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Oroboros Ecosystem Agenda - 2020 week 48
2020 Week 48
 
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Cell culture medium and dithionite titrations – a bad combination for an instrumental O2 background test?
Cell culture media are not ideal for instrumental O2 background test when using dithionite titrations because side reactions affecting the O2 concentration and thus interfering with the test. Instead of cell culture media, use MiR05, MiR06 or a strongly buffered alkaline phosphate solution.
-»MiPNet14.06 Instrumental O2 background«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«
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- ISCaM 2020 Virtual Event
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Eight-week high-fat feeding increases mitochondrial ROS production in white adipocytes but mitochondrial respiration remains unaffected.
- from the O2k-Network »CA Guelph Holloway GP«
- Politis-Barber V, Brunetta HS, Paglialunga S, Petrick HL, Holloway GP (2020) Long-term high-fat feeding exacerbates short-term increases in adipose mitochondrial reactive oxygen species, without impairing mitochondrial respiration. Am J Physiol Endocrinol Metab 319:E376-87. »Bioblast link«
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HSP60 knockdown and its effect on mitochondria’s role in pancreatic ductal adenocarcinoma
- Zhou C, Sun H, Zheng C, Gao J, Fu Q, Hu N, Shao X, Zhou Y, Xiong J, Nie K, Zhou H, Shen L, Fang H, Lyu J (2018) Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth. Cell Death Dis 9:161. »Bioblast link«
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Oroboros of the month
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- Full Circle
- This image was created for the cover of an issue of "Angewandte Chemie" that featured an article on geometric and electronic connections between different types of pericyclic reactions. The background is a page from Woodward and Hoffmann's classic Conservation of Orbital Symmetry.
- The circle is an orobouros - a mythical serpent eating its own tail - that was created by Prof. Tantillo in response to a suggestion by Roald Hoffmann; and emphasizes both the cyclic nature of pericyclic transition structures as well as the fact that the article accompanying this illustration marked Roald's return to the topic of simple pericyclic reactions of hydrocarbons, nearly three decades after he first described many of their key electronic features.
- (Dean J Tantillo -link)
- Oroboros pictures of the month page
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In focus
- 0.5-mL and 2-mL O2k-chambers - flexibility in high-resolution respirometry
- featured MitoPedia page: »Cell count and normalization in HRR«
Last update November 27, 2020
 Bioblast alert 2020(13)
| Some terms in “Mitochondrial physiology” Bioenergetics Communications 2020.1 (States and rates) did not look familiar ― what is the elementary unit [x]?
Fundamental answers, a critical discussion on the SI unit for count, and the meaning of Euclid’s unit and Euclid’s number are presented in an unusual ‘canonical review’:
- Gnaiger Erich (2020) Canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. MitoFit Preprint Arch 2020.4 doi:10.26124/mitofit:200004. - »Bioblast link«
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Stay alert
Funding acknowledgments
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Bioblast alert 2020
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