O2k-Manual

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O2k-Manual

The O2k-Manual helps beginners and experienced users. Context sensitive help is provided in the DatLab software.

» Polarographic oxygen sensors: calibration, accuracy and quality control SOP » MiPNet06.03 POS-calibration-SOP

» Instrumental O₂ background correction, accuracy of oxygen flux and SOP » MiPNet14.06 Instrumental O2 background

O2k-FluoRespirometer manual: Series H - I and DatLab 7

Chapter	View	Section	Last update
MiPNet24.14 O2k-sV-Module manual	»Versions	O2k-sV-Module manual.	2020-03-17
MiPNet23.15 O2k-pH ISE-Module		Measurement of proton flux with the O2k-pH ISE-Module.	2018-09-08
MiPNet22.11 O2k-FluoRespirometer manual	» Versions	O2k-FluoRespirometer manual.	2021-12-15
MiPNet19.18B POS-service	»Versions	Service of the polarographic oxygen sensor OroboPOS.	2021-06-23
MiPNet15.03 O2k-MultiSensor-ISE	»Versions	O2k-MultiSensor system with ion selective electrodes (ISE).	2021-08-02
MiPNet14.06 Instrumental O2 background	» Versions	O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux.	2020-04-16
MiPNet12.10 TIP2k-manual	»Versions	Titration-Injection microPump TIP2k manual.	2021-07-08
MiPNet07.08 User information	» Versions	O2k-Manual: user information. PLEASE STUDY THIS MANUAL.	2017-11-03
MiPNet06.03 POS-calibration-SOP	» Versions  » Versions	O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8. O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.	2021-08-19 2021-10-29

O2k-SOP

Chapter	View	Topic	Last update
MiPNet26.14 Oxia	» Versions	Oxia - HyperOxia to HypOxia.	2021-12-20
MiPNet06.03 POS-calibration-SOP	» Versions  » Versions	O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8. O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.	2021-08-19 2021-10-29
MiPNet19.03 O2k-cleaning and ISS	» Versions	O2k-Chamber cleaning SOP and Integrated Suction System (ISS).	2021-04-27
MiPNet20.04 O2k-checklist	» Versions	O2k-checklist: get started with an O2k-experiment.	2021-03-12
MiPNet14.06 Instrumental O2 background	» Versions	O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux.	2020-04-16
MiPNet19.14 SOP Hamilton microsyringes	» Versions	SOP for manual O2k-titrations with Hamilton microsyringes.	2020-02-24
MiPNet08.16 pH calibration	» Versions	Laboratory protocol: pH measurement and temperature dependence of pH.	2016-07-18
MiPNet06.06 Chemical O2 background	» Versions	Oxygraph assay of cytochrome c oxidase activity: chemical background correction.	2015-04-27

TIP2k Manual

Chapter	View	Section	Last update
MiPNet12.10 TIP2k-manual	»Versions	Titration-Injection microPump TIP2k manual.	2021-07-08

» O2k-Catalogue: TIP2k

» O2k-Publications: TIP2k

O2k-MultiSensor Manuals

Chapter	View	Section	Last update
MiPNet15.03 O2k-MultiSensor-ISE	»Versions	O2k-MultiSensor system with ion selective electrodes (ISE).	2021-08-02
MiPNet15.05 NO-manual	»Versions	O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.	2016-04-21
MiPNet17.05 O2k-Fluo LED2-Module	»Versions	O2k-Fluo LED2-Module.	2021-06-22
MiPNet23.15 O2k-pH ISE-Module		Measurement of proton flux with the O2k-pH ISE-Module.	2018-09-08
MiPNet24.12 NextGen-O2k: Q-Module	» Versions	NextGen-O2k: Q-Module manual	2021-10-29
MiPNet26.11 NextGen-O2k: PB-Module	» Versions	NextGen-O2k: PB-Module manual	2021-10-29
MiPNet26.12 NextGen-O2k: NADH-Module	» Versions	NextGen-O2k: NADH-Module manual	2021-10-29
MiPNet26.13 NextGen-O2k manual	» Versions	NextGen-O2k manual	2021-10-29

» O2k-pH ISE-Module: two pH electrodes and reference electrodes and accessories, two black PEEK stoppers, supported by the O2k-Core.

