Oroboros Open Support |
- Currently Asked Questions - Β»Oroboros Open Support alertΒ«
Oroboros Open Support
- Oroboros Open Support provides technical help, including Open Access pages that help to optimally use the Oroboros, to apply instrumental Oroboros Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.
- MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
- Oroboros Procedures: explain various applications of the Oroboros with Demo experiments
- Oroboros Manual: topic specific manuals with step by step help
- Oroboros Videosupport: provides valuable assistance, complementary to the Oroboros Manual
- Oroboros Products: is not merely a 'List of products', it presents a guided tour to the Oroboros and integrates the concept of Gentle Science and Open Science.
- Oroboros Open Support provides technical help, including Open Access pages that help to optimally use the Oroboros, to apply instrumental Oroboros Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.
- Oroboros Quality Control
- Many commonly raised issues of Oroboros Open Support are solved in the newest version of DatLab.
- The O2 sensor and Oroboros chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.
- Clean Oroboros chambers and contamination-free incubation medium are required.
- Analysis of DatLab files recorded in instrumental QC tests is essential.
- Biological experiments are not suitable for trouble shooting.
- The O2 sensor and Oroboros chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.
1. O2 sensor test
- The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
- Β» O2 sensor test: QC1: Oxygen sensor test
2. Oroboros chamber test
- If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the Oroboros chamber assembly has to be tested, described in detail:
- Β» Oroboros chamber test: QC2: Instrumental O2 background test
Oroboros Open Support: Installation and startup support session
- Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.
Oroboros Open Support agreement
- The Oroboros experts will be happy to help in using our Oroboros Open Support system.
- Β» Search for defective O2k components
- Β» In case you need further technical support and for your valuable feedback, please contact Oroboros Open Support
- Β» Oroboros Open Support agreement
- Β» O2k repair
The O2k-Network
- The Oroboros Network serves to connect and support: Contact for advice on applications of the Oroboros and high-resolution respirometry - the global network in mitochondrial physiology.
- Β» The O2k-Network Labs: Β» O2k-Network
- Β» Oroboros Laboratory - open for innovation and cooperation.
- Β» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry