MitoPedia alert

MitoPedia alert

MitoPedia alert - 2021

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

Nov 2021

Nov-24 We

Mitochondrial H₂O₂ production was discovered 50 years ago by the group of Britton Chance

Check out how to measure H₂O₂ production using the Amplex UltraRed assay in combination with high-resolution respirometry.
- »Loschen 1971 FEBS Letters« and »Amplex UltraRed« and »O2k-Fluo LED2-Module« »O2k-Fluo Smart-Module«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Jul 2021

Jul-07 We

TPP⁺ for potentiometric detection of mt-membrane potential (mtMP) or fluorometry with safranin?

Safranin enables measurement of the relationship between fluorescence intensity and mtMP, and if linear, the absolute mtMP can be calculated. TPP⁺ enables calibration for measurement of mtMP.
- »TPP⁺ vs safranin«

Jun 2021

Jun-24 Th

Do you know how to select the correct fluorescence sensors and filter set?

Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) enables measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«

May 2021

May-24 Mo

Immediately after switching off the light, a sharp peak of light-enhanced dark respiration is observed in algae.

- »Light-enhanced dark respiration«

May-11 Tu

Measuring succinate pathway (S-pathway) in living cells

Using MitoKit-CII – cell permeable succinate and malonate, the S-pathway can be measured
- »SUIT-003 O2 ce D060«
- communicated by »Cecatto Cristiane« and »Komlodi Timea«

Apr 2021

Mar 2021

Mar-16 Tu

CI inhibitors like rotenone inhibit fatty acid oxidation (FAO)-linked respiration

Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD⁺ blocks FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«

Mar-11 Th

Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?

A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3«
- » Posted by: »Komlodi Timea« and »Cecatto Cristiane«

Mar-2 Tu

Measuring fatty acid oxidation (FAO)-linked respiration requires a low concentration of malate

In the absence of malate, accumulation of Acetyl-CoA inhibits FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cecatto Cristiane« and »Cardoso Luiza«

Jan 2021

Jan-21 Th

Measuring ATP production with Magnesium Green – which sample preparations work?

Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
- communicated by »Cardoso Luiza« and »Komlodi Timea«