OROBOROS INSTRUMENTS

Bioblast Agenda - 2024 week 40

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 40

Sep-30 Mo

Oroboros O2k-Workshop IOC167 Schroecken AT Sep 30-Oct 05

Proton pump or hydrogen ion pump?

Although used synonymously in chemistry, in particle physics a proton p⁺ is a positively charged subatomic particle and a hydrogen ion H⁺ is a positively charged ion (cation). CI, CIII, CIV, and ATP synthase are mitochondrial hydrogen ion pumps.
- »Hydrogen ion pump«; »Hydrogen ion«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«

Oct-01 Tu

Oroboros O2k-Workshop IOC167 Schroecken AT Sep 30-Oct 05

Oct-02 We

Oroboros O2k-Workshop IOC167 Schroecken AT Sep 30-Oct 05

Oct-03 Th

Oroboros O2k-Workshop IOC167 Schroecken AT Sep 30-Oct 05

"It is important to have the correct O2k-chamber volume entered for calculations of oxygen flux. Entries can be edited at any time during real-time or post-experiment analysis."

- »MiPNet24.14 O2k-sV-Module manual«
- communicated by »Timon-Gomez Alba« and »Willis Jaime R«

Oct-04 Fr

Oroboros O2k-Workshop IOC167 Schroecken AT Sep 30-Oct 05

Oct-05 Sa

Oroboros O2k-Workshop IOC167 Schroecken AT Sep 30-Oct 05

Next Week

2024 Week 41

Oct-07 Mo

Do you know all keyboard shortcuts for the DatLab software?

DatLab provides several shortcuts for quick access to functions and settings without the need for a mouse.
- »Keyboard shortcuts - DatLab«
- communicated by »Baglivo Eleonora« and »Willis Jaime R«

Oct-08 Tu

Oct-09 We

Oct-10 Th

Oct-11 Fr

In focus

A look back at EBEC2024.

EBEC2024
2024 August 26 – 31 – Innsbruck, Austria

Read the abstracts

»BEC EBEC2024 Late-breaking abstracts

»BBA EBEC2024 Abstract Book

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Oroboros of the month

In the artwork "First Fruits of the Earth Offered to Saturn" at the Palazzo Vecchio, Saturn (Cronus in Greek mythology) is holding the Ouroboros in the hand, symbolizing the eternal cycle of renewal and the interconnectedness of beginning and end.

» Oroboros pictures of the month

Bioblast: About

Bioblast is an open access platform, including MitoPedia as a glossary and index for mitochondrial and chloroplast physiology, hosting MitoGlobal, and supporting the journal Bioenergetics Communications and the Oroboros Ecosystem.
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2024 Week 42

Oct-14 Mo

Forward or reverse?

If mitochondria are incubated with succinate or glycerolphosphate without rotenone, i.e. in the LEAK state (high mitochondrial membrane potential), electrons can flow from CII to CI instead of the Q-junction and stimulate ROS production.
- »Reverse electron flow from CII to CI«; »SUIT-026«
- communicated by »Schmitt Sabine« and »Willis Jaime R«

Oct-15 Tu

Oct-16 We

Oct-17 Th

"Any moisture on the mesh increases the risk of air bubbles being trapped below the Sample Holder after closing the O2k-chamber."

- »MiPNet27.07 Sample Holder«
- communicated by »Leo Elettra« and »Timon-Gomez Alba«

Oct-18 Fr

2024 Week 43

Oct-21 Mo

How is data from the mark statistics window in DatLab 8 exported in the correct format for analysis using the O2 analysis template?

In the mark statistics window (F2), check the option "Export for DL analysis template" and select "O2 analysis template" in the drop-down menu. Copy to the clipboard and paste your data in to the corresponding Excel sheet.
- »Mark statistics - DatLab«
- communicated by »Grings M« and »Willis Jaime R«

Oct-22 Tu

Oct-23 We

Oct-24 Th

Oct-25 Fr

14th Mitochondrial Disease Conference 2024 Padova IT 2024 Oct 25-27

2024 Week 44

Oct-28 Mo

Oct-29 Tu

Oct-30 We

Oct-31 Th

Get a more in-depth look into mitochondrial function with fluorometry. The O2k-Fluo Smart-Module enables the assessment of ROS production (H₂O₂ flux; Amplex UltraRed), membrane potential (Safranin, TMRM, Rhodamine 123), calcium uptake (CaG), and ATP production (MgG)

- »MiPNet28.09 O2k-Fluo Smart-Module manual« »Fluorometry«
- communicated by »Willis Jaime R« and »Schmitt Sabine«

Nov-01 Fr

2024 Week 45

Nov-04 Mo

Nov-05 Tu

Nov-06 We

Nov-07 Th

Nov-08 Fr

2024 Week 46

Nov-11 Mo

Nov-12 Tu

Nov-13 We

Nov-14 Th

Nov-15 Fr

2024 Week 47

Nov-18 Mo

Can Amplex® UltraRed be used to measure ROS production in all tissue types and preparations?

Not all tissue types and preparations can be used. Liver homogenate cannot be used with the Amplex® UltraRed assay due to the cytosolic components of liver tissue which interfere with the measurement. Isolating liver mitochondria overcomes this drawback.
- »Amplex UltraRed« »Tissue homogenate«
- communicated by »Willis Jaime R« and »Leo Elettra«

Nov-19 Tu

Nov-20 We

Nov-21 Th

Nov-22 Fr

2024 Week 48

Nov-25 Mo

Nov-26 Tu

Nov-27 We

Nov-28 Th

Nov-29 Fr

2024 Week 49

Dec-02 Mo

Dec-03 Tu

Dec-04 We

Dec-05 Th

Dec-06 Fr

2024 Week 50

Dec-09 Mo

Metal chelation decreases the high background flux in the Amplex® UltraRed assay

DTPA (Diethylenetriaminepentaacetic acid) is a metal chelator that can be incorporated into the Amplex® UltraRed assay to decrease the high background fluxes associated with iron contamination.
- »DTPA«; »Amplex UltraRed«
- communicated by »Willis Jaime R« and »Grings Mateus«

Dec-10 Tu

Dec-11 We

Dec-12 Th

Dec-13 Fr

2024 Week 51

Dec-16 Mo

Dec-17 Tu

Dec-18 We

Dec-19 Th

Dec-20 Fr

2024 Week 52

Dec-23 Mo

Dec-24 Tu

Dec-25 We

Dec-26 Th

Dec-27 Fr

Last week and before

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