» O2k-Catalogue: O2k-MultiSensor

» O2k-Publications: O2k-Fluorometry

PBI-Shredder manual

Chapter	View	Section	Last update
MiPNet17.02 PBI-Shredder manual	» Versions	PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function.	2015-06-19

» O2k-Catalogue: O2k-Accessories and Auxiliary Tools

DatLab manuals

Chapter	View	Section	Last update
MiPNet06.03 POS-calibration-SOP	» Versions  » Versions	O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8. O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.	2021-08-19 2021-10-29
MiPNet07.08 User information	» Versions	O2k-Manual: user information. PLEASE STUDY THIS MANUAL.	2017-11-03
MiPNet12.10 TIP2k-manual	»Versions	Titration-Injection microPump TIP2k manual.	2021-07-08
MiPNet14.06 Instrumental O2 background	» Versions	O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux.	2020-04-16
MiPNet15.03 O2k-MultiSensor-ISE	»Versions	O2k-MultiSensor system with ion selective electrodes (ISE).	2021-08-02
MiPNet19.18B POS-service	»Versions	Service of the polarographic oxygen sensor OroboPOS.	2021-06-23
MiPNet22.11 O2k-FluoRespirometer manual	» Versions	O2k-FluoRespirometer manual.	2021-12-15
MiPNet23.15 O2k-pH ISE-Module		Measurement of proton flux with the O2k-pH ISE-Module.	2018-09-08
MiPNet24.14 O2k-sV-Module manual	»Versions	O2k-sV-Module manual.	2020-03-17

» O2k-Catalogue: DatLab

Data analysis with DatLab 7.4

Chapter	View	Section	Last update
MiPNet24.06 Oxygen flux analysis - DatLab 7.4	» Versions	Oxygen flux analysis with DatLab 7.4.	2020-04-16
MiPNet24.08 Safranin Analysis Template	» Versions	Excel template for safranin data analysis.	2020-05-13
MiPNet24.09 Data analysis of mt-membrane potential	» Versions	Data analysis of mt-membrane potential.	2021-09-16
MiPNet24.10 H2O2 flux analysis	»Versions	Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4	2021-10-22
MiPNet24.11 mtMP calculation	» Versions	Mitochondrial membrane potential calculation	2019-08-05
MiPNet26.06 DatLab 7: Guide	»Versions	DatLab-guide through the menus.	2021-06-02
MiPNet26.10 MgG data analysis	»Versions	Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4	2021-09-16

NextGen-O2k manuals and DatLab 8

Chapter	View	Section	Last update
MiPNet24.12 NextGen-O2k: Q-Module	» Versions	NextGen-O2k: Q-Module manual	2021-10-29
MiPNet24.16 DatLab8.0: CV-Module	»Versions	DatLab8.0: Cyclic voltammetry manual	2021-10-28
MiPNet26.11 NextGen-O2k: PB-Module	» Versions	NextGen-O2k: PB-Module manual	2021-10-29
MiPNet26.12 NextGen-O2k: NADH-Module	» Versions	NextGen-O2k: NADH-Module manual	2021-10-29
MiPNet26.13 NextGen-O2k manual	» Versions	NextGen-O2k manual	2021-10-29

DatLab 8 manuals

Chapter	View	Section	Last update
MiPNet06.03 POS-calibration-SOP	» Versions  » Versions	O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8. O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.	2021-08-19 2021-10-29
MiPNet24.12 NextGen-O2k: Q-Module	» Versions	NextGen-O2k: Q-Module manual	2021-10-29
MiPNet24.16 DatLab8.0: CV-Module	»Versions	DatLab8.0: Cyclic voltammetry manual	2021-10-28
MiPNet26.12 NextGen-O2k: NADH-Module	» Versions	NextGen-O2k: NADH-Module manual	2021-10-29
MiPNet26.13 NextGen-O2k manual	» Versions	NextGen-O2k manual	2021-10-29

O2k-Respirometer (Series A - Series G); DatLab 5 and 6

Chapter	View	Section	Last update
MiPNet17.05 O2k-Fluo LED2-Module	»Versions	O2k-Fluo LED2-Module.	2021-06-22
MiPNet19.01 O2k-Core Manual Series F DatLab 5	»Versions	O2k-Core Manual - up to O2k-Series F and DatLab 5.	2014-03
MiPNet19.18 O2k-Series G Core manual	»Versions	O2k-Core manual contents.	2016-08-08
MiPNet19.18A O2k-Series G: Start	» Versions	Oxygraph-2k: start high-resolution respirometry.	2016-08-08
MiPNet19.18C DatLab 6: Guide	»Versions	DatLab-guide through the menus.	2016-08-24
MiPNet19.18E O2 flux analysis - DatLab 6	»Versions	Oxygen flux analysis: DatLab real-time.	2016-08-08

» Oroboros O2k-Catalogue: O2k

O2k-Videosupport

» O2k-Videosupport

» O2k-Manual all chapters