OROBOROS INSTRUMENTS

Bioblast Agenda - 2023 week 50

Oroboros declares solidarity against the Russian aggression-war in Ukraine.

2023 Week 50

Dec- 11 Mo - International Mountain Day

Mountain slopes can be difficult, but O₂ slopes can be smooth with DatLab

The default setting of Slope smoothing is 40 data points, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.
- »Smoothing in Flux/Slope«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

Dec- 12 Tu

From preprint to Gold Open Access publication in J Biol Chem

Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. »Bioblast link«

Dec- 13 We

Dec- 14 Th

"HCR [High capacity runner rats] showed a consistent and robust trend of higher maximal oxygen consumption compared to LCR [low capacity runner rats], especially for long chain FAs [fatty acids]."

Fleischman JY, Van den Bergh F, Collins NL, Bowers M, Beard DA, Burant CF (2023) Higher mitochondrial oxidative capacity is the primary molecular differentiator in muscle of rats with high and low intrinsic cardiorespiratory fitness. »Bioblast link«

from the O2k-Network »US MI Ann Arbor Beard DA«

Dec- 15 Fr

Next Week

2023 Week 51

Dec- 18 Mo

»SBC 2023 Goa IN« Dec 18-20

Why add azide after ascorbate and TMPD when measuring Complex IV activity?

The O₂ flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O₂ flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement.
- »Complex IV«, »Chemical background «
- communicated by »Grings Mateus« and »Timon-Gomez Alba«

Dec- 19 Tu

Dec- 20 We

"SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."

Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. »Bioblast link«

from the O2k-Network »US FL Orlando Goodpaster BH« and »IE Dublin O Gorman D«

Dec- 21 Th

Dec- 22 Fr

In focus

Join Mito & Chlora for discussing latest trends on all topics from bioenergetics to mitochondrial diseases.

EBEC2024

Date: 2024 August 26 – 31

Location: Innsbruck, Austria

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Oroboros of the month

An Ouroboros tattoo observed during an O2k-Workshop, made by the tattoo artist @vicky.ink

Artist: @vicky.ink (IG channel)

» Oroboros pictures of the month

Bioblast: About

Bioblast is an open access platform, including MitoPedia as a glossary and index for mitochondrial and chloroplast physiology, hosting MitoGlobal, and supporting the journal Bioenergetics Communications and the Oroboros Ecosystem.
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2023 Week 52

Dec- 25 Mo

Dec- 26 Tu

Dec- 27 We

Do you observe a high signal at zero oxygen?

If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electrical component is generating the out-of-scale signal.
- »Leak current test«
- communicated by »Baglivo Eleonora« and »Grings Mateus«

Dec- 28 Th

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Last week and before

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