MitoPedia alert

MitoPedia alert

MitoPedia alert - 2020

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

Aug 2020

Aug-06 Th

OXPHOS-coupling efficiency (1-L/P) describes the coupling of the mitochondrial electron transfer system. This coupling control factor ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (L=P). It is suggested to use OXPHOS-coupling efficiency instead of the respiratory acceptor control ratio (RCR) to evaluate mitochondrial coupling.

- »OXPHOS-coupling efficiency«

Previous MitoPedia Alert 2020

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Jul 2020

Jul-30 Th

Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:

- »Chlororespiration«

Jul-24 Fr

Do you know how to assess the integrity of the mitochondrial outer membrane in mitochondrial preparations?

It is possible to do so using exogenously added cytochrome c:
- »Cytochrome c control factor«

Jul-16 Th

Do you know how to select the proper fluorescence sensors and filter set?

Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«

Jul-09 Th

Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?

- »Setting the oxygen concentration«

Jul-02 Th

Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:

- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity. Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«

Jun 2020

Jun-25 Th

Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?

- » Dithionite«

Jun-18 Th

Did you know the Crabtree effect can affect respiration in living cells?

SUIT-003 is designed to analyze this important mechanism of respiratory control.
- »SUIT-003 O2 ce D037« and »SUIT-003 O2 ce D038«

Jun-11 Th

Did you know that some chemicals are incompatible with the Amplex UltraRed assay?

- »Incompatible substrates«

Jun-08 Mo

Celebrating World Oceans Day

Would you like to measure the O₂ flux that occurs during photosynthesis using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 NextGen-O2k project.
- »PhotoBiology«

Jun-04 Th

Does your sample display a preference for glutamate & malate over pyruvate & malate to support NADH-linked respiration?

DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates.
-»SUIT-008« and »SUIT-014«

May 2020

May-28 Th

Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas?

- »Nitrogen injection«

May-21 Th

As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O₂ consumption and production.

-»PhotoBiology«

May-14 Th

How can I measure ATP production with the O2k?

Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green
- »Magnesium Green«

May-07 Th

Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it?

Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - »malate-anaplerotic pathway control state«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«

Apr 2020

Apr-24 Fr

ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.

- »Hatefi 1962 J Biol Chem-XLII«

Apr-21 Tu

Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.

- »MiPNet17.04 CitrateSynthase«

Apr-13 Mo

Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?

DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
- »SUIT-024«

Apr-08 We

The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.

DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
- »SUIT-010«

Apr-02 Th

Do you want to search the best SUIT protocol for your research questions?

- Use the SUITbrowser: »SUITbrowser«

Mar 2020

Mar-16 Mo

The standardized coupling control protocol to explore the bioenergetics of living cells

DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions.
- »SUIT-003«

Mar-11 We

Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.

- »Blebbistatin«