Oxygen solubility factor

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Oxygen solubility factor

Description

The oxygen solubility factor of the incubation medium, FM, expresses the effect of the salt concentration on oxygen solubility relative to pure water. In mitochondrial respiration medium MiR05, MiR05-Kit and MiR06, FM is 0.92 (determined at 30 and 37 °C) and in culture media is 0.89 (at 37 °C). FM varies depending on the temperature and composition of the medium. To calculate the FM based on the oxygen concentration, specific methods and equipment are needed (see references Rasmussen HN, Rasmussen UF 2003 in MiPNet06.03. For other media, FM may be estimated using Table 4 in MiPNet06.03. For this purpose KCl based media can be described as "seawater" of varying salinity.

Abbreviation: FM

Reference: MiPNet06.03 POS-calibration-SOP

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cO2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. FM has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C, and identical in MiR06.


MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: DatLab 


MitoPedia topics: Media for respirometry 


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