OROBOROS INSTRUMENTS

»Oroboros Ecosystem«

Oroboros Ecosystem Agenda - 2020 week 40

2020 Week 40

Sep-28 Mo - ESCI 2020 Virtual Event

Are whitish deposits forming on the glass wall of the O2k-Chamber?

These deposits might be an accumulation of proteins and subsequent embedding of other biological material or small glass splinters, and a sign that the O2k-Chamber requires cleaning.
For further details on how to clean the O2k-Chamber properly, see:
- »Cleaning of the O2k-Chamber«
- communicated by »Komlodi Timea«, »Sabine Schmitt«, and »Lisa Tindle-Solomon«

Sep-29 Tu - ESCI 2020 Virtual Event

World Heart Day

Succinate dehydrogenase inhibition protects myocardium from ischemia reperfusion injury but efficacy may be influenced by diabetes

from the O2k-Network »DK Aarhus Boetker HE« and »DK Copenhagen Larsen S«

Jespersen NR, Hjortbak MV, Lassen TR, Støttrup NB, Johnsen J, Tonnesen PT, Larsen S, Kimose HH, Bøtker HE (2020) Cardioprotective effect of succinate dehydrogenase inhibition in rat hearts and human myocardium with and without diabetes mellitus. Sci Rep 10:10344. »Bioblast link«

As a Horizon 2020 beneficiary, Oroboros signed the Manifesto for EU COVID-19 Research, showing our support for the accessibility of research results in the fight against COVID-19.

Sep-30 We - ESCI 2020 Virtual Event

Early registration deadline - »ESSR 2020 Innsbruck AT«

Oct-01 Th

Abstract submission deadline for: Integrative Physiology of Exercise 2020 Virtual Event

Oct-02 Fr

Oroboros of the month

The Ouroboros symbol

In this personal Symbology by Adam Sass, he writes

about his discovery of the ouroboros symbol and its meaning.

Story available here. (Sass A, 2003)

Oroboros pictures of the month page

Yearly calendar - last update September 29, 2020

In focus

Bioblast alert 2020(13)

Some terms in “Mitochondrial physiology” Bioenergetics Communications 2020.1 (States and rates) did not look familiar ― what is the elementary unit [x]?

Fundamental answers, a critical discussion on the SI unit for count, and the meaning of Euclid’s unit and Euclid’s number are presented in an unusual ‘canonical review’:

Gnaiger Erich (2020) Canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. MitoFit Preprint Arch 2020.4 doi:10.26124/mitofit:200004. - »Bioblast link«

Join the discussion on the MitoEAGLE Consortium Communication: “Mitochondrial physiology” Bioenergetics Communications 2020.1

» On a Comment in Nature Metabolism «

Stay alert

Bioblast alert 2020

MitoPedia alert - Currently Asked Questions

O2k-Publications alert

O2k-Open Support alert - Currently Asked Questions

Oroboros Agenda highlights

Funding acknowledgments

Next Oroboros Ecosystem Agenda 2020

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2020 Week 41

Oct-05 Mo

Oct-06 Tu - ÖGMBT Life Science Tuesdays

Fasting promotes functional changes in liver mitochondria: Is it time to reconsider using fasted animals for liver mitochondria isolation?

from the O2k-Network »BR Sao Paulo Kowaltowski AJ«

Menezes-Filho SL, Amigo I, Luévano-Martínez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35. »Bioblast link«

Oct-07 We

Background H₂O₂ calibration: what is it and why perform it when using Amplex UltraRed assay?

Background H₂O₂ calibration refers to a set of H₂O₂ titrations conducted after addition of respiration medium, DTPA, HRP, SOD and Amplex UltraRed to the O2k-chamber but before sample addition. This step is required 1) for the calculation of the theoretical chemical background fluorescence in our Excel analysis template, and 2) for the calculation of the sensitivity of the AmR assay, which serves as a quality control of the assay.
- »Background H₂O₂ calibration«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Oct-09 Fr

»Long Night of Research 2020 Innsbruck AT«

2020 Week 42

Oct-12 Mo http://www.metabesity2020.com/

If using the Amplex UltraRed assay, when should the background H₂O₂ calibration be performed?

Background H₂O₂ calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H₂O₂ is prepared, or if a different respiration medium is used. As the H₂O₂ stock solution needs to be freshly prepared, it is recommended to perform a background H₂O₂ calibration before each experiment and at a minimum once per experimental day.
- »How to find the background H₂O₂ calibration protocol in DatLab « select “AmR calibration” for the background H₂O₂ calibration protocol
- communicated by »Komlodi Timea« and »Schmitt Sabine«