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Next Bioblast Agenda 2025
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2025 Week 5
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"Magnesium Green has no significant effect on respiration of [equine skeletal muscle] isolated mitochondria, whereas tetramethylrhodamine inhibits phosphorylating respiration and increases LEAK respiration."
- Davis MS, Barrett MR, Bayly WM, Bolinger A (2024) Effect of selected fluorophores on equine skeletal muscle mitochondrial respiration. »Bioblast link«
- from the O2k-Network »US OK Stillwater Davis MS«
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Is it possible to measure ATP production by measuring only respiration?
The difference in the O2 flux between ROUTINE and LEAK respiration (living cells, before and after addition of oligomycin), or between OXPHOS and LEAK respiration (mitochondrial preparations) is not a direct measurement of ATP production. It represents the respiratory activity available for phosphorylation of ADP to ATP.
- »Magnesium Green«
- communicated by »Grings Mateus« and »Cardoso Luiza«
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2025 Week 6
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Is it sufficient to perform only one H2O2 calibration in the Amplex® UltraRed assay?
No, multiple H2O2 calibrations should be performed because H2O2 sensitivity might change due to (1) experimental duration and accumulating UltroxRed, (2) changes in optical properties due to titrations, and (3) the radical scavenging capacity of the sample.
- »Amplex UltraRed«, »Komlodi 2021 BEC«
- communicated by »Timon-Gomez Alba« and »Schmitt Sabine«
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Mitochondrial ROS production in microvascular endothelial cells was shown to be reduced by aerobic exercise training in recent (≤ 5 years) but not in late (≥ 10 years) postmenopausal females.
- Noerregaard LB, Hansen CC, Wickham KA, Moeller S, Olsen K, Ehlers T, Bangsbo J, Hellsten Y (2024) Exercise training alters skeletal muscle microvascular endothelial cell properties in recent postmenopausal females. »Bioblast link«
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2025 Week 7
2025 Week 8
2025 Week 9
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