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Bioblast Agenda - 2024 week 3

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2025 Week 3

Jan-13 Mo

The phosphorylation system: More than just ATP synthase

The phosphorylation system (phosphorylation pathway) is the functional unit that utilizes the protonmotive force (pmF) to phosphorylate ADP to ATP. The system consists of adenine nucleotide translocase (ANT), phosphate carrier, and ATP synthase. These components ensure the supply of ADP and inorganic phosphate for utilization by ATP synthase.
- »Phosphorylation pathway «
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

Jan-14 Tu

Jan-15 We

“In more than a year of performing these protocols, I have not achieved results like the ones from this last experiment. I am very grateful for your assistance thus far.”

Luís Felipe G Valdivia, from the O2k-Network »BR Sao Paulo Festuccia W«, after receiving tips through Oroboros customer support
- »Oroboros Feedback«

Jan-16 Th

Jan-17 Fr

Next Week

2025 Week 4

Jan-20 Mo

Beyond O₂ consumption — measuring ATP dynamics with Magnesium Green (MgG)

MgG fluoresces when bound to Mg²⁺. By taking advantage of the different binding affinities of ADP and ATP for Mg²⁺, we can follow ATP production across a variety of mitochondrial preparations. As ATP is produced, more Mg²⁺ is bound, and the MgG fluorescence decreases.
- »Magnesium Green« »Cardoso 2021 BEC MgG«
- communicated by »Willis Jaime R« and »Cardoso Luiza«

Jan-21 Tu

Jan-22 We

Jan-23 Th

"Assessment of ATP dynamics in real-time showed that the mitochondrial capacity to produce ATP was diminished at elevated temperatures as ATP hydrolysis rates increased."

Willis JR, Hickey AJR, Devaux JBL (2021) Thermally tolerant intertidal triplefin fish (Tripterygiidae) sustain ATP dynamics better than subtidal species under acute heat stress. Sci Rep 11:11074. »Bioblast link«

from the O2k-Network »NZ Auckland Hickey AJ«

Jan-24 Fr

In focus

2025 Oroboros Workshops:
Oroboros Workshop on high-resolution respirometry
2025 Jun 23 - 28
Schroecken, Austria
Oroboros Workshop on high-resolution respirometry
2025 Sep 29 - Oct 04
Schroecken, Austria

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Oroboros of the month

In the artwork "First Fruits of the Earth Offered to Saturn" at the Palazzo Vecchio, Saturn (Cronus in Greek mythology) is holding the Ouroboros in the hand, symbolizing the eternal cycle of renewal and the interconnectedness of beginning and end.

» Oroboros pictures of the month

Bioblast: About

Bioblast is an open access platform, including MitoPedia as a glossary and index for mitochondrial and chloroplast physiology, hosting MitoGlobal, and supporting the journal Bioenergetics Communications and the Oroboros Ecosystem.
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2025 Week 5

Jan-27 Mo

"Magnesium Green has no significant effect on respiration of [equine skeletal muscle] isolated mitochondria, whereas tetramethylrhodamine inhibits phosphorylating respiration and increases LEAK respiration."

Davis MS, Barrett MR, Bayly WM, Bolinger A (2024) Effect of selected fluorophores on equine skeletal muscle mitochondrial respiration. »Bioblast link«

from the O2k-Network »US OK Stillwater Davis MS«

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2025 Week 6

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2025 Week 7

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2025 Week 8

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2025 Week 9

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Last week and before

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