPedia as a glossary and index for mitochondrial and chloroplast physiology, hosting MitoGlobal, and supporting the journal Bioenergetics Communications and the Oroboros Ecosystem.
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2024 Week 44

Oct-28 Mo

Oct-29 Tu

Oct-30 We

Oct-31 Th

Get a more in-depth look into mitochondrial function with fluorometry. The O2k-Fluo Smart-Module enables the assessment of ROS production (H₂O₂ flux; Amplex UltraRed), membrane potential (Safranin, TMRM, Rhodamine 123), calcium uptake (CaG), and ATP production (MgG)

- »MiPNet28.09 O2k-Fluo Smart-Module manual« »Fluorometry«
- communicated by »Willis Jaime R« and »Schmitt Sabine«

Nov-01 Fr

2024 Week 45

Nov-04 Mo

Nov-05 Tu

Nov-06 We

Nov-07 Th

"Complex I and complex III defects deeply impact on bioenergetic parameters in brain but not liver mitochondria."

Balmaceda V, Komlodi T, Szibor M, Gnaiger E, Moore AL, Fernandez-Vizarra E, Viscomi C (2024) The striking differences in the bioenergetics of brain and liver mitochondria are enhanced in mitochondrial disease. »Bioblast link«

from the O2k-Network »UK Brighton Moore AL«, »DE Jena Szibor M«, »IT Padova Viscomi C«, and »AT Innsbruck Oroboros«

Nov-08 Fr

Nov-09

Nov-10

Oroboros O2k-Workshop IOC168 Dharwad IN

2024 Week 46

Nov-11 Mo

SMRM2024 Dharwad IN 2024 Nov 11-12

Nov-12 Tu

Nov-13 We

Nov-14 Th

"The instrumental background tests must be carried out with the Q-Stopper and reference electrode in place. The values obtained with the standard stoppers for respirometry cannot be used for Q-Sensor experiments."

- »MiPNet24.12 NextGen-O2k: Q-Module«
- communicated by »Grings Mateus« and »Schmitt Sabine«

Nov-15 Fr

2024 Week 47

Nov-18 Mo

Can Amplex® UltraRed be used to measure ROS production in all tissue types and preparations?

Not all tissue types and preparations can be used. Liver homogenate cannot be used with the Amplex® UltraRed assay due to the cytosolic components of liver tissue which interfere with the measurement. Isolating liver mitochondria overcomes this drawback.
- »Amplex UltraRed« »Tissue homogenate«
- communicated by »Willis Jaime R« and »Leo Elettra«

Nov-19 Tu

Nov-20 We

Nov-21 Th

Nov-22 Fr

2024 Week 48

Nov-25 Mo

Nov-26 Tu

Nov-27 We

Nov-28 Th

Nov-29 Fr

2024 Week 49

Dec-02 Mo

Dec-03 Tu

Dec-04 We

Dec-05 Th

Dec-06 Fr

2024 Week 50

Dec-09 Mo

Metal chelation decreases the high background flux in the Amplex® UltraRed assay

DTPA (Diethylenetriaminepentaacetic acid) is a metal chelator that can be incorporated into the Amplex® UltraRed assay to decrease the high background fluxes associated with iron contamination.
- »DTPA«; »Amplex UltraRed«
- communicated by »Willis Jaime R« and »Grings Mateus«

Dec-10 Tu

Dec-11 We

Dec-12 Th

Dec-13 Fr

2024 Week 51

Dec-16 Mo

Dec-17 Tu

Dec-18 We

Dec-19 Th

Dec-20 Fr

2024 Week 52

Dec-23 Mo

How can titration artifacts in the flux plot be hidden to have a more clear figure of the trace?

Select the corresponding plot in one of the graphs, press Ctrl+Shift+left click at the chosen starting time point and drag the cursor over the section to be hidden.
- »Hide/Show points - DatLab«
- communicated by »Grings Mateus« and »Baglivo Eleonora«

Dec-24 Tu

Dec-25 We

Dec-26 Th

Dec-27 Fr

Last week and before

» Oroboros Ecosystem Agenda 2024 Archive

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