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Oxygen solubility factor

From Bioblast


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Oxygen solubility factor

Description

The oxygen solubility factor of the incubation medium, FM, expresses the effect of the salt concentration on oxygen solubility relative to pure water. In mitochondrial respiration medium MiR05, MiR05-Kit and MiR06, FM is 0.92 (determined at 30 and 37 ยฐC) and in culture media is 0.89 (at 37 ยฐC). FM varies depending on the temperature and composition of the medium. To determine the FM based on the oxygen concentration, specific methods and equipment are needed (see references Rasmussen HN, Rasmussen UF 2003 in MiPNet06.03). For other media, FM may be estimated using Table 4 in MiPNet06.03. For this purpose KCl based media can be described as "seawater" of varying salinity. The original data on sucrose and KCl-media (Reynafarje et al 1985), however, have been critizesed as artefacts and the FM of 0.92 is suggested in the temperature range of 10 ยฐC to 40 ยฐC as for MiR05.

Abbreviation: FM

Reference: MiPNet06.03 POS-calibration-SOP

Keywords


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Concentration
ยป Volume
ยป Activity
ยป Concentration
ยป Density
ยป Mole
ยป Molar mass
Pressure
ยป Barometric pressure
ยป Chemiosmotic pressure
ยป Gas pressure
ยป Osmotic pressure
ยป Oxygen pressure
ยป pascal
ยป Pressure
Solubility = concentration/pressure
ยป Solubility
ยป Solubility factor
ยป Oxygen solubility
General
ยป Boltzmann constant
ยป Energy
ยป Force
ยป Gas constant
ยป Work
Related keyword lists
ยป Keywords: Oxygen signal
ยป Keywords: Normalization



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cO2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. FM has been determined for MiR05 at 30 ยฐC and 37 ยฐC, and is assumed to be identical at 25 ยฐC, and identical in MiR06.


MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: DatLab 


MitoPedia topics: Media for respirometry 


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HRR: Theory