OROBOROS INSTRUMENTS

Oroboros Ecosystem Agenda - 2021 week 48

2021 Week 48

Nov-29 Mo

Nov-30 Tu

Targeting mitochondrial translation – a potential therapeutic strategy to suppress glioblastoma stem cell growth

Sighel D, Notarangelo M, Aibara S, Re A, Ricci G, Guida M, Soldano A, Adami V, Ambrosini C, Broso F, Rosatti EF, Longhi S, Buccarelli M, D'Alessandris QG, Giannetti S, Pacioni S, Ricci-Vitiani L, Rorbach J, Pallini R, Roulland S, Amunts A, Mancini I, Modelska A, Quattrone A (2021) Inhibition of mitochondrial translation suppresses glioblastoma stem cell growth. Cell Rep 35:109024. »Bioblast link«

from the O2k-Network »IT Bolzano Pichler I«

Dec-01 We

Dec-02 Th

Coenzyme Q - a mobile redox compound in the electron transfer system

Coenzyme Q is a lipid composed of a benzoquinone ring with an isoprenoid side chain, two methoxy groups and one methyl group being present in all tissues and cells. In humans, CoQ₁₀ occurring in the mitochondrial electron transfer system transfers electrons from dehydrogenases and respiratory complexes downstream to Complex III.
- »Coenzyme Q«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Dec-03 Fr

Oroboros of the month

Oroboros in Copenhagen

Before departing for the Greenland 2004 expedition, Erich Gnaiger strolled through the Kierkegaard graveyard in Copenhagen, observing a suprising variety of Ouroboros emblems - symbols of transformation and eternal recycling (2004-02-22; photo by Erich Gnaiger).

Oroboros pictures of the month page

In focus

Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003«

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Oroboros Agenda In Focus

Funding acknowledgments

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2021 Week 49

Dec-06 Mo

Dec-07 Tu

What is ROUTINE respiration?

ROUTINE respiration is the respiratory activity of living (non-permeabilized) cells in the physiological coupling state, which is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation.
- »Gnaiger 2020 BEC MitoPathways - Chapter 2.4«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Dec-08 We

The central role of the Q-junction in mitochondria

The Q-junction is a converging point in the electron transfer system. Electrons flow from different dehydrogenases and respiratory Complexes into the Q-junction and then downstream to Complex III.
- »Q-junction«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Dec-09 Th

How do you measure ROUTINE respiration?

ROUTINE respiration (R) of living cells is measured before permeabilization of the cell membrane either in cell culture medium or any other respiration media (e.g. MiR05) in the presence or absence of exogenous substrates. Allow R to stabilize for 15-20 min.
- »ROUTINE respiration« and »Doerrier 2018 Methods Mol Biol«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Dec-10 Fr

2021 Week 50

Dec-13 Mo

Why use a short-chain coenzyme Q mimetic for measurement of the Q-redox state using the Q-Module?

The naturally occurring long-isoprenoid chain coenzyme Q (e.g. CoQ₁₀ or CoQ₉) is trapped within membrane boundaries. Therefore, a short-chain, membrane-permeable CoQ mimetic, CoQ₂ is used as a probe which reacts with the mitochondrial respiratory Complexes at their quinone-binding sites and with the working electrode of the Q-sensor.
- »Coenzmye Q₂« and »Q-Module«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

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2021 Week 51

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2021 Week 52

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2022

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»10th Conference of the International Coenzyme Q10 Association 2022 Hamburg DE« May 12-15

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2022 Week 46

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2022 Week 47

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2022 Week 48

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2022 Week 49

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2022 Week 50

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2022 Week 51

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2022 Week 52

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Last week and before

» Oroboros Ecosystem Agenda 2021 Archive

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OROBOROS INSTRUMENTS

2022

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